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Questions related to Cellular Biology Techniques
I am doing a viability study using leukemia cells and planning on plating them into a 12 well plate. 1. I was wondering how plating efficiency can be calculated for floating cells such as leukemia...
12 December 2015 1,451 3 View
I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...
12 December 2015 7,111 1 View
Can anybody send me the sequences of promoter of Mus musculus Drd2 and STMN1 which has maximum Luciferase activity and any reference in this regard ?
12 December 2015 7,925 0 View
I have carried out the MTS test several times. The same dilution factor and sample. The results show that the absorbance of sample are higher than the control. When I plotted the graph percentage...
12 December 2015 1,745 7 View
We want to assess the frequency GARP and SATB1 in Tregs in HIV+ patients We are looking for a stimulant to use as a positive control.
12 December 2015 5,165 1 View
Hi friendly people! I've been doing live imaging of MDCK epithelial migration and I've noticed extreme variability in Hoechst staining. I use the NucBlue formulation where you just add 4...
12 December 2015 2,500 4 View
I was researching the hyperinsulinemic-euglysemic clamp in the conscious rat. All surgical procedures are performed under carotid artery and jugular vein and most of the papers suggest to use...
07 December 2015 3,859 2 View
Using the recommended media and temperature to grow the cells but they die following the first sub-culture. When revived from frozen they look very healthy but as soon as they are split most of...
11 November 2015 6,051 4 View
Hello All, I would like to ask if anyone know if measuring mitochondria number in cells could be feasible by using STORM microscopy to detect a specific fluorescent mitochondrial marker. If yes is...
11 November 2015 479 5 View
I am looking to conduct transport studies in CaCo-2 cells using three fluorescent dextrans namely, Fluorescein Salt (MW~3.7KD), FD4 (MW~ 3-5KD) and FD20S (MW~20KD). All three of them are from...
11 November 2015 4,260 4 View
I will load the cell free cholesterol, then I need extract the cholesterol from the cell. With WAKO kit to do lipid assay.
11 November 2015 4,807 6 View
Hi, I have isolated some mouse splenocyte and collected the pellets after isolation. I added QIAZOL into the cell pellet, but I forgot to pipette it up-and-down before I put it into the -80...
11 November 2015 489 8 View
I have constructed one mutant having kanamycin cassette. For the growth comparison between wild type and mutant of same species, is it essential to add kanamycin antibiotic in liquid medium? In...
11 November 2015 3,918 2 View
Cells where treated with Bafilo alone without any stresser or autophagy inducer
11 November 2015 4,795 6 View
Hi, I have a Sutter Puller P-97 and I am looking for protocols to pull glass capillaries (final tip diameter > 20 um) for injecting cells into tissues. Could somebody advice on which parameters...
11 November 2015 6,501 3 View
How can I carry out a 7 day cell-based proliferation assay? Is continuously refreshing the medium necessary, or should I just place it waiting for detection?
11 November 2015 4,417 19 View
What criteria do you use to surgery? Which embolization technique do you use?
09 November 2015 5,925 5 View
My friend asks: We have to block protein transport in vessel tissue culture, is it reasonable to incubate small pieces of tissue in collagenase/DNAse cocktail with Brefeldin A for about 1,5h...
10 October 2015 3,057 1 View
I am looking at PAR expression in our particular cell line. Unfortunately though, Trypsin, which is generally used for cell for cell detachment during passaging etc. is well known to activated...
10 October 2015 9,014 2 View
Hello Guys, Vortexing cell suspension for short time (~10sec) to homoginize solution before distribute cell into vessels. Can this damage the cell? Thank you Cheerio,
10 October 2015 4,437 5 View
Hello everyone, I am trying to get rid of hematopoietic lineage in bone marrow using the lineage depletion kit from Miltenyi. Without depletion, CD45-/Ter119- cells comprimise 8-10% of the whole...
10 October 2015 7,444 1 View
Using either MDCK, ARPE-19, or pig RPE cells.
10 October 2015 5,554 5 View
Looking for AAV-mediated expression of these opsins (see link)
10 October 2015 6,984 4 View
I tried to apply 3C experiment in NIH/3T3 cell line. NIH/3T3 is a Mouse Embryonic Fibroblast cell line. 1*10^7 cells were used for 3C experiment. However, by adding 0.3% sds in 1* NEB buffer 2.1,...
10 October 2015 6,830 3 View