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Questions related to Cellular Biology Techniques
Hello All, I would like to ask if anyone know if measuring mitochondria number in cells could be feasible by using STORM microscopy to detect a specific fluorescent mitochondrial marker. If yes is...
11 November 2015 499 5 View
I am looking to conduct transport studies in CaCo-2 cells using three fluorescent dextrans namely, Fluorescein Salt (MW~3.7KD), FD4 (MW~ 3-5KD) and FD20S (MW~20KD). All three of them are from...
11 November 2015 4,271 4 View
I will load the cell free cholesterol, then I need extract the cholesterol from the cell. With WAKO kit to do lipid assay.
11 November 2015 4,818 6 View
Hi, I have isolated some mouse splenocyte and collected the pellets after isolation. I added QIAZOL into the cell pellet, but I forgot to pipette it up-and-down before I put it into the -80...
11 November 2015 499 8 View
I have constructed one mutant having kanamycin cassette. For the growth comparison between wild type and mutant of same species, is it essential to add kanamycin antibiotic in liquid medium? In...
11 November 2015 3,934 2 View
Cells where treated with Bafilo alone without any stresser or autophagy inducer
11 November 2015 4,809 6 View
Hi, I have a Sutter Puller P-97 and I am looking for protocols to pull glass capillaries (final tip diameter > 20 um) for injecting cells into tissues. Could somebody advice on which parameters...
11 November 2015 6,514 3 View
How can I carry out a 7 day cell-based proliferation assay? Is continuously refreshing the medium necessary, or should I just place it waiting for detection?
11 November 2015 4,433 19 View
What criteria do you use to surgery? Which embolization technique do you use?
09 November 2015 5,934 5 View
Hello everyone, Does anyone know how I can isolate and transfer a spheroid from one plate to another one? how can I wash a spheroid with PBS (e.g. Can i use centrifugation method? and how can...
27 October 2015 771 7 View
My friend asks: We have to block protein transport in vessel tissue culture, is it reasonable to incubate small pieces of tissue in collagenase/DNAse cocktail with Brefeldin A for about 1,5h...
10 October 2015 3,069 1 View
I am looking at PAR expression in our particular cell line. Unfortunately though, Trypsin, which is generally used for cell for cell detachment during passaging etc. is well known to activated...
10 October 2015 9,025 2 View
Hello Guys, Vortexing cell suspension for short time (~10sec) to homoginize solution before distribute cell into vessels. Can this damage the cell? Thank you Cheerio,
10 October 2015 4,458 5 View
Hello everyone, I am trying to get rid of hematopoietic lineage in bone marrow using the lineage depletion kit from Miltenyi. Without depletion, CD45-/Ter119- cells comprimise 8-10% of the whole...
10 October 2015 7,454 1 View
Using either MDCK, ARPE-19, or pig RPE cells.
10 October 2015 5,566 5 View
Looking for AAV-mediated expression of these opsins (see link)
10 October 2015 6,996 4 View
I tried to apply 3C experiment in NIH/3T3 cell line. NIH/3T3 is a Mouse Embryonic Fibroblast cell line. 1*10^7 cells were used for 3C experiment. However, by adding 0.3% sds in 1* NEB buffer 2.1,...
10 October 2015 6,838 3 View
1) Acetaldehyde and acrolein both are very evaporable and there are some loss during the solution preparation and incubation with cell in the 96-well plates or other plates. So A549 cell...
10 October 2015 3,806 3 View
We know that,we use stents to splint or keep the coronary artery open.There is several types of stent procedure.So,when we use nitinol stents,and what are its advantages over others?
08 October 2015 2,018 3 View
With the resistance value of coronary artery in hand is there any relation or formula to find the pressure pertaining at coronary artery?
21 September 2015 391 1 View
Regarding its pore size ,brand name catalog number?
15 September 2015 7,231 2 View
Their molecular structures are quite similar, but I have really no idea about such replacement...
10 September 2015 2,788 3 View
HI everyone, Could I have your opinion on these photos please ? I revived my HEK293T cells yesterday in FreeStyle medium and I noticed today those black floating vesicles. I had contamination...
09 September 2015 777 4 View
I am looking for a method to totally suppress RNA transcriptase in cell suspensions and see if any new strands of RNA are still elaborated as measured by uptake of tritiated nucleotides.
09 September 2015 6,812 6 View