What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

Which staining method is best for Archaea?

Generally to differentiate between bacteria we use gram staining technique, broadly based on the composition of their cell wall. However archaebacteria have different cell wall composition, which...

14 December 2020 9,703 5 View

How do I dissolve or disperse ceramic compound Lithium zirconium phosphate in NMP or any other organic solvent?

I want to form composite of pvdf with ceramic compound to dissolve pvdf I am using NMP, how will I dissolve ceramic.

21 August 2018 978 3 View

When not as important to consider solvent effects?

We consider solvent effects to be important for polar molecules, But why are they not important for non-polar/hydrophobic molecules?

14 April 2018 9,931 4 View

When is it necessary to consider size consistency?

I understand that if a given theory for the evaluation of the energy is size consistent, then the energy of the supersystem A+B, separated by a sufficiently large distance so there is essentially...

25 March 2018 5,908 2 View

What is the suitability of PM6, HF, CCSD(T) and B3LYP for these reactions?

To model the first bond dissociation energy of a reaction like: MX6 --> MX5 + X I understand HF is not a suitable level of theory as it cannot describe bond dissociation due to its inability to...

17 March 2018 7,087 4 View

What is the maximum SLS concentration that can be used in enteric coatings?

13 November 2015 1,055 3 View

Any protocol for Biochemical test for fungi and algae?

For the bacterial identification we also go for biochemical profiling, similarly if we are going to identify a novel fungi, can we perform some biochemical test?

01 January 1970 1,126 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View