Hello everyone,
I am planning to assess the effect of a drug on cancer stem cells proliferation. Giving that the mammosphere assay is an efficient way to assess cancer stem cells proliferation, I woud like to know which method is experimetally appropriate: treating adherent cells with the drug and then using surviving cells to generate mammospheres or generate mammospheres from non treated cells and then treat mammospheres with the drug.
I would be really grateful for sharing opinions and experiences.
Hi Hamadi,
in your first experimant you would evaluate the capacity of your drug to inhibit formation of mammospheres. The second one would evaluate the proliferation of your cells inj mammospheres under the influence of your drug. I suggest the second experiment, whikle the outcome of the first one may be intersting to you too.
Hope that helps.
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