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Questions related to Cellular Biology Techniques
Culturing primary cells from the nose we are constantly dealing with a loss of cultures due to persisting primary contamination after the first 3 days period (protocol according to ller L,...
09 September 2015 1,076 1 View
Hi guys, I want to measure microtubule bundlings, but I have no clue how to cope with this. Do you know some plugins or methods? Thank you very much for your input! Cheers, Feng
09 September 2015 795 3 View
Hi, I am nowing collecting the colon Lamina Propria cells. And when I prepared the digestion solution which need the DNase I. Here, I want to ask, I have two type of DNase I from different...
09 September 2015 1,358 2 View
I'm conducting an analysis of a phosphate transporter and I want to measure its transport capacity in human cells. I cannot use radioisotopes in this experiment.
09 September 2015 10,151 1 View
For example, I have a 8.603059e15 molecules of 70kDa dextran per mL of media into which I put HeLa or 3T3 cells, usually at either 1:1 or 1:3 ratio, trying to have them semi-buoyant... I was...
09 September 2015 2,644 1 View
I've been trying to fractionate glioblastoma stem cells (essentially transformed neural stem cells/progenitor cells) using either this Nature Methods protocol: Analysis of nuclear RNA interference...
09 September 2015 7,214 3 View
What is known about the effect of different thoraco-abdominal breathing pattern on (regional) lung perfusion or lung ventilation in healthy adults? Does it make a difference if I breath more with...
01 September 2015 6,437 4 View
How do you mature DCs isolated from splenocytes? How long should they be pulsed?.. I am looking in literature but there are several contradictory protocols, and would be nice to hear from somebody...
25 August 2015 3,076 3 View
Hello~ I am trying to transfect GFP-N1-5-HT1a into N2a cells using lipofectamine 3000. Before, I tried GFP-C1-5-HT1a with the same experimental condition, and the transfection efficiency can be...
12 August 2015 6,182 6 View
Hi all. I have performed an MTT cell viability assay and the vehicle controls do not seem to be metabolizing the MTT reagent. I am basing this on the fact that I definitely see live adherent cells...
08 August 2015 6,681 7 View
I'm interested to know if there are any well known markers to study NETosis in neutrophils using IF?
08 August 2015 9,667 5 View
I am experiencing very low O.D values in MTT assay, when I measured using microplate reader at 570 nm, even though I took enough no of cells there is no much increase in O.D. is it quite common or...
08 August 2015 3,111 13 View
Ive noticed that when im using the biorad mini system for western blot there tends to be a small amount of overflow into adjoining wells when im loading the samples even though im loading often...
08 August 2015 6,177 5 View
Hi, I'm currently setting up T-DC co-cultures and am looking for advice. T cell line is IL2 dependent. T cell media contains IL2 (amongst other supplements), although DCs produce IL2. Any advice...
08 August 2015 7,136 11 View
What are the methods to study cell proliferation kinetics ?
08 August 2015 10,087 6 View
A eukaryotic protein is predicted to be secreted by the SignalP 4.0. And how can I determine wether the protein is indeed secreted or not by the experiment? Is there any simple assay can be done...
08 August 2015 8,867 6 View
Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When PI is bound to nucleic acids, the fluorescence...
08 August 2015 5,782 12 View
I am working with macrophage cells and time is of the essence with what I am trying to do. I know that inflammasome formation requires "two hits" of some type of immune stimuli in order to form...
07 August 2015 1,226 12 View
I am treating AR42J cells with 10-8 M cholecystokinin (CCK) for different time intervals up to 8 hours. Following this, I need to check for markers of cell death and for activation of the Unfolded...
14 July 2015 8,452 3 View
I know that in human cell-lines, some of the N-linked sugars that are added to proteins during maturation in the secretory pathway contain sialic-acid residues. This gives the N-linked sugars a...
07 July 2015 753 4 View
07 July 2015 7,535 4 View
Hello, I try to establish a 107 a assay unfortunately without success. I used human PBMCs and cocultured them with several cancer cells (with and without developed drugs). The flow results looked...
07 July 2015 1,321 1 View
I'm curious as to how to terminate protein expression in T7 Express Competent E. coli (high efficiency) after gene induction with 0.2% arabinose? Is there any way to halt this besides waiting for...
07 July 2015 4,512 3 View
There is some evidence that the absolute difference in PI or PSV is not significant.
14 June 2015 4,062 2 View