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Questions related to Cellular Biology Techniques
I have carried out the MTS test several times. The same dilution factor and sample. The results show that the absorbance of sample are higher than the control. When I plotted the graph percentage...
12 December 2015 1,560 7 View
We want to assess the frequency GARP and SATB1 in Tregs in HIV+ patients We are looking for a stimulant to use as a positive control.
12 December 2015 5,095 1 View
Hi friendly people! I've been doing live imaging of MDCK epithelial migration and I've noticed extreme variability in Hoechst staining. I use the NucBlue formulation where you just add 4...
12 December 2015 2,401 4 View
Using the recommended media and temperature to grow the cells but they die following the first sub-culture. When revived from frozen they look very healthy but as soon as they are split most of...
11 November 2015 5,996 4 View
Hello All, I would like to ask if anyone know if measuring mitochondria number in cells could be feasible by using STORM microscopy to detect a specific fluorescent mitochondrial marker. If yes is...
11 November 2015 369 5 View
I am looking to conduct transport studies in CaCo-2 cells using three fluorescent dextrans namely, Fluorescein Salt (MW~3.7KD), FD4 (MW~ 3-5KD) and FD20S (MW~20KD). All three of them are from...
11 November 2015 4,191 4 View
I will load the cell free cholesterol, then I need extract the cholesterol from the cell. With WAKO kit to do lipid assay.
11 November 2015 4,748 6 View
Hi, I have isolated some mouse splenocyte and collected the pellets after isolation. I added QIAZOL into the cell pellet, but I forgot to pipette it up-and-down before I put it into the -80...
11 November 2015 388 8 View
I have constructed one mutant having kanamycin cassette. For the growth comparison between wild type and mutant of same species, is it essential to add kanamycin antibiotic in liquid medium? In...
11 November 2015 3,852 2 View
Cells where treated with Bafilo alone without any stresser or autophagy inducer
11 November 2015 4,741 6 View
Hi, I have a Sutter Puller P-97 and I am looking for protocols to pull glass capillaries (final tip diameter > 20 um) for injecting cells into tissues. Could somebody advice on which parameters...
11 November 2015 6,426 3 View
How can I carry out a 7 day cell-based proliferation assay? Is continuously refreshing the medium necessary, or should I just place it waiting for detection?
11 November 2015 4,364 19 View
My friend asks: We have to block protein transport in vessel tissue culture, is it reasonable to incubate small pieces of tissue in collagenase/DNAse cocktail with Brefeldin A for about 1,5h...
10 October 2015 2,983 1 View
I am looking at PAR expression in our particular cell line. Unfortunately though, Trypsin, which is generally used for cell for cell detachment during passaging etc. is well known to activated...
10 October 2015 8,960 2 View
Hello Guys, Vortexing cell suspension for short time (~10sec) to homoginize solution before distribute cell into vessels. Can this damage the cell? Thank you Cheerio,
10 October 2015 4,349 5 View
Hello everyone, I am trying to get rid of hematopoietic lineage in bone marrow using the lineage depletion kit from Miltenyi. Without depletion, CD45-/Ter119- cells comprimise 8-10% of the whole...
10 October 2015 7,398 1 View
Using either MDCK, ARPE-19, or pig RPE cells.
10 October 2015 5,498 5 View
Looking for AAV-mediated expression of these opsins (see link)
10 October 2015 6,921 4 View
I tried to apply 3C experiment in NIH/3T3 cell line. NIH/3T3 is a Mouse Embryonic Fibroblast cell line. 1*10^7 cells were used for 3C experiment. However, by adding 0.3% sds in 1* NEB buffer 2.1,...
10 October 2015 6,772 3 View
1) Acetaldehyde and acrolein both are very evaporable and there are some loss during the solution preparation and incubation with cell in the 96-well plates or other plates. So A549 cell...
10 October 2015 3,706 3 View
Their molecular structures are quite similar, but I have really no idea about such replacement...
10 September 2015 2,709 3 View
HI everyone, Could I have your opinion on these photos please ? I revived my HEK293T cells yesterday in FreeStyle medium and I noticed today those black floating vesicles. I had contamination...
09 September 2015 708 4 View
I am looking for a method to totally suppress RNA transcriptase in cell suspensions and see if any new strands of RNA are still elaborated as measured by uptake of tritiated nucleotides.
09 September 2015 6,698 6 View
Culturing primary cells from the nose we are constantly dealing with a loss of cultures due to persisting primary contamination after the first 3 days period (protocol according to ller L,...
09 September 2015 960 1 View