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Hello everyone, Does anyone have suggestions on how to achieve complete or almost complete stripping for the western blot membrane? My protein of interest has a molecular weight of 110kda and my...
20 July 2023 665 3 View
Hi, I has a question regarding infection models- If you are inducing an infection model (bacterial) and then snap freezing the infected tissues in liquid nitrogen afterwards, does the pathogen...
19 July 2023 4,670 1 View
Hey everybody, I recently started analyzing live cells in the Operetta system. They are stained with Tubulin tracker (633) and Mito tracker (546) and no nuclei stain since I want to keep them...
19 July 2023 4,089 1 View
I want to develop negative allosteric inhibitors of CCR2 , which is one of the GPCR , what in-vitro assay is used to know if the tested compound acting as allosteric modulator?
18 July 2023 4,169 1 View
How can I transform the graph from concentration vs. absorbance to concentration vs. velocity in my research on human liver cytosol using spectroscopy to estimate the Km and Vmax values? I have...
17 July 2023 8,279 5 View
I want to perform an antiviral assay for HIV inhibitors. I can't do the p-24 assay and I can only do the beta-galactosidase assay. So which cell lines can I use for this other than the tz-mbl cell?
17 July 2023 6,854 2 View
I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks
14 July 2023 4,983 0 View
We are trying to develop an assay demonstrating the death primary human cells as a result of the membrane attack complex. Our source of complement proteins is normal human serum. We are in need of...
14 July 2023 7,426 2 View
Hi everyone! I am trying to find the DNA sequence upstream of the endogenous COPB1 gene. (ENSG00000129083) However, I am having a hard time finding it, even in the genome viewer. How do I find the...
13 July 2023 6,591 3 View
Anybody know that the dose of tnf-a IP into 3-4week mice .If adult mice is 50ug/kg,then what's the young mice ,according to body weight?
11 July 2023 7,790 1 View
I would like to know the extinction coefficient of EPIRUBCINE for the quantification of the drug. Anyone working on this drug who have used extinction coefficient for the quantification kindly let...
10 July 2023 6,066 0 View
-Various ways to measure PARP enzymatic activity -How to optimize PARP assays
08 July 2023 3,439 0 View
We are performing a pancreatic lipase inhibition assay using para nitro phenyl butyrate as the substrate and Orlistat as the standard in the range of 9-150ug /total mL. Contrary to what we were...
07 July 2023 7,349 2 View
Can we visualize protein produced by topically applied engineered bacteria using 3D skin model? I plan to topically apply the engineered bacteria on the3D skin model and visualize distribution...
06 July 2023 1,673 0 View
Hello, I'm currently having an issue with making an SDS-PAGE gel. After polymerization, big air bubbles form between the gel and glass plate. Although it doesn't appear to have a significant...
05 July 2023 3,187 0 View
Hello everyone! I'm looking for Mnova for Mac, but the license is quite expensive when I checked. Is there any free license available for academics? Alternatively, are there any other comparable...
05 July 2023 2,213 0 View
Generally we tune a model for training. So, is it necessary to tune VGG16 for feature extraction?
04 July 2023 9,339 0 View
We want to buy a new cytometer. Has anybody heard anything negative about MACSQuant 10 or Cytoflex? As we cannot afford maintenance service, it is important for us, that the machine runs smoothly...
30 June 2023 7,559 0 View
I have made a study in the area of mobile advertising and my manuscript has around 15,000 words. I am trying hard to concise the word length but it is affecting the quality of my paper. Please...
28 June 2023 6,239 3 View
Within the scope of my research, I am currently engaged in conducting an estimation of antioxidant power using the FRAP (Ferric Reducing Antioxidant Power) method. To establish a standard curve...
28 June 2023 9,137 1 View
I tried to amplify TOPORS gene of around 3,000 bp from cDNA template with no success. I use Phusion High-Fidelity DNA Polymerase to amplify the TOPORS gene. DMSO was used as well as TOPIRS is...
28 June 2023 1,913 4 View
I am working on HUVEC cells in order to make the Oxidative Stress Injury Model Induced by H2O2.I treated the cells with 0.3% H2O2(30%) for 16 hours, then IC50 was determined by MTT test, but in...
27 June 2023 5,750 7 View
What is also known as marketing memes A marketing technique that can be used in social media marketing and take advantage of the trend advantages
27 June 2023 6,623 6 View
I'm doing some tests with this biomaterial and I came across this challenge when observing cell viability in the fluorencence microscope.
26 June 2023 8,858 0 View