Is there any good method available to measure the amount of lactic acid produced by lactobacillus spp. in a growing medium?

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Tatik Khusniati

Good method available to measure the amount of lactic acid produced by lactobacillus spp. in a growing medium was by using HPLC method

Reshy Shaheen

thanks Tatik Khusniati

use of HPLC for large number of samples on daily basis is not economical for me thats why i am looking for alternative

Dear Dr. Shaheen, Yes the use of HPLC for large number of samples on daily basis is not economical for you, so you can use alternative method, that is titration method, however this method isn't so accurate as HPLC method, but at least that method can reduce economically. In general by using the titration method , the amount of acid produced by lactobacillus spp. can be measured. By assumption that Lactobacillus spp. produced dominantly lactic acid, so the acid ampunt produced by Lactobacillus spp is estimated to be equal to the lactic acid amount produced by the same bacteria. sorry, it may be this method is a little bit help as alternative method.

Tatik Khusniati thanks a lot for your valuable suggestion

i am already trying titration its really just give the rough idea about the amount produced by bacillus spp. but not accurate enough to adopt it in standard research work. is spectroscopy helpful to estimate lactic acid by using standard solution?

Actually the accurate method for estimate lactic acid bacteria is HPLC method, However, Its better you try one sample to estimate lactic acid by using spectroscopy with standard solution and HPLC. If the result of lactic acid estimate by using spetroscopy will nearly close to that by HPLC method, you can use spectroscopy with standard solution for a lot of samples to be estimated its lactic acid. Hopefully, this matter is a little bit help to check the method as alternative method.

again thanks ma'm Tatik Khusniati

what opportunities are available at your institute for foriegn PhD students of biological sciences

The available opportunities at our institute for foreign PhD students of biological sciences are such as: (1) PhD research with one of supervisors from our institute (2) analysis of research samples by payments i

Thanks for your cooperation ma'm