How can I visualize weekly fluorescent dye encapsulation with DOPC lipid?

08 September 2016 2,412 2 View

What is the Pka of (Hydroxypropyl)methyl cellulose) and how do I prepare the 1:1 stoichiometry of 1 mM soln of HPMC with NaOH soln?

I would like to prepare 1mM stock solution of HPMC with NaOH. The ratio of -CH-OH in HPMC and Na+ should be in 1:1 ratio. Please help me to prepare this solution.

08 September 2014 1,548 0 View

How to prepare 1 mM solution of poly dI-dC from 10 U pack?

I would like to prepare 1 mM solution of polydI-dC (Poly(deoxyinosinic-deoxycytidylic) acid sodium salt). Please suggest me how to do this, here is the product...

08 September 2014 1,472 2 View

How do I convert ug/ml biopolymer to a uM solution?

I purchased poly dA-dT.poly dA-dT 10 unit packs from sigma and the concentration was found spectrophotometrically. I got a weight of this product in ug/ ml but I would like to prepare a uM...

08 September 2014 2,330 6 View

Can anybody suggest which type of HPLC column will be useful for separating Di positive carbocyanine dyes?

06 July 2014 9,412 2 View

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer avg Mw 250,000?

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer( avg Mw 250,000)

06 July 2014 1,435 5 View

How can I prepare 0.3 mM of Sodium carboxymethyl cellulose, average Mw ~250,000?

Will it follow the same molarity calculation as we do for small molecules, or is their any different calculations?

06 July 2014 7,222 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View