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Questions related to Cellular Biology Techniques
Good day all, Whats the mechanism by which the Nuclear Matrix Protein 4 can control the UPR? Thanks
17 April 2017 2,502 2 View
The patient with uruptured aneurysm. Planned cardiac surgery for replacement aortic valve.
13 April 2017 755 3 View
The cells were previously thawed 4/7 and then seeded at 0.4e6/mL until 4/10 then the count was 1.5e6/mL. Diluted them to 1e6/mL and grew overnight. Today the count is still at 1e6/mL. Why didn't...
11 April 2017 5,785 1 View
Could someone kindly explain to me why some protocol do not wash the cells after staining with FITC-Annexin V and PI, to get rid of unbound annexin V/PI ?
30 March 2017 3,583 2 View
We use the BrdU ELISA Kit from Roche a lot & never had any problems with it. Somehow, now we observe crystal formation (dark violett to black crystals) in the last steps of the assay... we did...
27 March 2017 3,802 2 View
Hi, I am performing UV irradiation studies on human fibroblasts. I want to perform IF after UV exposure and a short recovery time, which would follow pre - extraction at 4 degrees and PFA 4%...
21 March 2017 3,667 1 View
Annexin/PI for attached cell lines
16 March 2017 5,453 2 View
Hello all, I want to use Ki67 along with IFN, TNF in relation to T cell inhibitors like PD-1. If I use PBMC's and stim with PMA/iono for 6 hours with golgi inhibitors I am unable to see Ki67...
28 February 2017 6,181 4 View
Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...
22 February 2017 715 2 View
Hi, everyone! Recently I'm collecting lentivirus. After package in 293T cells, I take the medium, centrifuge at 1000rpm for 5 min to pellet the cells, then take the supernatant and filter by...
19 February 2017 7,320 5 View
I had to work with HeLa cells, and I have a question related to cell culture work. What would happen if I wash HeLa cells with water? I am asking this as when i wash the cells with water, all...
03 February 2017 9,944 4 View
I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP) Any suggestion on how to reduce? Thank you!
31 January 2017 6,120 1 View
Pictures taken at 200x
27 January 2017 3,102 7 View
For the qunatitaive study of atherosclerosis in mice, is gelatin embedding of heart important or can we do snap freeze with isopentane? My understanding is that, to study atherosclerosis, we need...
27 January 2017 5,802 1 View
Hey, I do have a question with murine GM-CSF and M-CSF for making rat BMDCs and BMDMs? I want to use WEHI 3B and L929 conditioned cell culture media in order to generate rat BMDCs and BMDMs. Is...
24 January 2017 2,696 3 View
Cell viability assayHepG2 and Huh-7 cells and primary human hepatocyteswere seeded in 96-well plates at 1 × 104 cells per welland cultured for 24 h. The cells were subsequentlystarved in culture...
24 January 2017 410 10 View
Hello, I have soft-tissue samples from different organs stored at -80 (previously snap-frozen in liquid nitrogen). I would need to isolate cell nuclei and fix on slides and I need the nucleus...
21 January 2017 8,103 6 View
I am making hypoxia situation in human pigment epithelial (RPE)using CoCl2. I read the some paper that the cobalt could make hypoxia situation and reduce the cell viability. however i used from...
18 January 2017 5,186 4 View
Is 4 hours too long? Would the MFI signal decrease by more than 50% of the expected value?
18 January 2017 3,188 4 View
After centrifuging in a v-bottom 96-well, at 2000rpm (800G), 3min, 4deg, (volume about 180ul) I added 180ul buffer without breaking up the pellet, and then centrifuged the cells again. (i.e i...
18 January 2017 1,015 1 View
Im working on the targeting of NPs to specific tumor cell and to relase the payloads there.
25 August 2016 8,971 3 View
Dear All, I observed a 58 yo female patient in deep pain and was diagnosed as having a left-sided atypical chest pain. And she was sent for a chest X-Ray. It was found that she has a 1.0 cm x 0.8...
10 June 2016 9,736 6 View
Hi, I need to purchase non small cell lung cancer (NSCLC) fresh tissues and matched blood. I am looking for a company in Europe (preferably in Germany). Moreover I would like to know which is the...
07 June 2016 9,016 8 View
Can the use of different types of mechanical ventilatory modes cause peripheral vasoconstriction? During the cares of a patient on BIPAP ASB it was noted that the patients fingers were both cold...
15 May 2016 8,252 3 View