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Questions related to Cellular Biology Techniques
IL-1 has not a typical secretory pathway, so If I want to detect IL-1 intracellular, can I use the classical protocols to assess it (I mean golgi stop)? regards
01 January 2016 315 2 View
U937 cells were treated with 200nM of PMA . After 3 days, the PMA-containing media was removed and replaced with fresh RPMI 1640 medium for a further 5 days. The final yield was very less.
01 January 2016 9,341 3 View
Hi, I'm wondering can I use H2O2 to induce the HT-29 cells into oxidative stress? Is it available to investigate the anti-oxidant factor by using HT-29 just like caco-2 cells? What's the...
01 January 2016 9,028 5 View
Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?
01 January 2016 6,731 2 View
Hi everybody!I'm working with protoplast from U. pinnatifida gametophyte. I was trying several methods only with commercial enzymes (cellulase R-10 and alginate lyase from Sigma) for isolation...
01 January 2016 459 2 View
Hi everyone, I am very new to MTT assay and I find myself stuck. I plated 5,000 cells/well in a 96-well plate. Following recovery and incubation, I added 50uL MTT reagent to each well and...
01 January 2016 5,230 6 View
Treatment of anti-cancer compounds on cancer cell increase the apoptosis. Can I detect pro, early and late apoptotic cell after treatment of inhibitor.
01 January 2016 10,039 6 View
I'm trying to detect total S-nitrosylation in SH-SY5Y cell culture using Sodium Nitroprusside (200, 400uM). I've treated cells with SNP for 15 min and isolated proteins using lysis buffer...
01 January 2016 10,156 0 View
What are the possible ways of basolateral-to-apical signal transitions for polarized epithelial cells in culture? I suppose, cytoskeleton is one of the sensing mechanisms, with basolateral...
01 January 2016 7,647 5 View
I tried to sort human neural stem cells (induced from hiPSC) and observed that almost all of the cells die after the sort, although they were alive before. I used 100um nozzle, and PBS-EB (EDTA...
01 January 2016 6,053 7 View
Has anybody had experience with MTT in non adherent cells?
01 January 2016 4,775 4 View
I am struggling to get fine cholesterol crystal for cell. I have got cholesterol crystal through the methods from many papers, but I cannot get very fine particles from relatively bigger...
01 January 2016 811 4 View
Does ethanol affect the stability of vesicles?For vesicular system removal of organic solvent is must, but in case of ethosomes we add 40 % ethanol.
01 January 2016 9,189 1 View
A lot of companies are marketing their Annexin/PI or Annexin/7-AAD kits as apoptosis/necrosis detection kits. However, can you really use these kits to separate between late stage apoptotic...
01 January 2016 1,060 4 View
I am using Ultracentrifugation technique for isolation of exosomes, does anyone have any idea how much protein can I get from 1 ml of mice plasma?
01 January 2016 318 5 View
It takes 40 minutes for a typical E. coli cell tocompletely replicate its chromosome. Simultaneousto the ongoing replication, 20 minutes of a freshround of replication is completed before the...
01 January 2016 8,268 0 View
I am doing a viability study using leukemia cells and planning on plating them into a 12 well plate. 1. I was wondering how plating efficiency can be calculated for floating cells such as leukemia...
12 December 2015 1,490 3 View
I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...
12 December 2015 7,140 1 View
Can anybody send me the sequences of promoter of Mus musculus Drd2 and STMN1 which has maximum Luciferase activity and any reference in this regard ?
12 December 2015 7,941 0 View
I have carried out the MTS test several times. The same dilution factor and sample. The results show that the absorbance of sample are higher than the control. When I plotted the graph percentage...
12 December 2015 1,823 7 View
We want to assess the frequency GARP and SATB1 in Tregs in HIV+ patients We are looking for a stimulant to use as a positive control.
12 December 2015 5,184 1 View
Hi friendly people! I've been doing live imaging of MDCK epithelial migration and I've noticed extreme variability in Hoechst staining. I use the NucBlue formulation where you just add 4...
12 December 2015 2,529 4 View
I was researching the hyperinsulinemic-euglysemic clamp in the conscious rat. All surgical procedures are performed under carotid artery and jugular vein and most of the papers suggest to use...
07 December 2015 3,874 2 View
Using the recommended media and temperature to grow the cells but they die following the first sub-culture. When revived from frozen they look very healthy but as soon as they are split most of...
11 November 2015 6,071 4 View