Contact experts in Cellular Biology Techniques to get answers
1,533 views 395 posts
Questions related to Cellular Biology Techniques
I am presently using Freeze thaw method, giving only 5-10% . Suggest a better method.
07 May 2017 2,839 3 View
I am trying to perform colonogenic assay on MDA-MB-231 cells. After 14 days of treatment, I wash cells with PBS and add 0.1% crystal violet in methanol to the cells. I stain them for 30 minutes in...
07 May 2017 3,043 3 View
Hi Guys, I cannot find anything in literature. I have olny found a paper in yeast.. I see that K63 ubiquitination decreases upon mg132 treatment. I would have expected an increase...any cues? Tnx...
04 May 2017 726 3 View
I am working on a project where I have an image with a group of cells and I need to count them.
30 April 2017 4,058 5 View
I am currently working on DLBCL cell lines, and trying to isolate RNA from exosomes of cell lines. I isolated the exosomes from cell lines using the standard protocol of ultracentrifugation and...
17 April 2017 7,116 3 View
Good day all, Whats the mechanism by which the Nuclear Matrix Protein 4 can control the UPR? Thanks
17 April 2017 2,457 2 View
The cells were previously thawed 4/7 and then seeded at 0.4e6/mL until 4/10 then the count was 1.5e6/mL. Diluted them to 1e6/mL and grew overnight. Today the count is still at 1e6/mL. Why didn't...
11 April 2017 5,769 1 View
Could someone kindly explain to me why some protocol do not wash the cells after staining with FITC-Annexin V and PI, to get rid of unbound annexin V/PI ?
30 March 2017 3,555 2 View
We use the BrdU ELISA Kit from Roche a lot & never had any problems with it. Somehow, now we observe crystal formation (dark violett to black crystals) in the last steps of the assay... we did...
27 March 2017 3,758 2 View
Hi, I am performing UV irradiation studies on human fibroblasts. I want to perform IF after UV exposure and a short recovery time, which would follow pre - extraction at 4 degrees and PFA 4%...
21 March 2017 3,645 1 View
Annexin/PI for attached cell lines
16 March 2017 5,425 2 View
Hello all, I want to use Ki67 along with IFN, TNF in relation to T cell inhibitors like PD-1. If I use PBMC's and stim with PMA/iono for 6 hours with golgi inhibitors I am unable to see Ki67...
28 February 2017 6,142 4 View
Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...
22 February 2017 672 2 View
Hi, everyone! Recently I'm collecting lentivirus. After package in 293T cells, I take the medium, centrifuge at 1000rpm for 5 min to pellet the cells, then take the supernatant and filter by...
19 February 2017 7,298 5 View
I had to work with HeLa cells, and I have a question related to cell culture work. What would happen if I wash HeLa cells with water? I am asking this as when i wash the cells with water, all...
03 February 2017 9,914 4 View
I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP) Any suggestion on how to reduce? Thank you!
31 January 2017 6,074 1 View
Pictures taken at 200x
27 January 2017 3,055 7 View
Hey, I do have a question with murine GM-CSF and M-CSF for making rat BMDCs and BMDMs? I want to use WEHI 3B and L929 conditioned cell culture media in order to generate rat BMDCs and BMDMs. Is...
24 January 2017 2,646 3 View
Cell viability assayHepG2 and Huh-7 cells and primary human hepatocyteswere seeded in 96-well plates at 1 × 104 cells per welland cultured for 24 h. The cells were subsequentlystarved in culture...
24 January 2017 365 10 View
Hello, I have soft-tissue samples from different organs stored at -80 (previously snap-frozen in liquid nitrogen). I would need to isolate cell nuclei and fix on slides and I need the nucleus...
21 January 2017 8,089 6 View
I am making hypoxia situation in human pigment epithelial (RPE)using CoCl2. I read the some paper that the cobalt could make hypoxia situation and reduce the cell viability. however i used from...
18 January 2017 5,162 4 View
Is 4 hours too long? Would the MFI signal decrease by more than 50% of the expected value?
18 January 2017 3,151 4 View
After centrifuging in a v-bottom 96-well, at 2000rpm (800G), 3min, 4deg, (volume about 180ul) I added 180ul buffer without breaking up the pellet, and then centrifuged the cells again. (i.e i...
18 January 2017 975 1 View
I normally starve cells for 24 hours in serum free media and then add my drug in serum media, scratch and add this treatment media and follow the healing. In case of HaCat I starved the cell in 1%...
23 April 2016 914 3 View