Contact experts in Cellular Biology Techniques to get answers
1,924 views 442 posts
Questions related to Cellular Biology Techniques
I am treating C2C1 myotubes with free fatty acid and would like to measure mitochondrial specific super-oxide production. I will be using MitoTEMPO for specificity. I have couple of questions...
03 March 2015 9,024 17 View
I am interested to find out the composition of ILCs 3 in colon of GvHD patients. Since I only have paraffin embeded tissues, all I can do is staining cells and taking to microscope. It would be...
03 March 2015 297 6 View
We tried several times to isolate RNA from HK-2 cells with the qiagen RNeasy kit (with RLT buffer). We can do the isolation of RNA (with Qiagen kit) from other lines of human immortalized proximal...
03 March 2015 7,260 2 View
I need to check for the MLR (Mixed lymphocyte reaction) for the particular cells using MTT assay. I have to co-culture the responder cells (CD8 T cells) from one mouse strain with the stimulator...
03 March 2015 6,990 15 View
Hi I am trying to do double labeling of endothelial cells in FFPE tissues by using a Biotin conjugated reagent against a surface marker, and an indirect fluorescence approach (primary Ab and...
03 March 2015 8,603 15 View
Dear colleagues, I am trying to determine mitochondrial membrane potential with the help of TMRE but it's not working. I am following the protocol from abcam website which is incubating the cells...
03 March 2015 6,050 5 View
Hello I’m working on cell adhesion assay on fibronectin coating. I have prepared aliquote of fibronectin (50µg/ml of steril water) freezed at -20°C. However, when I thawed my aliquot, there was...
03 March 2015 6,393 5 View
I was using 10th day embryo, DMEM 450 ml, 10% FBS 50 ml, streptomycin 5 ml, tripsin for CEM culture. Three times I failed due to contamination. Finally, I got good cell growth in all the six well...
03 March 2015 2,201 4 View
Which viability assays can I do to confirm chondrogenic differentiation from MSC pellets? Cells were pelleted in micro centrifuge tubes and differentiated.
03 March 2015 6,327 7 View
Hi all, I'm looking to isolate cells from mouse popliteal lymph nodes for rt-PCR down the line. I've tested a couple of different protocols and results have improved, but I believe my yields are...
03 March 2015 9,312 3 View
Hey, maybe some expert can help me. I did qPCR analysis of MEFs that showed severe differentiation defects and thus usually do not differentiate into adipocytes upon treatment with insulin...
03 March 2015 7,417 5 View
We have grown HT-29 cells (serum free) on non-coated transwells (8 um pore size, Greiner Bio) and incubated these at the basolateral side with 2.5, 5 and 10 ng/ml TGF-beta 1 for 12-24-48-72 hrs....
03 March 2015 955 12 View
I have tried several things so far, but I can't get it working. Either I have no LDL uptake at all or even my untreated macrophages are positive for Oil Red O staining. I was wondering if anyone...
03 March 2015 6,115 9 View
The hormone binding reaction was initiated by addition of fluorescent tagged natural ligand to each in 6 well plate (4 to 5 lac cells/ per well 6 well plate). Incubations were performed in...
03 March 2015 3,719 3 View
Hi everyone, I have recently purchased mitomycin C from SantaCruz to inactivate MEFs and since insufficient information was provided by the manufacturer on reconstitution and stability I had a...
28 February 2015 972 5 View
Hi All, A friend of mine from India has raised a concern since they don't have the transduction facility, what are the other ways to trace the transplanted Stem Cells in vivo in rodents if they...
28 February 2015 8,209 15 View
Observing a very frothy white layer on a bronchoalveolar fluid obtained with a very good yield (155/200ml) prompted me to measure both the albumin and protein content of this fluid. The beer-like...
26 February 2015 8,258 6 View
cell type is neutrophils. Thank you
24 February 2015 7,872 3 View
I am going to check the PGE2 secretion by using Prostaglandin E2 Express EIA KIT, cayman chemical. I am using it for the first time, so little hesitated. I have four samples, as below: (1) Control...
24 February 2015 1,003 2 View
I want to work on embryonic germ cells. Can anyone suggest from where I can procure these cells? Also, does anyone know the difference between embryonic germ cells and primordial germ cells
24 February 2015 5,253 1 View
Hello everybody, I am facing some major problems in transfecting a GFP::Protein in HeLa K cells. Briefly I am using a plasmid for transient expression in human cells, this plasmid has a pSV40...
23 February 2015 8,373 7 View
This solution would use in cell culture.
22 February 2015 7,364 6 View
I have performed few of the initial experiments for HUVEC cell migration in BD 24 well FluorBlock fibronectin coated plates. I procured the pre-screened primary HUVEC cells from Promocell. I am...
21 February 2015 1,189 7 View
We add resazurine to the following conditions: Just media (neg Control) Non-transfected cells (our positive control) Non-transfected cells + drug Transfected cells Transfected cells + drug To...
21 February 2015 2,277 6 View