Assays

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Questions related to Assays

Presence of comets in control samples?

Hi! I am performing an alkaline comet assay (CHO K1 cells) by setting a protocol based on several publications. I've followed every step and recommendations, but I still see comets in my control...

28 October 2022 7,863 3 View

Is there any easy way of isolating sulfur reducing/oxidizing bacteria under aerobic conditions?

In order to perform bio-desulfurization of diesel, pure cultures of sulfur bacteria have to be isolated. Can it be done in less than a month when your samples are collected from contaminated soil?

24 October 2022 8,050 0 View

How I can use sodium deoxycholate as surfactant?

I need to use sodium deoxycholate (NaDC) as surfactant in a methacrylate polymerization system for preparing hydrogel nanoparticles. But the reaction mixture (in water) precipitates before the...

23 October 2022 9,976 1 View

What is the importance of background reading in MTT viability assay?

Hi, guys. I've been doing MTT experiments for a few years. And i've always had one question about the background reading of the MTT, usually at 630nm. You can find a lot of papers in which the...

22 October 2022 8,999 3 View

How to do the c-Fos IHC staining of the PVN and ARC area of brain in mouse?

Hi! I have some trouble with IHC staining c-Fos in the PVN and ARC area of the brain. The stainnig was not able to count the positive cells. And when I decrease the background color, the green...

21 October 2022 5,745 1 View

Expression of Bacterial protein in mammalian cells ?

Suppose you are trying to express a protein in Bacteria but that protein is coming in Inclusion bodies but not secreted out. So is it possible if I express that same protein in mammalian cell with...

20 October 2022 4,295 3 View

I am repeatedly getting curves like this in my Real Time PCR. DNA samples showed bands in gel electrophoresis. Why else may I get curves like this?

I have replicated my experiments a number of times but results are coming like this all the time. I am not getting any Threshold cycle. It would be of great help if anyone could give me some...

19 October 2022 1,880 4 View

What causes an unsatisfactory correlation between substrate concentration & absorbance at 340nm in a NADH assay?

1) NH3 is generated from the substrate 2) NH3 + NADH + α-Ketoglutarate -> NAD+ + Glu In the reaction, samples with a higher substrate concentration should produce more ammonia and consume a...

18 October 2022 3,010 2 View

How do I combine two categories variables in SPSS to create one categorical variable?

Hello, I am new to SPSS and need some help. I have two categorical variables (var1 and var2) which are coded 0 - No, 1 - Yes. I am trying to assign participants to one of four groups based on...

13 October 2022 2,508 4 View

Please how do I covert the absorbance of the products of reaction involving the conversion of catechol to quinones catalyze by Polyphenol oxidase.?

I have the standard curve of the substrate which is catechol. I measured the absobance of the products (polyphenols) at 450 nm.

12 October 2022 3,940 4 View

How to use the bwwtrim function of the WRS package (robust 3-way mixed ANOVA)?

Hello everyone, in our study, we look at the effects of typical/enhanced body checking on eating pathology before and after the intervention,depending on the level of body concern (see below)....

11 October 2022 9,270 3 View

Can you give me the steps to do a dpph assay?

How can i perform the DPPH assay??. I Will use ethanol as solvent, and i will measure by spectrophotometer. I want reference paper. Thank you

11 October 2022 9,396 3 View

Buffer (medium) and cell density for red blood cell (erythrocyte) – based assays?

I’m new to red blood cell-based assays. Anyone can help me answer the following questions? Thank you very much! I plan to evaluate the effect of a compound on the hemolysis of human erythrocytes....

10 October 2022 2,512 0 View

What is the basic difference between Assay & Potency? Can assay changed after manufacturing a drug?

Suppose, Vit- E is one of the materials used to produce a capsule. while the capsule has other materials like Vit-B6, Vit-B12, ferrous sulfate, Calcium D pantothenate, Riboflavin, Nicotinamide,...

10 October 2022 8,400 2 View

Dock a ligand in a presence of a substrate on MOE?

I need to dock a possible active compound on a structure that has on the active site a co-substrate (FAD) on MOE. For now MOE just ignore the FAD and overlap the poses of the ligand on top.

07 October 2022 2,918 1 View

ReLeSR not working for picking up iPSCs?

I work with human and non-human primate iPSCs that our lab differentiates into neurons, astrocytes and microglia. We have been having an issue with the ReLeSR not picking up large clumps or...

07 October 2022 2,410 0 View

What is the best kit for micronucleus assay?

my lab wants to do a micronucleus assay.However, we do not know which kit to choose. Any opinions will be helpful.

07 October 2022 2,444 1 View

Is Distilled water act as preservative in dosage form manufacturing?

Is distilled water act as preservative?

06 October 2022 564 5 View

Anyone can recommend me a protocol to dye lysosomes in SH-SY5Y cells with LysoTracker™ Green DND-26 from ThermoFischer?

I would need a protocol to dye lysosomes wiht the LysoTracker Green DND-26 from Thermo in SH-SY5Y cell line.

23 September 2022 7,823 2 View

Is it possible to make jurkat cells memorize specific antigens and produce IFN- γ in some way?

Hello, everyone. Recently, I have been stimulating jurkat cells to secrete IFN- γ, but jurkat cells were stimulated with 0, 1, 2, 2.5, 5 μ g / ml PHA or 0, 1, 2, 5, 10, 20 μ g / ml tuberculosis...

20 September 2022 7,009 0 View

How can I demonstrate that my protein of interest is binding intracellular plasma membrane and then relocating to the membrane at the cell surface?

I have a protein that seems to relocate from the cell surface to an intracellular compartment when phosphorylated. When not phosphorylated the same protein appears to be associated to the cell...

16 September 2022 3,096 1 View

How EPS affects RNA isolation?

Hello, I have problems with RNA isolation from gram positive bacteria producing EPS. I obtain a cloud instead of the classic clear rRNA bands. I tried with liquid nitrogen or beads to break cells...

15 September 2022 4,643 1 View

Is there any tool for risk of bias assessment of case series?

We are doing a systematic review and the majority of the studies meeting our inclusion criteria are case series. We are unable to find any risk of bias assessment tool specifically for case...

12 September 2022 5,667 3 View

Which commercial extrinsic fluorescent dyes can we use to monitor protein unfolding & aggregation?

Hi All, In our lab, we use ThT, ANS, and Bis-ANS dyes to analyze protein characteristics after unfolding. Now, we are planning on obtaining new extrinsic fluorescent dyes. We have a list...

11 September 2022 3,427 0 View

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