I carried out a WGS of the mitochondria DNA in cancer and its corresponding adjacent tissues. The analysis show two significant SNPs with a very high OR of exposure to cancer. Consequently, I would like to determine the functional contribution of these mutations(insertions) to the carcinogenesis in a cancer cell line. I would like to know if there is an appropriate method to replicate this insertion in a cancer cell line.
regards
Hello Justice,
Technically this could be done with CRISPR/Cas9 gene editing, employing the HDR pathway. But it may not be easy.
This may help:
Article Knock-in strategy for editing human and zebrafish mitochondr...
Article Efficient mitochondrial genome editing by CRISPR/Cas9
Article Mitochondrial Genome Engineering: The Revolution May Not Be ...
Good luck!
Thank you i will check, out i asked a CRISPR cas9 company and they said when they attempted that the resultant cell was a heterozygote.
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