Is it correct to conduct two-way ANOVA when one combination of factors has only zero values?

Hello all! I have some problems while analyzing my data using two-way ANOVA approach. I have 2 factors (one with 2 levels and the other one with 4 levels) with 3 repetitions in each factor...

01 February 2019 9,131 6 View

More chl c than chl a content in brown algae?

Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...

20 March 2018 3,186 3 View

More chl c than chl a content in brown algae?

Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...

02 March 2018 5,256 4 View

What is the most suitable medium for testing axenic condition in macroalgae cultures?

06 July 2017 4,566 3 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

11 July 2016 2,155 6 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

06 July 2016 7,392 7 View

What is the difference between biological replicates, repetitions and replicates?

Hi everybody!I was checking some books and papers related to biostatistics and I found a no clear difference between biological replicates, replicates and repetitions (especially, between the two...

05 June 2016 2,344 13 View

What kind of mixture design can I use to know the best proportions of enzymes for protoplast isolation?

Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...

15 February 2016 4,408 2 View

A Laminariales gametophyte culture could be considered as a true cell suspension culture?

Hi everybody!I would like to ask your opinion (especially phycologist or anybody who works with plant cell suspension cultures) about this: as we know, Laminariales gametophytes (brown algae)...

01 February 2016 570 0 View

What kind of mixture design can I use to know the best proportions of enzymes for protoplast isolation?

Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...

01 February 2016 4,697 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View