I tried to sort human neural stem cells (induced from hiPSC) and observed that almost all of the cells die after the sort, although they were alive before. I used 100um nozzle, and PBS-EB (EDTA and 2% final BSA). And when I tried to isolate gDNA from the sorted cells, I could not obtain any, although there should have been 0.5M cells. 

Any ideas what would be stressing and killing them? Any suggestions to optimize it?

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