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Questions related to Cellular Biology Techniques
Hi all, I'm looking to isolate cells from mouse popliteal lymph nodes for rt-PCR down the line. I've tested a couple of different protocols and results have improved, but I believe my yields are...
03 March 2015 9,301 3 View
Hey, maybe some expert can help me. I did qPCR analysis of MEFs that showed severe differentiation defects and thus usually do not differentiate into adipocytes upon treatment with insulin...
03 March 2015 7,405 5 View
We have grown HT-29 cells (serum free) on non-coated transwells (8 um pore size, Greiner Bio) and incubated these at the basolateral side with 2.5, 5 and 10 ng/ml TGF-beta 1 for 12-24-48-72 hrs....
03 March 2015 931 12 View
I have tried several things so far, but I can't get it working. Either I have no LDL uptake at all or even my untreated macrophages are positive for Oil Red O staining. I was wondering if anyone...
03 March 2015 6,101 9 View
The hormone binding reaction was initiated by addition of fluorescent tagged natural ligand to each in 6 well plate (4 to 5 lac cells/ per well 6 well plate). Incubations were performed in...
03 March 2015 3,702 3 View
Hi everyone, I have recently purchased mitomycin C from SantaCruz to inactivate MEFs and since insufficient information was provided by the manufacturer on reconstitution and stability I had a...
28 February 2015 935 5 View
Hi All, A friend of mine from India has raised a concern since they don't have the transduction facility, what are the other ways to trace the transplanted Stem Cells in vivo in rodents if they...
28 February 2015 8,196 15 View
Observing a very frothy white layer on a bronchoalveolar fluid obtained with a very good yield (155/200ml) prompted me to measure both the albumin and protein content of this fluid. The beer-like...
26 February 2015 8,237 6 View
cell type is neutrophils. Thank you
24 February 2015 7,857 3 View
I am going to check the PGE2 secretion by using Prostaglandin E2 Express EIA KIT, cayman chemical. I am using it for the first time, so little hesitated. I have four samples, as below: (1) Control...
24 February 2015 985 2 View
I want to work on embryonic germ cells. Can anyone suggest from where I can procure these cells? Also, does anyone know the difference between embryonic germ cells and primordial germ cells
24 February 2015 5,235 1 View
Hello everybody, I am facing some major problems in transfecting a GFP::Protein in HeLa K cells. Briefly I am using a plasmid for transient expression in human cells, this plasmid has a pSV40...
23 February 2015 8,354 7 View
This solution would use in cell culture.
22 February 2015 7,335 6 View
I have performed few of the initial experiments for HUVEC cell migration in BD 24 well FluorBlock fibronectin coated plates. I procured the pre-screened primary HUVEC cells from Promocell. I am...
21 February 2015 1,176 7 View
We add resazurine to the following conditions: Just media (neg Control) Non-transfected cells (our positive control) Non-transfected cells + drug Transfected cells Transfected cells + drug To...
21 February 2015 2,253 6 View
We would like to do indirect BAT (ie incubating basophils with serum from allergic or sensitized patients) using a source of cells that can last awhile. After obtaining buffy coats which we Ficoll...
18 February 2015 8,148 1 View
I am currently performing hepatocyte isolation on mouse livers. Currently I have no issue with the isolation, but I am having problems with junk showing on my flow cytometry results. I am working...
18 February 2015 5,336 4 View
We don't have a 96well TEER measurement capability, but I wanted to make sure that the tight junctions are good prior to experimentation. Can lucifer yellow be used prior to the experiment to...
17 February 2015 7,612 5 View
Hi, everyone. I`m trying to activate NLRP3 inflammasome in various cell types with classical method (LPS + ATP). While I reviewed this process, I noticed that the concentration of ATP I`m using...
17 February 2015 6,612 4 View
I would perform a functional approach of the aquaporin 5 activity into the lung. Could you please help me ?
11 February 2015 4,668 8 View
The Alveolar–arterial gradient (A-aO2, or A–a gradient), is a measure of the difference between the alveolar concentration (A) and the arterial (a) partial pressure of oxygen. It seems an useful...
10 February 2015 1,466 11 View
Hello everybody. I would like to do measurements of ATP in pieces of liver from a KO mouse. I was wondering if anyone of you know a good buffer to solubilize liver pieces and then proceed with a...
05 February 2015 9,499 6 View
I use FITC dextran for the assessment of phagolysosomal fusion of fungal spores in bone marrow derived macrophages (BMDMs). I incubate BMDMs for 16 hours in 37oC, 5%CO2 with 0.5mg/ml FITC dextran....
02 February 2015 2,161 7 View
I am attempting to quantify a particular protein in red blood cells and I want to remove hemoglobin so that it will not interfere with the assay that I am using.
02 February 2015 2,782 9 View