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Questions related to Cellular Biology Techniques
Hi guys, I want to measure microtubule bundlings, but I have no clue how to cope with this. Do you know some plugins or methods? Thank you very much for your input! Cheers, Feng
09 September 2015 657 3 View
Hi, I am nowing collecting the colon Lamina Propria cells. And when I prepared the digestion solution which need the DNase I. Here, I want to ask, I have two type of DNase I from different...
09 September 2015 1,223 2 View
I'm conducting an analysis of a phosphate transporter and I want to measure its transport capacity in human cells. I cannot use radioisotopes in this experiment.
09 September 2015 10,003 1 View
For example, I have a 8.603059e15 molecules of 70kDa dextran per mL of media into which I put HeLa or 3T3 cells, usually at either 1:1 or 1:3 ratio, trying to have them semi-buoyant... I was...
09 September 2015 2,546 1 View
I've been trying to fractionate glioblastoma stem cells (essentially transformed neural stem cells/progenitor cells) using either this Nature Methods protocol: Analysis of nuclear RNA interference...
09 September 2015 7,027 3 View
Hello~ I am trying to transfect GFP-N1-5-HT1a into N2a cells using lipofectamine 3000. Before, I tried GFP-C1-5-HT1a with the same experimental condition, and the transfection efficiency can be...
12 August 2015 6,061 6 View
Hi all. I have performed an MTT cell viability assay and the vehicle controls do not seem to be metabolizing the MTT reagent. I am basing this on the fact that I definitely see live adherent cells...
08 August 2015 6,557 7 View
I'm interested to know if there are any well known markers to study NETosis in neutrophils using IF?
08 August 2015 9,518 5 View
I am experiencing very low O.D values in MTT assay, when I measured using microplate reader at 570 nm, even though I took enough no of cells there is no much increase in O.D. is it quite common or...
08 August 2015 2,856 13 View
Ive noticed that when im using the biorad mini system for western blot there tends to be a small amount of overflow into adjoining wells when im loading the samples even though im loading often...
08 August 2015 6,005 5 View
Hi, I'm currently setting up T-DC co-cultures and am looking for advice. T cell line is IL2 dependent. T cell media contains IL2 (amongst other supplements), although DCs produce IL2. Any advice...
08 August 2015 6,796 11 View
What are the methods to study cell proliferation kinetics ?
08 August 2015 9,948 6 View
A eukaryotic protein is predicted to be secreted by the SignalP 4.0. And how can I determine wether the protein is indeed secreted or not by the experiment? Is there any simple assay can be done...
08 August 2015 8,731 6 View
Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When PI is bound to nucleic acids, the fluorescence...
08 August 2015 5,607 12 View
I am treating AR42J cells with 10-8 M cholecystokinin (CCK) for different time intervals up to 8 hours. Following this, I need to check for markers of cell death and for activation of the Unfolded...
14 July 2015 8,289 3 View
I know that in human cell-lines, some of the N-linked sugars that are added to proteins during maturation in the secretory pathway contain sialic-acid residues. This gives the N-linked sugars a...
07 July 2015 639 4 View
07 July 2015 7,387 4 View
Hello, I try to establish a 107 a assay unfortunately without success. I used human PBMCs and cocultured them with several cancer cells (with and without developed drugs). The flow results looked...
07 July 2015 1,219 1 View
I'm curious as to how to terminate protein expression in T7 Express Competent E. coli (high efficiency) after gene induction with 0.2% arabinose? Is there any way to halt this besides waiting for...
07 July 2015 4,396 3 View
The resistance reading from WPI EVOM2 is ohm x cm2 or just ohm? I read the manual very carefully and thought it's ohm x cm2 and we don't need to convert it. But the TER value I got for my RPE...
12 June 2015 5,258 2 View
How Type I IFN involvement of oxidative stress induce the DNA damage response of aging process?
06 June 2015 4,025 2 View
I am trying to detect stimulated Tregs and their IL-10 expression in cultured PBMCs (not isolated from the cells mixture) by flow cytometry. For other T cells phenotyping and evaluating of...
06 June 2015 9,927 10 View
I am trying to do clonogenic assay with HCt116, Ht29 and Panc1 cell lines. I did plating with different cell concentrations for HCT116 (200, 300, 500 and 1000/well in 6-well plates). I had good...
06 June 2015 3,747 13 View
I am studying the secretion of the gut hormone (GLP-1) using the NCI-H716 cell line. Since it is my first time to use this cell line in particuljsr and suspension cell line generally, I am...
06 June 2015 6,131 3 View