Hello

I suppose that you produced a specific protein in Saccharomyces cerevisiae yeast cells and want to inactivate the cells before the obtention of the protein. Conventionally, you could try an inactivation with hot water or steam, but maybe you're concerned about the possibility of a thermodegradation of the protein. Perhaps you could check this information:

https://centec-usa.com/inactivation-of-yeast/#:~:text=Hot%20water%20or%20steam%20can,live%20cell%20membranes%20are%20ruptured.

Article Effective inactivation of Saccharomyces cerevisiae in minima...

I hope I've helped.

Thanks for your reply@ Raziel. Hot water or steam can damage my protein structure and stability. Is there any chemical or UV treatment use for inactivation.

with regards,

Dipak Das

Maybe you could try to use freeze-drying to inactivate the yeast cells, thus removing all the water. This method does not cause thermal degradation related to thermal-based methods like hot water or steam.