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Questions related to Assays
I am working on peptide containing microsuspension (particle size: 20-30 micron) which has to be sterilised, since it is a peptide molecule, facing challenges with heat sterilisation method....
01 September 2023 6,609 4 View
I want to do the antioxidant test for C.ternatea flower aqueous extract but im not sure how to prepare the sample? Should i dissolve in distilled water or methanol? There will be several...
29 August 2023 5,753 4 View
Hello everyone here I prepared and gave different concentrations of hydrogen peroxide for UV-Visible analysis. I am sending the result that I got as an attachment. Why did the shift of the peaks...
29 August 2023 6,722 0 View
Qiagen PCR purification kit is the DNA purification kit., after following the manufacturers protocol I am unable to get the yield.
29 August 2023 6,084 3 View
I am asking this question because I am working on my experiments with OVA alexa fluor 488 immune complexes and I only want these complexes to enter through the fc gamma receptors of BMDCs and not...
29 August 2023 6,025 1 View
What potential role do exosomes play in intercellular communication within the brain tumor microenvironment, and how can they be exploited for diagnostic or therapeutic purposes?
29 August 2023 4,615 2 View
Hi I am going to test some peptides as positive control for my ELISA but don't know what sort of concentration to start of with. Any suggestion will be very much appreciated.
29 August 2023 6,578 4 View
I submitted my article to a free journal that is indexed in Scopus. After an 8-month review process, I received a letter informing me that my article has been accepted for publication. However,...
23 August 2023 1,941 2 View
Hello, I need my ID on the site, but I don't understand what it is?
23 August 2023 8,315 0 View
Hello, I want to determine how much statistical power my study has in finding a significant 4-way interaction. The study uses a 2x2x2x2 mixed ANOVA design (1 between and 3 within factors all...
22 August 2023 9,298 5 View
I want to produce potassium tetrafluoroborate by treating potassium carbonate anhydrous, hydrofluoric acid, and boric acid crystal
19 August 2023 7,420 0 View
If components in an early Tertiary "sandstone" was rich in bentonite (derived from volcanics) and relatively rich in potassium feldspar grains, would a more rapid transition to illite occur at a...
19 August 2023 4,951 1 View
Most of the leaves reflect mid and far infrared radiation. But there may be few types that their outer layer is transparent to mid or far infrared.
17 August 2023 2,979 1 View
Hello everyone, I'm seeking advice on the transportation of plant extracts containing photosystem-I and II (PSI and PSII) while preserving their optical properties. Is there a specific temperature...
14 August 2023 9,489 0 View
I am working on methacylation of polysaccharides and came across a research paper that used a certain amount of methacrylic anhydride was added based on a calculation that used 5 Molar equivalent...
14 August 2023 8,140 0 View
I am a p.g student. And i want some suggestions for dpph antioxidant assay for my thesis. From the point of view as a student,
12 August 2023 6,258 2 View
I am planning to grow Oral Squamous Cell Carcinoma cells in our lab. Can someone suggest a good protocol for this? Thank you
10 August 2023 7,681 2 View
Is it possible to grow a single dish of adherent mammalian cells (e.g. HEK293) where the cells form a continuous layer but there are different media compartments? We are looking at cell:cell...
04 August 2023 2,842 1 View
In my observation, during the pandemic in 2020-21 workers from unorganised sectors learned things to survive and adopted creative ways to complete their tasks. However, people also started...
02 August 2023 2,348 0 View
I'm trying to screen for a suitable range of concentration of Gefitinib for my subsequent ELISA assay, the final aim is to select a Gefitinib concentration that does not kill cells+give off...
01 August 2023 5,197 1 View
What specific carbon source and environmental conditions have been found to be most effective in enhancing cellulase production by Trichoderma reesei, and what molecular mechanisms are believed to...
31 July 2023 2,174 1 View
How to design qPCR primers to detect the constant regions of Ig
28 July 2023 8,817 1 View
what are the functions of F1 particles in mitochondria ?
25 July 2023 305 3 View
I have performed RNA isolation from brain tissue using trizol and a commercial kit (columns). I got good RNA concentrations (>600ng/uL) and 280/260 ratios (near 2,0) in all samples. However,...
24 July 2023 203 3 View