I was researching the hyperinsulinemic-euglysemic clamp in the conscious rat. All surgical procedures are performed under carotid artery and jugular vein and most of the papers suggest to use these. It is important to isolate the vagus nerve from the artery without damaging either the artery or the nerve. Because of reason, is it possible to perform correctly under femoral artery and vein? Advantages and disadvantages? Can glucose levels can easily be compensated?