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In this method, the first layer containing active bacterial culture agar is spotted. After 48 hours when the bacterial colony has grown, the second layer of soft agar containing indicator bacteria...
24 February 2023 8,893 4 View
Hi, I have list of microbes with me, and I want to check whether particular microbes have probiotic applications. Is there any database for this particular application. At least I can get...
23 February 2023 1,500 1 View
Hi all, I am trying to differentiate SHSY5Y human neuroblastoma cells into neurons with the retinoic acid protocol. I add 10uM retinoic acid into 3% FBS containing DMEM for three days, and...
22 February 2023 8,420 4 View
Every solution I synthesize has a high pH = 8 or above. (I had synthesized my solution by Bohner protocol) What are your recommendations to fix this problem?
18 February 2023 4,043 1 View
I was running a 100ns MD simulation and in the process the system was shut due to power cut. Is there any way to resume the simulation?
17 February 2023 8,157 3 View
I am carrying out diet analysis of some large herbivore via metabarcoding. Now i wish to know whether i can use qiamp fast DNA stool kit for extracting DNA from its fecal/stool samples .Please...
15 February 2023 8,826 0 View
Does somebody has a pharmacokinetics model available in Excel? I’m working with a lot medications and would very much like to know how it builds up and to what extent. A simple model suffices but...
15 February 2023 7,328 4 View
We are attempting to differentiate hiPSCs to cortical neurons and will be growing them on glass coverslips. We have to triple coat the glass with poly-L-ornithine (PLO), laminin and fibronectin....
14 February 2023 8,648 0 View
The bacterial cell wall is primarily made up of peptidoglycans (PG). Thus in order to synthesize the PGs the bacteria require amino acids.
14 February 2023 2,420 3 View
Currently, I wanted to perform an experiment where I have to perform a drug treatment and siRNA transfection together. What shall be the way to design the protocol? Shall I perform the drug...
13 February 2023 6,822 2 View
I am trying perform esterase assay for my fungal isolates directly. I am looking for a complete protocol. Moreover, Is it necessary to pure the enzyme first or we can perform it on our isolates...
12 February 2023 9,597 0 View
I want to measure reactive oxygen species in whole blood. Can anyone please suggest a protocol or method for it using 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) dye ?
10 February 2023 3,576 1 View
We are using a Klenow fragment like enzyme in qPCR assays with Taqman probes. Contrary to expectations, we obtain good amplification curves, despite the enzyme lacks 5'-3' exonuclease activity....
10 February 2023 7,651 0 View
Hello, I'm trying to preform an esterase assay to test PETase enzyme, but the substrate (p-NPA) keeps turning yellow the moment it touches the buffer solution (Sodium phosphate pH 8). From what I...
06 February 2023 9,104 2 View
Hi. I am a student. My analysis is on the difference between male and female treatment outcomes. My t-test analysis showed one-sided p value to be significant (0.046; p=0.05) but my two-sided p...
03 February 2023 7,228 8 View
Hi everyone, I need to make CRISPRi (CRISPR intereference) with a gRNA designed not in the promoter, but in the middle of the gene in an intronic sequence and I am designing the dCas9 plasmid. I...
03 February 2023 901 0 View
In case of potentiometric or any other titration, blank response is not nil. So, what criteria should I set for blank response to demonstrate specificity?
02 February 2023 8,647 2 View
I am measuring the level of progesterone hormone in dogs. There is any difference between measuring the hormone in plasma or serum? And if any, which one is better to use?
01 February 2023 4,889 0 View
I need maximum concentration of chitosan to maintain the positive zeta potential of calcium nanoparticles. Please add your views....
31 January 2023 8,810 4 View
Hi to everyone! I'm carrying out germination tests with onion seeds. Because of a high humidity is important for onion germination, I must place the seeds in Petri dishes which are plenty of...
31 January 2023 7,211 3 View
Dear colleagues, is anyone using SOD assay kit 19160 from Sigma-Aldrich on homogenates (my intention is to measure activity in muscle and brain)? Do you have a validated method how to proceed?...
31 January 2023 8,245 1 View
Hello all, My lab is studying DNA damage in breast cancer cells using alkaline comet assays. About half of the comets I would count as comets are not being recognized by OpenComet. Sometimes it...
30 January 2023 3,016 2 View
I am using the Tecan MagicPrep automated platform to prepare NGS libraries of PCR products from DNA extractions. https://lifesciences.tecan.com/magicprep-ngs?p=tab--7 Despite its fairly recent...
30 January 2023 276 1 View
For example, 1) Rapamycin can augment autophagy by inhibiting PI3K/AKT/mTOR signaling; Uro-A acts as an inhibitor of Akt phosphorylation, thus reinforcing autophagy via the...
28 January 2023 8,616 1 View