What method is suitable for flanking region upstream and downstream for primer binding?

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

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Has anyone used the Illumina ITS1 primer sets?

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Unknown debris sheets observed in cell culture flasks?

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Errors in DNA sequences?

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Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

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How to run repeated measures in mvabund?

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Alfonso H.Magadan

Hi Kumar,

To sequence unknown flanking regions you need to go back to old techniques like chromosome walking or inverse PCR... with the inverse PCR you design a couple of divergent primers next to a known restriction site close to your end, cut your DNA with this enzyme and then make intramolecular ligations to have circular templates. Then you amplify with the primers (now they became convergent) and sequence... I did this technique to determine the cleavage site of the CRISPR system...

Good luck,

Alfonso

Article Cleavage of phage DNA by the Streptococcus thermophilus CRIS...

Article The CRISPR/Cas bacterial immune system cleaves bacteriophage...

Manish Kumar

Thank you sir

David Langenberger

DNA Methylation Data Analysis Workshop

How to use bisulfite-treated sequencing to study DNA methylation

When?

22. - 25. November 2016

Where?

Leipzig, Germany

Link?

http://www.ecseq.com/workshops/workshop_2016-07-NGS-DNA-Methylation-Data-Analysis