Hi, i want to know can we avoid use of LA taq polymerase because of not proofreading capability for ligation? Can we use another vector which is use for proofreading taq polymerase? Please suggest me any good vector except pGEM T vector?
You need to provide more information if you want a sensible answer. In the case of conventional ligation, any blunt-ended vector will do (but you can add A-overhangs to the PCR product with Taq and dNTP, so T-vectors are also a possibility).
@Alejandro i used pGEM T vecor and LA taq polymerase for PCR, but i got no result. I want to know can i use blunt vector and pfu polymerase for pcr?
Yes, that's perfectly allright. By the way, you do not always get A overhangs when using Taq. Check PMID 8780871, for instance.
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