According to bionumbers, anywhere between 18h and 72h. There is probably a lot of variation in cell cycle times from one division to another, and more between different cells. The circadian clock can gate the cell cycle and synchronize them, at least partially at periods close to 24 or 48 h.
https://www.researchgate.net/publication/276458632_Coupling_between_the_Circadian_Clock_and_Cell_Cycle_Oscillators_Implication_for_Healthy_Cells_and_Malignant_Growth
http://bionumbers.hms.harvard.edu//bionumber.aspx?id=100685&ver=16
Article Coupling between the Circadian Clock and Cell Cycle Oscillat...
The problem in answering your question is "How does one define the CSCs?" Assays that use cell surface markers to define a so-called phenotype identify a much larger population than the CSCs, and hence, answers based on such data are invalid. (See Kern & Shibata; J. Hill; Lange et al.). Those using a functional definition (Till & McCuloch), as in the Hybrid Spheroid Assay (Lange et al.), can provide reasonable answers, e.g., 22 -24 hrs as the CSC doubling time, but the cycle time may be a bit shorter as there may be a slowing due to the rate of new niche site production and for the extruded daughter to find a vacant niche.
HI Angel,
We sort CSC from tumors based on true stem markers such as Nanog and Sox2
https://www.researchgate.net/publication/275662555_MRNA_detection_in_living_cells_A_next_generation_cancer_stem_cell_identification_techniquee
Once sorted to 100% purity we put these primary CSC back into cultrue in low oxygen conditions (3%) and observe doubling times of 16-24 hours of the cells are seeded light. More dense cultures seem to show the slower phenotype and cycle in the 36-48 hour range.
Kind regards,
Steve
Article MRNA detection in living cells: A next generation cancer ste...
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