How many generations of hybrid rice should I grow to obtain true breeding lines?

I crossed two lines of rice with contrasting leaf, culm and panicle traits. The F1 plants were almost halfway between the parents except for some quantitative heterosis. The F2 plants (currently...

08 September 2018 7,703 8 View

What kind of deficiency is this?

I applied urea and iron EDTA and then after a few days my rice plants were like that. It appears that this is iron deficiency but I have just applied iron. What could be the reason of young leaf...

05 June 2018 4,542 6 View

How carefully or how carelessly do editors of some journals handle our manuscripts?

On 24 August 2017, I submitted a manuscript to A JOURNAL WITH AN IMPACT FACTOR OF MORE THAN 2.0. Four months later, the manuscript status was under review. I felt this was enough for the journal...

02 March 2018 6,694 1 View

How to decide on buying an HPLC system?

09 October 2016 6,549 8 View

Is it sensible to cite a paper that is published in a language other than English?

I do not speak Spanish or Japanese and defenitley many researchers do not speak arabic. Is it acceptable and sensible to cite a paper that was originally published in a language other than...

08 September 2015 7,788 3 View

Does someone have pTOK233 or pSB134?

Does any colleague have pTOK233 or pSB134 and want to share?

01 February 2015 5,766 1 View

Why does not the barrier to radial oxygen loss in rice root limit water permeation?

When rice is grown in anaerobic soil, it develops barrier to radial oxygen from root to soil. This barrier is mainly suberin in the outer layers of root. Is it logical that this barrier does not...

08 September 2014 1,667 5 View

If the same job is done with real time RT-PCR, will it be able to discriminate between products of specific reaction and other unintended products?

To quantify expression of a gene with semi-quantitative RT-PCR, the cDNA is synthesized and equalized based on an internal control gene such as 18S rRNA. The gene of interest is then amplified...

02 March 2014 472 3 View

Is it fair to consider the rate of transpiration and stomatal conductance as measures of combined root and shoot hydraulic conductance?

Hydraulic conductance

01 February 2014 4,477 20 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

No title

28 February 2021 9,936 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View