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Questions related to Cellular Biology Techniques
I have been working with NLRP3 inflammasome signalling and have been using RAW 264.7 murine macrophage cells stimulated with LPS to do so. I have recently come across several sources asserting...
14 May 2019 7,413 2 View
An obvious answer would be tar, but I can't find any analytical evidence for this. I have even found some weak evidence* against this. It seems that it's not even one particular...
12 January 2019 6,355 4 View
To my knowledge, a discussion of body orientation is curiously absent from the literature (cf. Hooker and Fahlman 2016) on lung (alveolar) collapse at depth (two exceptions, see below). The...
01 January 2019 4,121 0 View
We increase vital capacity by increasing chest wall flexibility. https://bit.ly/2Dus0XM
17 November 2018 8,978 0 View
What is the simplest, quickest, and/or cheapest way of finding out whether a molecule is involved in a novel quorum sensing / cell-cell signaling / signal transduction pathway? Most literature...
17 July 2018 4,760 3 View
Hi I am studying the role of my protein in lung cancer and using A549 (epithelial lung adenocarcinoma) and H1299 (Non small lung cancer ). Could someone please suggest what would be the most...
17 April 2018 4,527 3 View
what is the best management in Acute exacerbation of COPD which is resistant to medical management : A- CPAP ( Non invasive positve pressure ventilation ) B- Intubation and mechanical Ventilation .
19 February 2018 5,742 11 View
Does anyone know where I might find an chronic kidney disease ultrasound image database?
23 January 2018 1,121 7 View
I just really don't know why my results isn't equal when I use CellTiter-Glo and CellTiter-Glo 2. Can someone explain it to me?
16 January 2018 3,987 1 View
I'm working on a research for the university so i'm wondering does the artery return back the same if we didn't use a stent after inflate a balloon ? and why it's better to udse a stent that made...
12 December 2017 630 1 View
Good day to all, I have been having trouble plating my HeLa cells into Ibidi 8-well chambers for confocal imaging. My HeLa cell stocks have been growing fine in T-75 flasks, however, they do not...
04 September 2017 9,375 3 View
Hi, I'm trying to determine a sub-lethal dose of hydrogen peroxide to use for my cells (skin fibroblasts and keratinocytes) for subsequent respirometry experiments. I'm planning to expose the...
01 August 2017 7,436 3 View
I have checked the peroxidase activity of E.coli under the stress of silver nitrate (20 μg/ ml). Surprisingly, I am getting negative results means control sample (E.coli without any silver nitrate...
28 July 2017 2,027 3 View
I'm currently running a phagocytic assay in which FITC-labeled E.coli bioparticles are being engulfed by lymphocytes. When running my control samples, however, the particles appear so bright that...
26 July 2017 2,161 3 View
Hi, I've been performing a kill curve assay for puromycin with 293T cells. 5 days post puro addition, it appears that the vast majority of cells (90+%) had detached, forming very large 'mats' of...
24 July 2017 849 2 View
I would like to know which volumes You use to split a 100 % confluent 150 mm COS-1 cells dish in order to obtain an 80% confluent 150 mm dish 24 h after splting. Which volumes of tripsine do You...
20 July 2017 9,766 3 View
After modifying the Corometrics Nautilus Ultrasound cable (TFCU-CM03-0101) to break out pens 3, 4, and 5 as shown in table 5-9 below. This cable was modified such that the ultrasound cable would...
09 July 2017 8,648 0 View
I work with Giardia intestinalis and I'm trying to perform an IFA on those cells, but no matter how many cells I pipette on the coverslip they tend to fall off, and I end up with very few cells,...
06 July 2017 4,772 2 View
What are the substitutes to Sodium azide and EMS as cytotoxic agents?
02 July 2017 7,159 1 View
I am using CCCP as a mitochondrial stress inducing agent for 16hrs at 0.1uM conc. in HeLa cells. I am interested to know about the half life of CCCP in HeLa. Is it more than 16h ?
25 June 2017 4,020 2 View
Is there any relation between left atrial appendage & left ventricle during passive contraction of the left atrial appendage?
24 June 2017 8,830 0 View
After stimulation and incubation of our PBMCs for 24 hours we harvest 120ul of cell suspension (600.000 cells), centrifuge it, discard supernatant, and resuspend the cell pellet with 100ul mix of...
22 June 2017 1,719 3 View
Hello everyone, often i observe large cells (approximately 4 or 5 times the size of the other cancer cells) in my cell culture. Does anyone has a clue what's happening with these cells? Also i...
22 June 2017 10,023 2 View
I need help the the steps and the math required to dilute a concentration of cells (4.0x10^5 cells/mL) across 6 wells. I would like to have increments of .5x10^5 (50,000) so the first well will...
15 June 2017 950 1 View