Date palm micropropagation
I crossed two lines of rice with contrasting leaf, culm and panicle traits. The F1 plants were almost halfway between the parents except for some quantitative heterosis. The F2 plants (currently...
08 September 2018 7,867 8 View
I applied urea and iron EDTA and then after a few days my rice plants were like that. It appears that this is iron deficiency but I have just applied iron. What could be the reason of young leaf...
05 June 2018 4,658 6 View
On 24 August 2017, I submitted a manuscript to A JOURNAL WITH AN IMPACT FACTOR OF MORE THAN 2.0. Four months later, the manuscript status was under review. I felt this was enough for the journal...
02 March 2018 6,814 1 View
I am thinking of buying an HPLC system. Could I have your suggestions on: 1- Which is better, an integrated or customized system? 2- which specifications of the pump, columns will give broad...
09 October 2016 6,711 8 View
I do not speak Spanish or Japanese and defenitley many researchers do not speak arabic. Is it acceptable and sensible to cite a paper that was originally published in a language other than...
08 September 2015 7,892 3 View
Does any colleague have pTOK233 or pSB134 and want to share?
01 February 2015 5,879 1 View
When rice is grown in anaerobic soil, it develops barrier to radial oxygen from root to soil. This barrier is mainly suberin in the outer layers of root. Is it logical that this barrier does not...
08 September 2014 1,792 5 View
To quantify expression of a gene with semi-quantitative RT-PCR, the cDNA is synthesized and equalized based on an internal control gene such as 18S rRNA. The gene of interest is then amplified...
02 March 2014 589 3 View
Hydraulic conductance
01 February 2014 4,591 20 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
