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Questions related to Assays
i have tried invitrogen pureLink RNA mini kit, and It didn't work
14 December 2023 4,379 3 View
Is there any specific reason not to change strain of the mice for the same cell line?
13 December 2023 5,724 5 View
I’d like to do it in house and figure out which reagent makes the solution so viscous, it’s not good for a fluidic methods. I would like to know if anyone tries anything different to resuspend...
12 December 2023 10,000 0 View
I'm working on my thesis formulating a water-insoluble adhesive to be used for insect sticky traps. what tests should i employ related to this type of adhesive. anything is helpful thank you.
12 December 2023 8,455 1 View
Service Automation: Improve customer service through ticketing, knowledge base, and support analytics.
09 December 2023 8,225 1 View
Can anyone explain why mitochondria are intricately linked with the regulation of inflammation? Why do mitochondria behave differently in different cell types in the context of inflammation? Why...
06 December 2023 2,140 3 View
Hi, can someone suggest some research about the negative correlation between DPPH and FRAP values? Is it possible to determine a decrease in DPPH values while FRAP values increase in the same sample?
05 December 2023 8,582 1 View
A reference that contains the amount of microalgae biomass and the amount of solvents
04 December 2023 10,090 1 View
Ionic liquids synthesis and purification is important task in chemical laboratories because of the liquid phases.
04 December 2023 574 3 View
How to standardize methods for determination of drug residue in tissue by HPLC?
30 November 2023 4,298 1 View
Hello, I am interested in studying the interaction between RNA and proteins, specifically focusing on a particular protein and its corresponding regulatory gene, in mice aortic tissue. Could you...
29 November 2023 4,190 0 View
DD PCR
29 November 2023 2,042 2 View
the fungal tested is fusarium oxysporum and the plant extract i used for treatment is tridax procumbens. i have no idea why there's a lot of white dot appear. is it contamination from bacteria?...
28 November 2023 8,531 4 View
Hello, I was not able to find a natural inhibitor for Fibroblast Activation Protein (FAP) in the banks or in the literature. Even among broad spectrum inhibitors Is there any identified? Thank...
27 November 2023 736 2 View
What techniques are required for identification.
22 November 2023 7,612 3 View
Hi , My over all experimental goal is to define Zika virus-specific T cell responses in macaques infected with Zika virus. We also have control macaques that were not infected with Zika. We used...
17 November 2023 5,678 0 View
Please suggest that DOAJ index medical journals that are low-cost and have a fast-track publication method ?
14 November 2023 8,044 0 View
antimicrobial
12 November 2023 6,373 4 View
Hello every one I Have some problems with tissue wash off when im trying to IHC. I tried some adhesive slides but I didnt get result. my sample is trachea with a lot of cartilages. has some body...
10 November 2023 4,534 2 View
Hi all, I need to do a western blot of a protein where one isoform is ER-membrane-bound. My antibodies do not show that isoform reliably and someone suggested it could be lost due to my RIPA...
09 November 2023 7,139 0 View
How will select the solvent for extracting unknown compounds using soxhlet? Is it possible with TLC? If any kindly provide reference for it?
08 November 2023 3,087 2 View
I'm trying to find the IC50 concentration of 5-FU, oxaliplatin, and cisplatin in HT29 cells. While doing the MTT assay, the drug-treated cells (low conc. ones) showed more absorbance than the...
06 November 2023 6,513 0 View
what is the mechanism that causes antagonism between plant extracts?
04 November 2023 3,062 2 View
I complexed my sample with DNA and obtained an image. However, the image shows some migration above the actual gel height (shown by arrow). Does anyone have an of what could be the reason for this...
03 November 2023 6,360 3 View