The best and easiest way to do that, is to add Morphine. see below:
http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=2894
Breast tumor stem cells highly express ALDH1 and CD44. Flow cytometry is useful to select the population of ALDH1(high)/CD44(high)/CD24(low) in breast cancer cells as a whole.
Clinically, the iatrogenic increase of CSCs has attracted much attention in the clinical settings. The minimal residual disease (MRD) tend to be enriched in CSCs which are capable of surviving and proliferating under the selective pressure of oxidative stress.
http://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0221-y
http://onlinelibrary.wiley.com/doi/10.1111/cas.12817/abstract
Dr. Yoshida has correctly mentioned about the biomarker to enrich BCSCs, however there are other biomarkers as well like CD44+/CD44- , Side population assay and Mammosphere assay. I think CSCs should be isolated on the basis of its functional assays.
Thanks
HI Zahra,
We have a new method to identify and sort CSC from tumors:
https://www.researchgate.net/publication/275662555_MRNA_detection_in_living_cells_A_next_generation_cancer_stem_cell_identification_technique
Kind regards,
Steve
Article MRNA detection in living cells: A next generation cancer ste...
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