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Questions related to Cellular Biology Techniques
In our proposed hypothesis (2005), the leading role in the development attacks of breathlessness in patients with bronchial asthma given to hyperactivity of the bronchi. The specific immune...
07 May 2016 907 4 View
I normally starve cells for 24 hours in serum free media and then add my drug in serum media, scratch and add this treatment media and follow the healing. In case of HaCat I starved the cell in 1%...
23 April 2016 952 3 View
For the clear plaques, I can understand it's a complete lysis, no bacteria are growing in that aera any more. But how to explain the turbid plaques? Is it the phage lysed some of the bacteria, but...
19 April 2016 6,379 17 View
how to monitor tumor propagation in lung after inoculation of cell lines in the lung. Is there any simple and reliable method by which it can be check the gradual increase in the size of tumor in...
29 March 2016 2,250 2 View
Does anyone try to induce inflammasome activation with LPS ATP and detect cell death with LDH and obtain discordant results?
16 March 2016 2,939 7 View
I have isolated exosomes from conditioned cell culture media . I have to go for its downstream applications and need to do the MTT assay. For that how to quantify the exosomes. Can we go with...
02 March 2016 4,720 12 View
I am interested in doing a western blot to detect expression of focal adhesion proteins in cells that are encapsulated in alginate hydrogels. The problem seems to be the infiltration of e.g. SDS...
07 February 2016 1,421 7 View
Hi everyone, I am very new to MTT assay and I find myself stuck. I plated 5,000 cells/well in a 96-well plate. Following recovery and incubation, I added 50uL MTT reagent to each well and...
25 January 2016 4,453 5 View
IL-1 has not a typical secretory pathway, so If I want to detect IL-1 intracellular, can I use the classical protocols to assess it (I mean golgi stop)? regards
01 January 2016 267 2 View
U937 cells were treated with 200nM of PMA . After 3 days, the PMA-containing media was removed and replaced with fresh RPMI 1640 medium for a further 5 days. The final yield was very less.
01 January 2016 9,288 3 View
Hi, I'm wondering can I use H2O2 to induce the HT-29 cells into oxidative stress? Is it available to investigate the anti-oxidant factor by using HT-29 just like caco-2 cells? What's the...
01 January 2016 8,968 5 View
Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?
01 January 2016 6,672 2 View
Hi everybody!I'm working with protoplast from U. pinnatifida gametophyte. I was trying several methods only with commercial enzymes (cellulase R-10 and alginate lyase from Sigma) for isolation...
01 January 2016 385 2 View
01 January 2016 5,169 6 View
Treatment of anti-cancer compounds on cancer cell increase the apoptosis. Can I detect pro, early and late apoptotic cell after treatment of inhibitor.
01 January 2016 9,979 6 View
I'm trying to detect total S-nitrosylation in SH-SY5Y cell culture using Sodium Nitroprusside (200, 400uM). I've treated cells with SNP for 15 min and isolated proteins using lysis buffer...
01 January 2016 10,083 0 View
What are the possible ways of basolateral-to-apical signal transitions for polarized epithelial cells in culture? I suppose, cytoskeleton is one of the sensing mechanisms, with basolateral...
01 January 2016 7,583 5 View
I tried to sort human neural stem cells (induced from hiPSC) and observed that almost all of the cells die after the sort, although they were alive before. I used 100um nozzle, and PBS-EB (EDTA...
01 January 2016 5,978 7 View
Has anybody had experience with MTT in non adherent cells?
01 January 2016 4,669 4 View
I am struggling to get fine cholesterol crystal for cell. I have got cholesterol crystal through the methods from many papers, but I cannot get very fine particles from relatively bigger...
01 January 2016 760 4 View
Does ethanol affect the stability of vesicles?For vesicular system removal of organic solvent is must, but in case of ethosomes we add 40 % ethanol.
01 January 2016 9,120 1 View
A lot of companies are marketing their Annexin/PI or Annexin/7-AAD kits as apoptosis/necrosis detection kits. However, can you really use these kits to separate between late stage apoptotic...
01 January 2016 979 4 View
I am using Ultracentrifugation technique for isolation of exosomes, does anyone have any idea how much protein can I get from 1 ml of mice plasma?
01 January 2016 258 5 View
It takes 40 minutes for a typical E. coli cell tocompletely replicate its chromosome. Simultaneousto the ongoing replication, 20 minutes of a freshround of replication is completed before the...
01 January 2016 8,217 0 View