Can anyone help with chromogenic/fluorogenic substrates for pectinases?

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Vijay Sonawane

Hello Gustavo,

I would like to know if you need this substrate for a purpose other than screening.

For screening ,Iodine staining or Ruthenium statining is quite effective method.

Regards,

Gustavo Orlando Bonilla-Rodriguez

HI Vijay, I'm looking for synthetic substrates for experiments of kinetics.

I have no idea if ready made substrate is available for estimation of alpha-glucoronidase .activity.

However, I think you can explore calcoflour binding with pectin? I wonder if it binds with pectin. It binds well with cellulose and chitin.

If the calcoflour binds with pectin, then a quantitative assay for pectinase could be developed.

Regards

Vijay, thank you for your suggestion!