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I have been trying to transfect primary mouse neural progenitor cells (wildtype and heterozygous for our gene of interest) with a plasmid expressing dCas9-VP64 and a plasmid expressing sgRNA for...
26 February 2025 7,737 1 View
My name is Andre Suryaningprang, I am a lecturer at the Universitas Indonesia Membangun Bandung, West Java, Indonesia Why my campus not listed on affiliation list researchgate? its my campus...
17 February 2025 2,807 1 View
Research About a way to valorise Medium Companies on french market, before a M&A transaction, with just french accounting data.
16 February 2025 4,399 0 View
I completed the sample preparation but have trouble knowing the cysteine residues involved in the disulphide bonds.
06 February 2025 5,765 0 View
I expect a 16 kDa recombinant protein to be expressed in E.coli. However, I could see 45 kDa protein in the SDS PAGE gel after induction. My protein has (His)6 tag, but no bands seen at 16 kDa and...
02 January 2025 2,122 4 View
Which one is easy and most relevant to apply for Post Doc research? 1. Time-Varying Copula Models 2. Diebold-Yilmaz Spillover Index 3. Machine Learning
28 December 2024 262 2 View
Any time I load the pdb file of the complex in the attached file (image), I encounter this error. How do I correct such error? Error parsing HETAT, expected chain ID at column 22, but got...
25 November 2024 5,986 0 View
I am designing primers to amplify gene of approx 6kb length. I am testing gene families which are located adjacent to each other on the genome with high similarity (63-83%). How do I design...
19 November 2024 9,822 2 View
Hi All, I nee script python to detect endpoints information(path, method, parameters) from code source of java farmwork such as Spring boot, jax-rs
19 November 2024 7,103 2 View
I am examining the immunosuppressive activities of several medicinal plants on pristane-induced lupus. The result of the cytokine levels and urine protein are promising, but the histopathological...
13 November 2024 5,596 1 View
I am working on Muti-wall carbon nanotubes and I would like to know if it is possible to do the Rietveld refinement of their XRD data?
11 November 2024 2,635 4 View
Error in user input: Invalid input values In option s Required option was not provided, and the default file 'topol' does not exist or is not accessible. The following extensions...
11 November 2024 6,245 0 View
I read in a bunch of papers where the authors applied genome-wide scans for selection (e.g. population branch statistics (PBS), integrated haplotype score (iHS) etc) that the significance...
22 October 2024 2,894 0 View
Is the best method of measuring gene expression more accurate or measuring protein?
09 October 2024 5,456 4 View
By choosing (the overlap amount) 's=5 pixels', I have patches that overlap by one pixel with three neighbors. I wanted to know if we take this into consideration. How do we choose the value of...
04 October 2024 8,369 0 View
I'm selecting the pipette tips to use in our new lab. We are going to buy bulk tips and use them after autoclaving them. I found that some products are described as "pyrogen-free" and some are...
03 October 2024 168 2 View
I have been performing zymography and am having little difficulty in band interpretation. my gels are not looking properly what can i do to improve that? and the analysis of the gel's band...
28 August 2024 1,718 1 View
Hello, I am looking to identify specific cells with high expression of marker genes using single-cell transcriptomics. Should I find cell populations expressing these marker genes at high levels...
23 August 2024 6,920 1 View
We are creating an Dictionary software.
06 August 2024 8,719 0 View
Greetings How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342?
25 July 2024 6,542 1 View
Hello every researcher I'm performing gelatin zymography to test whether MMPs of the cell would increase or not under the drug treatment, I followed the protocol (attached file you can see the...
22 July 2024 3,576 0 View
What possible theories best explain this situation? An employee was faithfully carrying out his duties and tried his hardest to assist his constituents; however, his colleagues accused him of...
12 July 2024 4,231 0 View
hi I tried working CRISPR activation system to overexpress a gene which is normally not expressed in the target cell. but I failed it. I delivered each plasmids using aav (2wks infect) and already...
04 July 2024 3,141 0 View
define cd
07 June 2024 9,702 5 View