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Questions related to Cellular Biology Techniques
I am wondering if I should fix and permeabilize my cells with a 50:50 mixture of ice cold methanol and acetone when I perform transwell migration assays. I have seen most methods mention using...
14 June 2017 8,535 2 View
Dear All I intend to use mitosis index, using DAPI staining, to measure synchrony level of plant cell suspension after treatments. Size of aggregates in the cell suspension are less than 500...
12 June 2017 6,628 11 View
I need to study the SOD level in granulosa cells of the cumulus-oocyte complex. Can any experts tell me how to lyse the cells and count for 5 million cells/vial?
11 June 2017 3,986 3 View
Respected members My junior want to start work on Balsam in respect of gamma irradiation. So please help us
19 May 2017 1,933 3 View
I treat arabidopsis seedlings in liquid culture with flagellin and want to track pH changes over 40 min by taking aliquots of 100 ul and measuring pH. Any other idea to do so is appriciated. Thanks
18 May 2017 9,586 1 View
I am presently using Freeze thaw method, giving only 5-10% . Suggest a better method.
07 May 2017 2,964 3 View
I am trying to perform colonogenic assay on MDA-MB-231 cells. After 14 days of treatment, I wash cells with PBS and add 0.1% crystal violet in methanol to the cells. I stain them for 30 minutes in...
07 May 2017 3,202 3 View
Hi Guys, I cannot find anything in literature. I have olny found a paper in yeast.. I see that K63 ubiquitination decreases upon mg132 treatment. I would have expected an increase...any cues? Tnx...
04 May 2017 889 3 View
I am working on a project where I have an image with a group of cells and I need to count them.
30 April 2017 4,196 5 View
I am currently working on DLBCL cell lines, and trying to isolate RNA from exosomes of cell lines. I isolated the exosomes from cell lines using the standard protocol of ultracentrifugation and...
17 April 2017 7,294 3 View
Good day all, Whats the mechanism by which the Nuclear Matrix Protein 4 can control the UPR? Thanks
17 April 2017 2,619 2 View
The patient with uruptured aneurysm. Planned cardiac surgery for replacement aortic valve.
13 April 2017 876 3 View
The cells were previously thawed 4/7 and then seeded at 0.4e6/mL until 4/10 then the count was 1.5e6/mL. Diluted them to 1e6/mL and grew overnight. Today the count is still at 1e6/mL. Why didn't...
11 April 2017 5,877 1 View
Could someone kindly explain to me why some protocol do not wash the cells after staining with FITC-Annexin V and PI, to get rid of unbound annexin V/PI ?
30 March 2017 3,683 2 View
We use the BrdU ELISA Kit from Roche a lot & never had any problems with it. Somehow, now we observe crystal formation (dark violett to black crystals) in the last steps of the assay... we did...
27 March 2017 3,895 2 View
Hi, I am performing UV irradiation studies on human fibroblasts. I want to perform IF after UV exposure and a short recovery time, which would follow pre - extraction at 4 degrees and PFA 4%...
21 March 2017 3,761 1 View
Annexin/PI for attached cell lines
16 March 2017 5,550 2 View
Does anyone works with or has came across any research article describing the tumor cells which primarily depends on anti-apoptotic/pro-survival protein Bcl-XL for their survival? From Bcl-XL...
03 March 2017 1,565 3 View
Hello all, I want to use Ki67 along with IFN, TNF in relation to T cell inhibitors like PD-1. If I use PBMC's and stim with PMA/iono for 6 hours with golgi inhibitors I am unable to see Ki67...
28 February 2017 6,387 4 View
Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...
22 February 2017 918 2 View
Hi, everyone! Recently I'm collecting lentivirus. After package in 293T cells, I take the medium, centrifuge at 1000rpm for 5 min to pellet the cells, then take the supernatant and filter by...
19 February 2017 7,408 5 View
I had to work with HeLa cells, and I have a question related to cell culture work. What would happen if I wash HeLa cells with water? I am asking this as when i wash the cells with water, all...
03 February 2017 10,048 4 View
I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP) Any suggestion on how to reduce? Thank you!
31 January 2017 6,231 1 View
Pictures taken at 200x
27 January 2017 3,242 7 View