Contact experts in Cellular Biology Techniques to get answers
1,528 views 395 posts
Questions related to Cellular Biology Techniques
I want to use, 1:10 MOI, (cell: bacteria). Assuming that one well of cell is 1 x 10^5, thus, the no of bacteria per well should be 10 x 10^5. The bacteria is V. cholerae, and from my reading, at...
06 June 2015 757 1 View
Does anyone have experience with the CellROX Green reagent for oxidative stress detection? We are trying to detect oxidative stress in cells(live or fixed) in 35mm plastic dish. Unexpectedly, the...
06 June 2015 8,724 13 View
Hi! I am trying to reversibly inhibit DNA replication of my mouse cells. I read that a lot of inhibitors actually cause DNA crosslinking and eventually lead to apoptosis, which I don't want. I am...
06 June 2015 5,080 9 View
I use regular KGluc solution, but with ajusted to 450 mOsm osmolarity. Bath solution is regular Tyrode-based, with normal osmolarity. Under these conditions gigaseal forms very quickly, but when I...
06 June 2015 6,749 4 View
I found a paper (ref below) that describes uptake of ADP-ribose (3H-labelled) in erythrocytes. I was wondering if anyone has any experience with this protocol or has looked at ADP-ribose uptake in...
06 June 2015 8,066 1 View
The resistance reading from WPI EVOM2 is ohm x cm2 or just ohm? I read the manual very carefully and thought it's ohm x cm2 and we don't need to convert it. But the TER value I got for my RPE...
06 June 2015 5,033 2 View
I am a student researcher, investigating whether glibenclamide can restore insulin secretion in cells with Wolfram syndrome (WFS1 knockdown). The team I am working with have been gifted some MIN6...
06 June 2015 1,974 3 View
I've been using the silicon oil filtration method from the Heldt papers, which uses tritiated water and tritiated sorbitol to measure intracellular volume. The scintillation counter reads...
06 June 2015 6,017 0 View
To calculate growth index, I am using the below formula for Dry weight of the cells. W (final)- W(initial)/ W(initial) However, the resulting number is high almost higher than five. I was...
06 June 2015 3,936 1 View
Hi, I'm currently trying to detect TNF via IF in mouse leukocytes (specifically neutrophils but will take what I can get at the moment). I have tried a range of positive controls (splenocytes...
06 June 2015 4,554 3 View
it is well known that mutations in some genes can leads to cancer condition. if in that case how those mutated gene products can able to participate in different pathways? especially in the case...
06 June 2015 4,563 4 View
Searching the web i found that Zymo Research makes a kit for making competent cells and transforming them in aprox 20 seconds without incubating the mix on ice for 30 minutes, without heat-shock...
05 May 2015 3,543 4 View
Currently, I am trying to stain muscle frozen section with three antibodies CD31, PAX7 and laminin. I have tried fixing them using ice-cold 100% methanol and 4% formaldehyde. block for 45 min and...
05 May 2015 5,848 14 View
I'm interested in the functional impacts of Golgi fragmentation - what are good recent articles to read that discuss Golgi structure/function?
05 May 2015 4,660 3 View
Hey everyone! i am trying to analyze the effect of a drug on mammalian cells. I know that I have to starve the cells before treatment but someone told me that I have to starve the cells again...
05 May 2015 7,847 5 View
I have used electroforamation method to prepare the gaint unilamellar vesicle. kept the liposome solution overnight for dialysis using the dialysis tubing. I am not getting what is the problem...
05 May 2015 278 19 View
I came across this trypanosome specific phosphodiesterase inhibitor CpdA in few papers. However I could not find any company which sell the same. Any leads?
05 May 2015 497 1 View
Reading single cell papers made me wonder why researchers use 100 cells, 500 cells, or 1000 cells? What's criterion to call such numbers? How do you know you got the whole picture by using such a...
24 April 2015 2,438 6 View
Hi, I am using DAPI for screening the nucleus in a tobacco cell line. However, when looking at the cells under microscope, DAPI is visualized mostly in the membrane and somehow in cytoplasm other...
24 April 2015 643 5 View
The dynabead protocol suggests to use at least 100ul of antigen-containing lysate to resuspend the bead in immunoprecipitation. I usually 10ug of lysate dilluted in PBS to get 100ul and give me...
24 April 2015 5,253 5 View
Hi, I'm currently running DNA (300 bp) on 8% acrylamide gels for heteroduplex analysis. I loaded samples 2 times on the same gel (I loaded the first samples and let it run for 4 hrs and then I...
17 April 2015 3,455 11 View
I hope you can help me thanks
17 April 2015 6,093 3 View
I have subjected pieces of explanted liver samples for collagenase treatment (1/2 an hour at 37), and purified through Percoll centrifugation. However, the cells I obtained do not look polygonal,...
10 April 2015 2,324 3 View
I like Human Hek293 cells because they transfect so well - usually ~95% with Lipofectamine. However I need to work with a Rat cell line to test CRISPR gRNAs in and having a rat cell line that...
09 April 2015 5,932 11 View