Filamentous fungi electroporation.
Please suggest protocol specific for Aspergillus flavus
Prostate cancer is a prevalent form of cancer among men globally, with significant implications for healthcare systems and patient well-being. Identifying the effects of lifestyle factors on the...
15 June 2024 2,406 0 View
These biofertilizers consist of a combination of microorganisms blended in either a powder or liquid form. The accuracy of conventional methods, such as plate counting and most probable number,...
20 February 2024 7,468 3 View
Currently working with equipment-limited Cr quantification methods, specifically for concentrations of 1 to 1000 ppm, I selected a Cr(VI) quantification methodology by direct UV–visible...
24 January 2024 9,180 3 View
ns
06 December 2023 1,141 3 View
I have a 3.1 kb plasmid and I performed a digestion with the XmnI enzyme (37°C x 15 min and the enzyme was inactivated at 65°C x 20 min). When performing agarose gel electrophoresis, the size of...
21 November 2023 2,991 3 View
Hello Everyone, The scaling factors used for Collection 2 and Level 2 Landsat data includes a multiplicative (0.0000275) and an additive (-0.2) term. When these factors are used to convert to...
31 August 2023 9,941 1 View
I'm new to ELISA assays and have heard that multiplexing can be difficult. In your experience, what are some of the challenges that come with multiplexing? Is detecting up to 3 targets doable?
21 June 2023 1,373 3 View
f ¿Cuáles son los mayores factores de riesgo cardiovascular en Bailarines Profesionales?
20 June 2023 827 0 View
We're currently interpreting the results of our data gathering procedure, and these are the means and standard deviations of the scores we have gathered. I just wanted to know how do we interpret...
01 May 2023 1,530 4 View
Can you recommend me some papers about human body biomarkers preserving in soil for archeological studies?
12 December 2022 1,064 2 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View