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Questions related to Assays
I am currently performing DNA extraction for a transduced cell with a virus but whenever I perform PCR, there is presence of viral DNA in it. So may I know if adding DNase into the cell before...
14 June 2023 1,993 3 View
If I have a dry ethanolic extract of propolis (after rotary evaporation) How to dissolve it in DMSO ? which concentration of the extract is needed in DMSO in order to be dissolved? And after...
14 June 2023 9,322 0 View
Water samples collected from a lake have been acidified with nitric acid and have turbidity less than 5NTU. Is it necessary to digest again for analysis in ICP-MS?
13 June 2023 5,054 1 View
I've been trying to isolate both mitochondria and nuclei from the rat brains that I work with. But when I run blotting some proteins appears in every fraction of the sample, B-actin and COX4 for...
12 June 2023 4,525 1 View
Once TMB is added the color starts to become blue, but what is the best time to add stop solution? Any method to monitor it or determine it? Why? Thank you !
11 June 2023 8,922 4 View
If there is a modified E. coli strain and we want to observe if it produces more fatty acids than the non-modified, what kind of analysis, tools, equipment are needed? Is there a simple...
09 June 2023 1,913 4 View
Hi there, Could anyone recommend a cheap and easy way to assay for cell death (probably apoptosis). When our gene of interest is transfected into cells, there appears to be a lot of cell death...
07 June 2023 3,301 6 View
Hello everyone, I am working with ELISpot technique and have been using ExtrAvidin® –Alkaline Phosphatase from Sigma-Aldrich (product code E2636). However, this product seems to be experiencing...
06 June 2023 2,633 1 View
Could someone please help me understand how to carry out a kill curve experiment? This is for antibiotics G418 and Puromycin for CHO cells
06 June 2023 2,287 0 View
Why some genes first clone intp p-Twist vector through Gibson asssembly and then clone into p-BAD through Golden gate assembly method?
05 June 2023 4,216 0 View
What could be the possible reasons why my hydroxyapatite (HAP) under ultrasonication-calcination method is bigger in crystalline size than my hydroxyapatite which is calcinated only? The theory...
03 June 2023 4,255 1 View
I would like to be able to go from units of enzyme activity in volume to mass of protein, without knowing the concentration of the protein, I appreciate any help
31 May 2023 4,181 0 View
Hi, I'm going to start to work with quercetin dihydrate as a chemotherapeutic agent. Can I make a stock solution (DMSO) and store it at -20 ºC for several months? I found different information...
30 May 2023 6,117 1 View
By using the extract without the help of a cell line, an animal model how to analyze the neuroprotective activity of the plant extracts. suggest some analysis such as ACHE (Acetylcholinesterase).
25 May 2023 9,181 1 View
Hello! I am preparing a protocol for cell lysis of tissue samples. I want native conformation of as many proteins as possible and would therefore like to have good pH control. In most protocols,...
25 May 2023 3,355 6 View
Microfluidics / Double emulsions
25 May 2023 3,173 0 View
I tried to express a protein in HeK 293T cells cloned in pcDNA 3.1 c myc his, I checked the transfection efficiency by co transfecting gfp vector. It showed good transfection efficiency. But when...
23 May 2023 4,226 4 View
In illumina sequencing results how to rank genes on the basis of false discovery rate (FDR)? What FDR values should be considered as significant?
19 May 2023 7,707 2 View
Hello everyone, Please I am trying to prepare different concentration of doxorubicin for my cancer cells line, Can someone help me with the calculations so I can continue my experiment. Thanks in...
19 May 2023 696 2 View
What are the methodologies involved in sequencing and aligning the genome of an unknown species of insect for the purpose of constructing a phylogenetic tree? Are there any institutions or...
18 May 2023 1,121 2 View
Mokaddis , G. S and Zaki. S. S., The Problem Of The Queuing System M/M/1 With Additional Servers For A Longer Queue. Indian J . Pure Applied Math.1 Vol. 3, No. 3, PP.78-98, 2000. 2. The limit...
16 May 2023 1,796 0 View
If i have to dilute to make working enzyme stock solution how do i do that? kindly help me out
16 May 2023 3,101 0 View
Would anyone be able to recommend me any reference dedicated to determining False Discovery Rate? In the literature (social sciences), it is set at 0.05-0.1; I am mainly interested in learning in...
16 May 2023 2,058 2 View
Actually i used plant extract to determine whether there is antibacterial activity or not.
15 May 2023 5,172 4 View