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Questions related to Assays
How to do testing magnesium in iron ore using titration?
02 June 2023 8,658 0 View
Does anyone have experience with culturing CHO DG44 cells? Contrary to the literature, where they should double in 1-2 days, in our case this happens in 1-2 weeks. Has anyone else experienced...
01 June 2023 6,744 0 View
Which is the Best way to make Dry DMF，DMSO,2-ME AND IPA? The water is less 50ppm， Molecular sieves cannot be used。
01 June 2023 2,820 1 View
I would like to be able to go from units of enzyme activity in volume to mass of protein, without knowing the concentration of the protein, I appreciate any help
31 May 2023 4,193 0 View
I would like to be able to go from units of enzyme activity by volume to mass of protein, without knowing the concentration of the protein, I know the solution of the enzyme and the mass of the...
31 May 2023 9,889 2 View
I would like to be able to go from units of enzyme activity by volume to mass of protein, without knowing the concentration of the protein, I know the solution of the enzyme, any help is appreciated
31 May 2023 1,301 0 View
I wonder if someone can introduce me a company selling InGaAsSb for themophotovoltaic research. thank you
31 May 2023 7,953 1 View
Hi, I'm going to start to work with quercetin dihydrate as a chemotherapeutic agent. Can I make a stock solution (DMSO) and store it at -20 ºC for several months? I found different information...
30 May 2023 6,137 1 View
Oxaliplatin (OxPt) has to be dissolved in a solvent to drug CRC cell lines to generate resistant cell lines. The dissolved OxPt would also be used in various other experiments. What is the most...
29 May 2023 4,728 5 View
Greetings, Through my research on food allergen proteins of dietary origin, I have encountered an intriguing case where a particular protein exhibits solely linear epitopes. Surprisingly, no...
28 May 2023 2,887 0 View
27 May 2023 4,667 0 View
By using the extract without the help of a cell line, an animal model how to analyze the neuroprotective activity of the plant extracts. suggest some analysis such as ACHE (Acetylcholinesterase).
25 May 2023 9,198 1 View
Hello! I am preparing a protocol for cell lysis of tissue samples. I want native conformation of as many proteins as possible and would therefore like to have good pH control. In most protocols,...
25 May 2023 3,371 6 View
Microfluidics / Double emulsions
25 May 2023 3,185 0 View
I tried to express a protein in HeK 293T cells cloned in pcDNA 3.1 c myc his, I checked the transfection efficiency by co transfecting gfp vector. It showed good transfection efficiency. But when...
23 May 2023 4,241 4 View
Can aminopeptidase be used with proteinase k in plasma protein digestion? I hope to digest the protein before DNA extration. Anyone know that? Thanks a lot.
23 May 2023 3,843 0 View
Our Lab's FTIR knob broke. Can it give right results without the knob touching the polymer just by the diamond sensor in FTIR?
21 May 2023 7,491 2 View
In illumina sequencing results how to rank genes on the basis of false discovery rate (FDR)? What FDR values should be considered as significant?
19 May 2023 7,725 2 View
Hello everyone, Please I am trying to prepare different concentration of doxorubicin for my cancer cells line, Can someone help me with the calculations so I can continue my experiment. Thanks in...
19 May 2023 713 2 View
What are the methodologies involved in sequencing and aligning the genome of an unknown species of insect for the purpose of constructing a phylogenetic tree? Are there any institutions or...
18 May 2023 1,141 2 View
Mokaddis , G. S and Zaki. S. S., The Problem Of The Queuing System M/M/1 With Additional Servers For A Longer Queue. Indian J . Pure Applied Math.1 Vol. 3, No. 3, PP.78-98, 2000. 2. The limit...
16 May 2023 1,808 0 View
If i have to dilute to make working enzyme stock solution how do i do that? kindly help me out
16 May 2023 3,116 0 View
Would anyone be able to recommend me any reference dedicated to determining False Discovery Rate? In the literature (social sciences), it is set at 0.05-0.1; I am mainly interested in learning in...
16 May 2023 2,080 2 View
I have made a standard curve of p-Nitrophenol concentrations. Now I want to apply the trendline equation on a huge dataset simultaneously to find the unknown concentrations from ODs. Which is the...
16 May 2023 859 0 View