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Questions related to Cellular Biology Techniques
The dynabead protocol suggests to use at least 100ul of antigen-containing lysate to resuspend the bead in immunoprecipitation. I usually 10ug of lysate dilluted in PBS to get 100ul and give me...
24 April 2015 5,392 5 View
Hi, I'm currently running DNA (300 bp) on 8% acrylamide gels for heteroduplex analysis. I loaded samples 2 times on the same gel (I loaded the first samples and let it run for 4 hrs and then I...
17 April 2015 3,620 11 View
I hope you can help me thanks
17 April 2015 6,277 3 View
What is the number of injected cells? and what is the tumor growth time ?
17 April 2015 6,213 2 View
I have subjected pieces of explanted liver samples for collagenase treatment (1/2 an hour at 37), and purified through Percoll centrifugation. However, the cells I obtained do not look polygonal,...
10 April 2015 2,423 3 View
I like Human Hek293 cells because they transfect so well - usually ~95% with Lipofectamine. However I need to work with a Rat cell line to test CRISPR gRNAs in and having a rat cell line that...
09 April 2015 6,077 11 View
hi,everybody.I'm just starting to do western blot,and so many problems came out. I just tried to transfer 250kda protein,but failed all the time.So I need some advices and to improve my...
09 April 2015 6,890 9 View
I read a paper. The authors performed in vivo coimmunoprecipitation to investigate the interaction between rsl1 (e3 ligase) and ABA receptor (putative substrate protein). ABA receptor band can be...
04 April 2015 7,243 2 View
Reading single cell papers made me wonder why researchers use 100 cells, 500 cells, or 1000 cells? What's criterion to call such numbers? How do you know you got the whole picture by using such a...
04 April 2015 5,062 7 View
04 April 2015 9,093 3 View
Hi, I am using DAPI for screening the nucleus in a tobacco cell line. However, when looking at the cells under microscope, DAPI is visualized mostly in the membrane and somehow in cytoplasm other...
04 April 2015 7,812 5 View
04 April 2015 2,334 5 View
I have tried using Mitlenyi's Gentlemacs with type III collagenase at 37C for about 45 minutes-60 minutes and I am fairly sure I am over-digesting the T cells. After processing to single cell...
04 April 2015 7,645 4 View
Thanks in advance for your replies.
04 April 2015 10,081 2 View
I have converted THP1 monocytes to macrophage using 5 ng/ml PMA for 48 hrs in complete RPMI1640.PMA Containing media was discarded and cells were washed with sterile DPBS for 3 times and given...
04 April 2015 5,339 2 View
I would like to determine 11beta HSD1 in PBMC
04 April 2015 3,821 1 View
04 April 2015 8,471 3 View
04 April 2015 10,132 12 View
Carbon catabolite repression caused by glucose is mediated through phosphotransferase system (PTS) and also known as "Glucose Repression".
04 April 2015 1,573 0 View
I'm trying to isolate oral epithelial cells, and currently using a modified protocol outlined by Izumi, Marcelo and Feinberg ( 18 hour incubation with Dispase followed by 2.5 hours with...
04 April 2015 7,008 0 View
I would like to know in what time of incubation for EBBS starvation is appropriate for human Peripheral mononuclear cells early time points or longer time points. Anybody have used MDC staning...
04 April 2015 3,719 2 View
Imbert and Cullander already published a method in 1999 to investigate tissue viability by MTT assay. The authors said tissue samples were incubated in 2 mL of 2-mg/mL MTT (dissolved in PBS) for 2...
04 April 2015 8,973 1 View
As HCl pre-treatment is carried out for BrdU staining in tissue sections and it disrupts the chromatin structure, will it cause any problem if we want to co-stain with any nuclear antigen marker?
04 April 2015 1,745 2 View
Which viability assays can I do to confirm chondrogenic differentiation from MSC pellets? Cells were pelleted in micro centrifuge tubes and differentiated.
31 March 2015 8,221 6 View