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Questions related to Assays
I am currently performing comet assays and wanted to score them using the ImageJ PlugIn "OpenComet" recommended in other researchgate discussions. To check what the software is actually doing, I...
13 August 2022 3,376 0 View
After incubating whole blood, treating with colecimid, KCl, and fixative, when I drop the mixture onto slides and look at it there is excessive cell debris in the background and few metaphase...
12 August 2022 9,428 0 View
Dear fellows, I am trying to produce and titrate Adenovirus-2 (VR-846 form ATCC) specifically on Vero cells. I had tested several protocols for this but it is not perfectly working. If possible,...
09 August 2022 9,382 2 View
Hi. I am using a BCA protein assay. Using 200uL of Working Reagent and 10uL of the protein isolate. I am reading the results at 562nm absorbance (see image). After that point, should I dive the...
29 July 2022 3,051 1 View
We have compared a multiplexed luminex assay with the standard singleplex assays for ferritin, sTFR and RBP. The luminex values are manifold higher. We have used standards and purified proteins...
16 July 2022 5,239 0 View
Hi! I am measuring changes in glucose uptake in primary adult cardiomyocytes after addition of fatty acids. I am using a 1:100 dilution of CD lipids. Glucose uptake is measured by fluorescent...
15 July 2022 947 1 View
I'm trying to use LPS to induce inflammation in HEK293 cells.I've tried different LPS concentration and incubation time, but qPCR result showed no difference in cytokine expression. Does anyone...
01 July 2022 6,834 2 View
Hi, I'm working on a project where we're using S. aureus and corresponding bacteriophages. I did a spot plate and found out that my phage stock is roughly 2.0x10^7 PFU/ml. I want it to be at least...
27 June 2022 7,912 3 View
N.B: Our LnCap cells died after using 8 hrs starvation. We used 1000 cells/well. How can we get good results in MTT for LnCap cells? Your suggestions will be highly appreciated!
24 June 2022 2,832 2 View
Hi friends, Last week I carried out an experiment to detect the effect of an agent of interest on KCL-induced alpha synuclein aggregations in SH-SY5Y. We used three wells in a 24-well plate as...
16 June 2022 8,721 8 View
Dear colleagues The MUSE cell analyzer has the Muse® Annexin V & Dead Cell Kit for apoptosis analysis . In the kit description, it is informed that the dyes Annexin V and 7-AAD are used for...
14 June 2022 9,520 2 View
I am searching for the main question "why we need to study SPR" 1. Is commercially available SPR devices are not giving enough sensitivity? 2. What is the need of SPR market for improvement? 3....
08 June 2022 1,280 5 View
Hello everyone, I was wondering, since i read a lot of protocols about TIL expansion from solid tumors, what is the specific function of feeder cells for TIL expansion and why are they used in...
05 June 2022 6,614 2 View
Hello everyone, Can anyone show me how to determine or measure the total number of peptide bonds (htot) during enzymatic hydrolysis using the pH-stat method in order to calculate the degree of...
24 May 2022 2,046 1 View
Experimental bacterial growth curves with OD600 in the y-axis typically show rate of growth until a stationary/plateau phase is reached. Theoretical growth curves with the number of cells in the...
23 May 2022 9,618 1 View
Hi, I am a beginner in bioinformatics. I had determine the xyz coordinate of active site for my protein of interest, the coordinate was [69.37, 61.754, 58.738]. But when I repeat the same...
16 May 2022 1,394 3 View
I am currently differentiating PBMC (Monocyte) to iDC using GMCSF and IL4 for 5 days with the change of media on day three by substituting half of the media with fresh media containing cytokines....
26 April 2022 4,179 0 View
Briefly, the question is about the calculation of the molar ratios of Smix i.e. surfactant mixture and the oil phase ranging from 1:9 to 9:1 to get an optimized formulation. 1. the surfactant...
17 April 2022 2,722 1 View
In the literature review, I have found the two strategies to recover folate grafted polymer (PHB or PLGA) in presence of NHS and EDC. 1st rise pH to precipitate out the polymer or secondly add...
13 April 2022 9,294 0 View
Hello everyone, Can anybody suggest to me what would be the best way to use neutral red for phagocytosis in THP-1 macrophages? I have seen many publication papers use neutral red for phagocytosis...
24 March 2022 7,086 0 View
I am looking for cheaper/homegrown options for reliable yields of intact minipig mitochondria. I am interested in the cell phenotype and the mito stress tests, preferably using Seahorse. Also, I...
04 March 2022 7,719 4 View
Also, why there is no blue color formation with the L-serine standard?
03 March 2022 1,926 1 View
I am currently examining the effect of paclitaxel on mda mb 231 and mcf-7 using alamar blue assay. Everytime I analyse the results, I get a straight line of growth inhibition at around 50-60%. I...
03 March 2022 3,771 1 View
HCN is an antimicrobial compound which inhibits the soil-borne pathogen and is produced by Fluroscent pseudomonas. In this case, how plant cyt. oxid. escape from this inhibition, whether any...
28 February 2022 7,814 6 View