What would be the key difficulties found in expressing a membrane protein from gram negative bacteria in gram positive bacteria?

02 February 2014 0 2K Report

What would be the key points to be addressed while expressing a membrane protein from gram negative(E.coli) in gram positive clostridium acetobutylicum?

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Trying to amplify a gene from genomic DNA with primers with low GC content and attached restriction sites, what would be the optimum PCR conditions?

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