Filamentous fungi electroporation.
Please suggest protocol specific for Aspergillus flavus
I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...
07 February 2021 7,520 6 View
I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...
10 November 2020 3,620 7 View
I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...
06 September 2020 8,959 4 View
Greetings, I'm trying to standarize TCE free staining method to visualize protein bands over SDS-PAGE, it's never been used in our lab. I've seen many published papers using this method, and the...
17 August 2020 6,620 3 View
Good morning, dear community, I have a preliminary report from an in silico analysis of non-coding short sequences against the SARS-CoV-2 virus. I'd like some advice about which indexed journals...
18 July 2020 5,365 1 View
Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...
08 July 2020 4,927 2 View
I'm trying to edit an existing citation style to suit my needs (Journal of Tropical Ecology). I edited it so the inline citations go from newest to oldest reference (Smith et al 2019, Jones et al...
15 June 2020 9,926 4 View
Hello, I am trying to look at the efficiency at which a Gene is cut after tamoximen treatment. Thus we got a set of primers that can tell us weather the Gene is cut or not. We wanted to measure...
02 February 2020 7,599 3 View
As you can see, most of our colonies express GFP while we expected to get only 50% of these or less.
04 December 2017 8,378 3 View
I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...
05 November 2017 5,899 7 View
Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1
17 August 2021 0 0 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
20 June 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help
08 May 2021 0 0 View
