Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?

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How can I visualize weekly fluorescent dye encapsulation with DOPC lipid?

Dear members, Can you please help me out, how to visualize dye encapsulated liposomes. As my dye is weekly fluorescent nature in lipid vesicle will it be useful to take fluorescence or con focal...

08 September 2016 2,510 2 View

How to prepare 1 mM solution of poly dI-dC from 10 U pack?

I would like to prepare 1 mM solution of polydI-dC (Poly(deoxyinosinic-deoxycytidylic) acid sodium salt). Please suggest me how to do this, here is the product...

08 September 2014 1,613 2 View

What is the Pka of (Hydroxypropyl)methyl cellulose) and how do I prepare the 1:1 stoichiometry of 1 mM soln of HPMC with NaOH soln?

I would like to prepare 1mM stock solution of HPMC with NaOH. The ratio of -CH-OH in HPMC and Na+ should be in 1:1 ratio. Please help me to prepare this solution.

08 September 2014 1,649 0 View

How do I convert ug/ml biopolymer to a uM solution?

I purchased poly dA-dT.poly dA-dT 10 unit packs from sigma and the concentration was found spectrophotometrically. I got a weight of this product in ug/ ml but I would like to prepare a uM...

08 September 2014 2,486 6 View

Can anybody suggest which type of HPLC column will be useful for separating Di positive carbocyanine dyes?

I would like to buy a column for separating my cyanine dyes. Is there any special columns will required to separate the positive charged dyes or C 18 columns will work for positive charged...

06 July 2014 9,504 2 View

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer avg Mw 250,000?

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer( avg Mw 250,000)

06 July 2014 1,531 5 View

How can I prepare 0.3 mM of Sodium carboxymethyl cellulose, average Mw ~250,000?

Will it follow the same molarity calculation as we do for small molecules, or is their any different calculations?

06 July 2014 7,322 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View