What are the possible ways of basolateral-to-apical signal transitions for polarized epithelial cells in culture?
I suppose, cytoskeleton is one of the sensing mechanisms, with basolateral anchoring influencing cell shape and behaviour at the apical side.
However, I am now looking for the mechanism regulating protein phosphorylation, which is probably different from the cytoskeleton arrangement.
Particularly, in my experiment changing basolateral conditions result in variations of apical protein activity, which is most likely regulated by phosphorylation. What are the possible basolateral signals and/or sensors, influencing protein kinase activity towards apical proteins and mechanisms underlying these effects? How is protein kinase targeted to the apical cell side?
Many thanks
While doing my usual ToC scanning, I saw a publication very recently (last 2-3 weeks) on exactly this topic in a high impact factor journal - one of the Cell or Nature journals?? I will try to look back and post the reference if I can find it; however, I imagine that a key word search in PubMed e.g. using 'basolateral to apical' or some such combination would very quickly find it.
I hope that helps!
The following is NOT the reference I was thinking of - I will keep looking - but I hope that it may help:
Distinct apical and basolateral mechanisms drive planar cell polarity-dependent convergent extension of the mouse neural plate.
Williams M, Yen W, Lu X, Sutherland A.
Dev Cell. 2014 Apr 14;29(1):34-46. doi: 10.1016/j.devcel.2014.02.007.
See also the following and please up-vote my answers if you find them to be useful:
Emergence of an Apical Epithelial Cell Surface In Vivo
http://www.sciencedirect.com/science/article/pii/S1534580715007984
Dear Dr.Morley!
Thank you very much for your interest, time and help.
Unfortunately, I still couldn't find the paper you were talking about, but I ran through several articles that were new for me and, as I hope, will be useful.
Also many thanks for the links, I am going to read right now.
Your answers are indeed very useful so the up'vote is not a question.
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