What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

Do you consider that rose bengal chloramphenicol agar is suitable to isolate and count fungus?

Someone knows about a specific law or regulation which mentions the use of the rose bengal chloramphenicol agar is suitable to isolate and count fungi. Perhaps, someone has done a research about...

07 January 2021 5,325 5 View

No title

07 January 2021 9,052 1 View

Regression analysis on small but complete dataset (perfect representation of reality)?

I have a repeated measures design with about 16 cases and 5-6 points of measuring. Sometimes, 1-4 full cases or some points of measure are missing. (The measures are 20 numerical and categorical...

20 December 2020 9,004 7 View

Approval for MnFe2O4 for industrial wastewater treatment?

I am using MnFe2O4 NPs as adsorbent for CrVI for wastewater treatment following literatures as it is one of the best, but I have a challenge of releasing Mn+2 in the environment. A lot of...

19 November 2020 3,404 4 View

FISH probe synthesis with PCR?

Hello, I have some troubles with the synthesis of a DNA probe for FISH in paraffin tissues, we decide to synthesize it by PCR with labelled dUTP, but we haven't had any results, I tried chaging...

08 September 2020 183 3 View

HAP1 cell transfection with RNP complexes?

Hi, Does anyone have experience with transfecting HAP1 cells for genome editing with Cas9/gRNA complexes? Or are the HAP1 cells difficult to transfect? Thanks.

27 August 2020 6,028 1 View

Psychologically, How can you support a Covid-19 patient ?

After being affected with that viruse , most of the patients feel depressed and sad . How can we , psychologically, encourage them ?

26 August 2020 6,178 93 View

How does COVID-19 affect the Stakeholder Engagement of YOUR EU Energy OR Marine Projects?

Dear researchers, The COVID-19 pandemic has large societal and economic impacts, from which also different stakeholders involved in EU H2020 energy projects are affected. With this survey, we...

17 June 2020 4,542 4 View

MIKE 21 Illegal Storage Access?

Has anyone ever received the error 'Illegal storage access Abnormal completion' from MIKE? I am using the Data Extractor FM and at certain times this error comes up. Once I receive this error I...

24 March 2020 2,518 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View