I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I am not able to understand the calculation behind that. Please help me to understand the calculation as well as the behaviour of the cells. Thank you.
I generally keep SF9 cells between 0.3-8E6 viable cells/ml for log phase growth. They tend to grow very fast and need routinely splitting every other day, down to 1E6 cells/ml. For splitting the cells; count them on whichever device you have present, this is the current density. You then need to decide what density you would like them at, for example on a Friday you may want to split them down to 0.3E6 cells/ml for weekend growth. You would therefore divide the required density (eg 0.3E6) / current density (eg 1.8E6) which gives you a 1/6 fraction. This means you need to add 1 part cells to 6 parts media to gain the new concentration.
- Badges
- Science topic
- Similar topics
- Methodology
- Laboratory
- Cellular Biology Techniques