Hello Beatriz, your question is not quite clear, but I try to give you an explanation. If you do not likt to embed your tissue in paraffin or other resins you have to cut it on a cryostate or freezing microtom. You can also use a vibratome but then you need a stronger fixation, min. 4% PFA.

If you like to cut unfixed tissue then you have to use a cryostate. The cryostate has a cooled chamber which leads to additionally knife cooling which is essential for unfixed or mild fixed tissue. If you are working with embedded tissue then you should use a rotating or sliding microtome. The advantage is that you can cut much thinner sections, 3 - 10 µm. With all techniques you can do IHC but it depneds of you antibodies which technique you should prefer. Check the data sheets. They will give you informations of fixation and suitability of the kind of sectioning. Good luck!