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Questions related to Cellular Biology Techniques
Hi! We have been discussing which volume is optimal for culturing spheroids in 96 well plates. Usually when culturing monolayers or immune cells, I use 200 uL/well in order to have sufficient...
28 March 2025 1,527 2 View
i am working on Pulmonary Hypertension, and currently interested in mutant vascular smooth muscle cells' role in the progression of this disease, but I am wondering How can in vitro models be...
31 January 2025 9,850 0 View
My downstream experiments require high protein concentrations from various cell lines, mostly, breast cancer cell lines. Here's my approach: Briefly, I prepared 15 cm plates with 90–95% cell...
16 January 2025 8,615 5 View
Hi everyone, I am working on in-vitro transcription to generate mRNA for LNP fabrication. The kit I am using is the HiScribe® T7 mRNA Kit with CleanCap® Reagent AG from NEB. The first time I was...
15 January 2025 3,000 6 View
Hi all I detected one molecule for my paper. However, my band is faint/negative band to show. Please, give me the comment for detecting this protein ? I wrote my protocol for WB 1. Protein...
31 August 2024 8,357 0 View
I have truck tire particles that I am using in my column experiment. I am trying to do size determination using the Malvern Zetasizer. My sample concentration is 0.1 mg/L, i.e., 10 g of sample was...
23 June 2024 8,912 6 View
Hello fellow scholars, I would like to know if I measure the drug release of my liposome through a dialysis membrane, how to solve the model that this device may cause [two-stage release]? Of...
10 April 2024 442 0 View
Hi. I am looking for the excitation-emission matrix of a compound called 5,6-dihydroxiindole (DHI). I irradiated tyrosine with UV and want to see if the emission peaks I got match with the ones...
10 December 2023 3,526 0 View
Type? (other than prophylactic LWMH) Duration?
01 September 2023 7,685 1 View
Is there a connection between stadialism and Hegel and his intellectual heirs? Who can be called the father of stadialism? Is it interwoven with British empiricism?
25 August 2023 670 1 View
In western blot, getting a thick cleaved band of gasD protein in the control sample. As samples are healthy subject then what will be the cause of getting cleaved band in untreated sample?
27 May 2023 9,474 0 View
In the experiment of inducing pyroptosis of THP1 cells, when adding ATP after pretreatment of cells with LPS, is it necessary to remove the LPS and then add the medium containing ATP, or add ATP...
08 May 2023 1,991 1 View
I need journals andbooks on the topic
04 March 2023 4,717 2 View
We are trying to reproduce a model of MLRP3 activation. Based on literature protocols, we prime peritoneal murine C57bl/6j macrophages with 1 ug/ml LPS in DMEM or RPMI1640 for 3 hr., then incubate...
14 October 2022 9,615 1 View
Hi, I want to isolate slow growing clones from a cancer cell line using LDC method. Anyone can help me in this, it would be a great help for me. I do not have cell sorter and the cell line is...
13 February 2022 8,665 0 View
Does anyone have experience doing scratch assays in stem cells in 96 well plate using the wound maker? I notice that after doing the scratch and washing twice, the cells round up and there are...
26 January 2021 7,455 3 View
During initial stages of transradial arterial route for interventions : which is better single wall or double wall puncture
06 November 2020 6,981 1 View
Manual homogenization using glass homogenizer takes lot of time, difficult to handle 50-100 tissue samples per day. Issue with mechanical homogenizer is it can destroy the fungal cells too.
28 September 2020 9,939 0 View
Chiumello et al (2006) suggested that thorax and pelvic supports should not be used in prone patients lying on air mattress beds in the ICU. The supports did not significantly affect...
01 April 2020 5,968 6 View
Dear community, I am looking for whole-body MR angiography images. I would be very grateful if someone could recommend me any resource, or would like to establish a data sharing agreement. I am...
31 March 2020 993 2 View
Hi, Has anyone used 2F Fogarty Arterial Catheter from Edwards lifescience recently? I had used this product for rat carotid artery injury for more than 15 years and never had problems until...
20 February 2020 7,907 1 View
Hello, I´m trying to understand how Alexa-647 ATP can be used as a detector of intracellular mitochondria-produced ATP? Not much info in the datasheet. Briefly, ATP was marked with this conjugate...
18 February 2020 2,283 1 View
Dear colleagues, currently I am doing an inflammasome-related experiment using cells. I have a substance as a candidate for anti-inflammasome. However, I am a little bit confused about which step...
15 November 2019 2,727 3 View
We knew that inflammasome event induced by LPS and extracellular ATP could lead to the cell pyroptosis and death, but what about the effect of only ATP administration to the cell culture? Is it...
20 September 2019 3,932 2 View