There is the specific interaction between target cells and therapeutic agents. I am focussing on the determination of possible cellular targets of therapeutic peptides. I think MS and RNAseq analysis could be applied in this. Please give me suitable protocols and idea and efficacy in addressing my question.
Are there other methods to address the above question? Please suggest me and give some explanation.
Thank you.
Dear BiveK,
I would definitely recommend MS. By MS you can identify specific proteins that are highly expressed in you cancer cell lines, or even directly compare the MS spectrum between the cancer cell lines and normal cell lines. After identifying these possible peptides you would proceed with tandem MS to determine the specific sequence of each of the previously identified peptides.
Here is some relevant literature in the matter:
Protein Sequencing and Identification Using Tandem Mass Spectrometry by Michael Kinter and Nicholas E. Sherman, Wiley
Teen and Mann (2004) The ABC’s (and XYZ’s) of peptide sequencing. Nature Reviews 5:699-711.
Kellie et al. (2010) The emerging process of top down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput. Mol Biosyst. 6:1532-1539.
Hope this helps you,
Best,
Rita Pedrosa
Dear Rita,
Thank you for the rightful suggestion and relevant papers.
Is it good for the sample preparation? The solubilization of cellular proteins and determination of specific protein protein interactions using receptor molecule.
Thank you.
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