What is a common and simple functional analysis for testing the potency of a cancer stem cell?

I have tested my glioblastoma U87 cancer cell lines for cancer stem cell markers. I wish to see a way other than stem cell marker expression, the potency of my cells.

31 December 2015 6,934 3 View

What is a simple technique to measure cologenicity in cancer stem cells?

I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...

31 December 2015 9,407 2 View

How do you separate quiescent cells from senescent cells?

11 December 2015 7,410 5 View

Are cancer stem cells also the metastatic/invasive cancer cells?

I am trying to understand whether cancer stem cells are the metastatic cells in cancer progression because of their ability to self-renew and proliferate on a single cell level.

09 October 2015 4,298 0 View

Is N2 or B27 supplement better for culturing Glioblastoma Multiforme Cell line (U-87)?

I am culturing this U87 cell line and I was wondering what is the recommended media. I have read the literature, but they have been giving conflicting answers. Also, if there is any extra advice...

09 October 2015 9,085 3 View

How can you further differentiate gliobastoma cancer cells and neural stem cells?

I am try to find a way to differentiated the cancer cells to a point where they are terminally differentiated. They are similar to Neural stem cells which is why I removed all of the growth...

09 October 2015 8,364 2 View

Can you culture glioblastoma multiforme cells with neural stem cell media?

07 August 2015 9,290 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View