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Questions related to Cellular Biology Techniques
I'm working on a research for the university so i'm wondering does the artery return back the same if we didn't use a stent after inflate a balloon ? and why it's better to udse a stent that made...
12 December 2017 611 1 View
Good day to all, I have been having trouble plating my HeLa cells into Ibidi 8-well chambers for confocal imaging. My HeLa cell stocks have been growing fine in T-75 flasks, however, they do not...
04 September 2017 9,347 3 View
Hi, I'm trying to determine a sub-lethal dose of hydrogen peroxide to use for my cells (skin fibroblasts and keratinocytes) for subsequent respirometry experiments. I'm planning to expose the...
01 August 2017 7,408 3 View
I have checked the peroxidase activity of E.coli under the stress of silver nitrate (20 μg/ ml). Surprisingly, I am getting negative results means control sample (E.coli without any silver nitrate...
28 July 2017 2,000 3 View
I'm currently running a phagocytic assay in which FITC-labeled E.coli bioparticles are being engulfed by lymphocytes. When running my control samples, however, the particles appear so bright that...
26 July 2017 2,136 3 View
Hi, I've been performing a kill curve assay for puromycin with 293T cells. 5 days post puro addition, it appears that the vast majority of cells (90+%) had detached, forming very large 'mats' of...
24 July 2017 827 2 View
I would like to know which volumes You use to split a 100 % confluent 150 mm COS-1 cells dish in order to obtain an 80% confluent 150 mm dish 24 h after splting. Which volumes of tripsine do You...
20 July 2017 9,747 3 View
I work with Giardia intestinalis and I'm trying to perform an IFA on those cells, but no matter how many cells I pipette on the coverslip they tend to fall off, and I end up with very few cells,...
06 July 2017 4,740 2 View
What are the substitutes to Sodium azide and EMS as cytotoxic agents?
02 July 2017 7,135 1 View
I am using CCCP as a mitochondrial stress inducing agent for 16hrs at 0.1uM conc. in HeLa cells. I am interested to know about the half life of CCCP in HeLa. Is it more than 16h ?
25 June 2017 4,001 2 View
Is there any relation between left atrial appendage & left ventricle during passive contraction of the left atrial appendage?
24 June 2017 8,802 0 View
After stimulation and incubation of our PBMCs for 24 hours we harvest 120ul of cell suspension (600.000 cells), centrifuge it, discard supernatant, and resuspend the cell pellet with 100ul mix of...
22 June 2017 1,689 3 View
Hello everyone, often i observe large cells (approximately 4 or 5 times the size of the other cancer cells) in my cell culture. Does anyone has a clue what's happening with these cells? Also i...
22 June 2017 9,995 2 View
I need help the the steps and the math required to dilute a concentration of cells (4.0x10^5 cells/mL) across 6 wells. I would like to have increments of .5x10^5 (50,000) so the first well will...
15 June 2017 912 1 View
I am wondering if I should fix and permeabilize my cells with a 50:50 mixture of ice cold methanol and acetone when I perform transwell migration assays. I have seen most methods mention using...
14 June 2017 8,426 2 View
Dear All I intend to use mitosis index, using DAPI staining, to measure synchrony level of plant cell suspension after treatments. Size of aggregates in the cell suspension are less than 500...
12 June 2017 6,511 11 View
I need to study the SOD level in granulosa cells of the cumulus-oocyte complex. Can any experts tell me how to lyse the cells and count for 5 million cells/vial?
11 June 2017 3,908 3 View
Respected members My junior want to start work on Balsam in respect of gamma irradiation. So please help us
19 May 2017 1,849 3 View
I treat arabidopsis seedlings in liquid culture with flagellin and want to track pH changes over 40 min by taking aliquots of 100 ul and measuring pH. Any other idea to do so is appriciated. Thanks
18 May 2017 9,521 1 View
I am presently using Freeze thaw method, giving only 5-10% . Suggest a better method.
07 May 2017 2,899 3 View
I am trying to perform colonogenic assay on MDA-MB-231 cells. After 14 days of treatment, I wash cells with PBS and add 0.1% crystal violet in methanol to the cells. I stain them for 30 minutes in...
07 May 2017 3,115 3 View
Hi Guys, I cannot find anything in literature. I have olny found a paper in yeast.. I see that K63 ubiquitination decreases upon mg132 treatment. I would have expected an increase...any cues? Tnx...
04 May 2017 814 3 View
I am working on a project where I have an image with a group of cells and I need to count them.
30 April 2017 4,125 5 View
I am currently working on DLBCL cell lines, and trying to isolate RNA from exosomes of cell lines. I isolated the exosomes from cell lines using the standard protocol of ultracentrifugation and...
17 April 2017 7,192 3 View