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Questions related to Cellular Biology Techniques
For the qunatitaive study of atherosclerosis in mice, is gelatin embedding of heart important or can we do snap freeze with isopentane? My understanding is that, to study atherosclerosis, we need...
27 January 2017 5,841 1 View
Hey, I do have a question with murine GM-CSF and M-CSF for making rat BMDCs and BMDMs? I want to use WEHI 3B and L929 conditioned cell culture media in order to generate rat BMDCs and BMDMs. Is...
24 January 2017 2,760 3 View
Cell viability assayHepG2 and Huh-7 cells and primary human hepatocyteswere seeded in 96-well plates at 1 × 104 cells per welland cultured for 24 h. The cells were subsequentlystarved in culture...
24 January 2017 522 10 View
Hello, I have soft-tissue samples from different organs stored at -80 (previously snap-frozen in liquid nitrogen). I would need to isolate cell nuclei and fix on slides and I need the nucleus...
21 January 2017 8,140 6 View
I am making hypoxia situation in human pigment epithelial (RPE)using CoCl2. I read the some paper that the cobalt could make hypoxia situation and reduce the cell viability. however i used from...
18 January 2017 5,226 4 View
Is 4 hours too long? Would the MFI signal decrease by more than 50% of the expected value?
18 January 2017 3,253 4 View
After centrifuging in a v-bottom 96-well, at 2000rpm (800G), 3min, 4deg, (volume about 180ul) I added 180ul buffer without breaking up the pellet, and then centrifuged the cells again. (i.e i...
18 January 2017 1,064 1 View
Which artery to ligate for creating a Rat model of Ischemia reperfusion injury for heart?
03 October 2016 5,868 3 View
Im working on the targeting of NPs to specific tumor cell and to relase the payloads there.
25 August 2016 9,022 3 View
Dear All, I observed a 58 yo female patient in deep pain and was diagnosed as having a left-sided atypical chest pain. And she was sent for a chest X-Ray. It was found that she has a 1.0 cm x 0.8...
10 June 2016 9,782 6 View
Hi, I need to purchase non small cell lung cancer (NSCLC) fresh tissues and matched blood. I am looking for a company in Europe (preferably in Germany). Moreover I would like to know which is the...
07 June 2016 9,067 8 View
http://www.sigmaaldrich.com/catalog/product/sigma/l7647?lang=en®ion=US
01 June 2016 9,882 3 View
I have been intubating my rats for stereotaxtic surgery, however, recently the company (Excel International) that made the caterers I was using have altered their product. They are no longer rigid...
31 May 2016 4,182 8 View
Can the use of different types of mechanical ventilatory modes cause peripheral vasoconstriction? During the cares of a patient on BIPAP ASB it was noted that the patients fingers were both cold...
15 May 2016 8,301 3 View
In our proposed hypothesis (2005), the leading role in the development attacks of breathlessness in patients with bronchial asthma given to hyperactivity of the bronchi. The specific immune...
07 May 2016 975 4 View
I normally starve cells for 24 hours in serum free media and then add my drug in serum media, scratch and add this treatment media and follow the healing. In case of HaCat I starved the cell in 1%...
23 April 2016 1,009 3 View
I am working on Liver ultrasound images and want to extract fractal dimension. Need to know how to binarize the image so that best fractal value comes out. Binarization of image is critical part....
22 April 2016 8,201 3 View
For the clear plaques, I can understand it's a complete lysis, no bacteria are growing in that aera any more. But how to explain the turbid plaques? Is it the phage lysed some of the bacteria, but...
19 April 2016 6,435 17 View
how to monitor tumor propagation in lung after inoculation of cell lines in the lung. Is there any simple and reliable method by which it can be check the gradual increase in the size of tumor in...
29 March 2016 2,300 2 View
Does anyone try to induce inflammasome activation with LPS ATP and detect cell death with LDH and obtain discordant results?
16 March 2016 2,992 7 View
Can anyone share what G catheter they are using for what exact weight/age animals for femoral artery catheterization? Thank you!
06 March 2016 3,254 2 View
I have isolated exosomes from conditioned cell culture media . I have to go for its downstream applications and need to do the MTT assay. For that how to quantify the exosomes. Can we go with...
02 March 2016 4,788 12 View
I am interested in doing a western blot to detect expression of focal adhesion proteins in cells that are encapsulated in alginate hydrogels. The problem seems to be the infiltration of e.g. SDS...
07 February 2016 1,485 7 View
Hi everyone, I am very new to MTT assay and I find myself stuck. I plated 5,000 cells/well in a 96-well plate. Following recovery and incubation, I added 50uL MTT reagent to each well and...
25 January 2016 4,495 5 View