I have done sugar analysis for several times using thin layer chromotagrahy but can't get the spots on plate for both sugar standards and samples. We use ethyl acetate-pyridine-water for mobile phase. For dye, we use aniline phytalate. All the materials that we use are in good quality. We were careful during the study that we spotted the samples above 2 cm above the bottom of the plate. After dye and incubation at 105 celcius degree for 5 minutes, we get almost an emty plate without no spots. Do you have any suggestions?
When performing thin layer chromatography (TLC) for sugar analysis, obtaining well-defined spots is crucial. Here are some common reasons why you might not see spots on the TLC plate:
It sounds like you’re experiencing challenges with your thin-layer chromatography (TLC) analysis for sugar separation. Let’s troubleshoot this together! Here are some suggestions:
Dear Panchal,
Thank you for your detailed answer.
We have already taken into consideration of the points you mentioned and made some changes.(incubation time-temparature, quality of silica gel plates) but with no results.
Today, we decided to change the dye with a brand new, unused one( aniline phytalate again, of course) We thought that the dye we were using for our no-result-TLCs for several days might not be in good quality for some reasons since before these unsuccesful trials, we used to always get good results with former dye. After using brand new dye, we are finally able to get good results. So now we'll continue with this dye.
Thank you for your attention,
Emre.
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