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Questions related to Assays
Hello, I have an issue with my Alizarin Red staining. It gives random unspecific positivity stains throughout the tissue (Embedded in paraffin, dewaxed in Xylene). My question is, how do I remove...
12 December 2022 9,939 2 View
I am interested in looking at the morphology of the mitochondria in some cells (could be live or fixed). I am not concerned with mitochondrial activity. Is there a simple non-fluorescent stain I...
07 December 2022 3,791 2 View
Hello, I would like to ask your opinions about Alizarin red staining. I am new for the technique. In most of publications the photo of Alizarin red staining shows really red color, however, when...
07 December 2022 5,624 0 View
With standard graph of Sodium nitroprusside how can I determine the antioxidant activity of my pigment in percentage?
05 December 2022 7,656 2 View
two of my T25 flasks, one 6 days old, and the other 3 that have no contamination, with normally colored media and no abnormalities are suddenly floating (~98% of them). 48 hours previous, the...
01 December 2022 1,535 0 View
Hello, I’m currently planning to do a small scale compound screening on a kinase of my internet. I have come across a lot of kinase assays in various formats and I’m confused what assay to...
30 November 2022 3,328 1 View
I need a positive control for a cell cycle analysis experiment. Can anyone please suggest how you would arrest some cells in G0 to be used as a control? Thank you!
23 November 2022 8,057 3 View
I'm just curios if we can make our own cell culture media for the CHO-S cell line. I went through some of the sites for custom make a cell culture media for mammalian cells, and only Cytiva could...
23 November 2022 5,788 3 View
buffer replacement is possible?
22 November 2022 7,367 2 View
Can anyone help me know what I should expect as an acceptable range of endotoxin in EU/ml from my purified recombinant protein sample, purified using E.coli expression vector.
22 November 2022 1,591 4 View
I'm performing a protease assay to plot a standard curve in which BSA is allowed to be degraded by Trypsin, followed by addition of TCA (tri-chloro acetic acid) to stop the reaction, at different...
21 November 2022 3,832 5 View
After a culture period of 7 days with M-CSF, is it needed to still add M-CSF to the cells when you use them in a stimulation assay (for example stimulation with LPS, IFN, TNF)?
21 November 2022 6,105 1 View
I had an experiment about hydrophobic drug release with lipid nanoparticles which have protein (BSA)-polysaccharide shell. I am not sure how much enzyme should I use for protein degradation and...
20 November 2022 6,378 0 View
I need some help with the calculation: Probe lenght: 500bp required 10nM ug/ul=? Considering ave.molecular weight per base= 660g/mol I am either getting very high amount or extremely low, using...
14 November 2022 4,444 2 View
Why is sucrose better than other sugars? I haven't found an articles explaining this question.
13 November 2022 9,483 3 View
I hope everyone is doing well, I'm currently doing a thesis proposal about the cold storage of cucumbers. I already have tentative rating scales but none of the articles I have read has attached...
10 November 2022 828 0 View
I got essential oil using soxhlet extraction with pet benzene solvent. For Anti microbial study , my EO is not soluble with methanol, acetone and I can’t prepare stock solution using DMSO as...
08 November 2022 5,661 2 View
Dear peers, Recently we purchased some NSG mice from JAX lab (#005557) to establish an orthotopic lung tumor model by intratracheal injection. However, we came across some enigmatic issues in...
07 November 2022 1,649 4 View
MCF7 cells grown in DMEM supplemented with 10% FBS, 1% Pen Strep, and 15mM (in the absence of 5% CO2) in T25/T75 Culture flask. Upon seeding in 96 well Plates at the varying concentrations (5000,...
02 November 2022 478 1 View
Hi everyone, I've already thawed my cells (SH-SY5Y) and started to seed them into flasks with using fresh media by adding 1% Amphotericin B that is antifungal, to the culture media. But,...
01 November 2022 2,814 0 View
I'm running qPCR on some microRNA samples and I need a new cDNA kit as the MystiCq one was discontinued. I still have all of the MystiCq primers and ready mix, but need a new cDNA kit, I tried...
01 November 2022 5,309 1 View
I have files with TRINITY id code and differential expressed values as well as the p values after a de novo transcriptome assemply. What is the proccess after this step to identify which are the...
01 November 2022 8,811 1 View
Hello everyone! Hope you are fine and doing well. I would like to know if someone has worked with HMGB1-Gluc construct recently and I would be highly grateful if he or she could forward some...
31 October 2022 4,792 0 View
Hello guys, I am doing a transcriptome analyses, looking specifically at lncRNAs expression. Does it make sense to analyze them according to witch chromosome they belong to? I mean, is there a...
30 October 2022 7,512 1 View