I have experience with breast cancer stem cells (CSCs). However, there are no 100% effective ways to cultivate only these cells, as far as I know, as they have the ability to differentiate. I usually phenotype the Cell lines I work with, and some are 100% CSCs by such methods (ALL CD24-/low and CD44+; and/or all ALDH+), and I see how such a population is impacted. Spheroid or organoid formation assays (3D culture types) with some fluorescent tracers is a good way to trace CSCs. Some work by performing isolation by cell sorting (FACS) of both tissue and strains, but this does not guarantee that they will remain undifferentiated throughout the experimentation period.
Felipe Andrés Cordero da Luz Thank you for the insight. It's the differentiation I was worried about. Wonder if that would be an issue in conducting antiproliferative assays.