Assays

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Questions related to Assays

Does anyone know what the floating pieces are in my cell suspension?

I thawed microvascular endothelial cells from the -80 freezer and spun the suspension in DMEM down at 150G for 5 minutes. After this I added 2 mL EGM-2MV medium to the cells and resuspended it....

23 January 2023 8,390 0 View

What is the significant difference between photocatalysis, electrocatalysis and photoelectrocatalysis ?

Please anyone give us what is the significant difference between photocatalysis, electrocatalysis, and photoelectrocatalysis

18 January 2023 8,790 2 View

How to experimentally validate/prove a membrane transporter protein transports a specific substrate?

To keep this question short, let's say I have a novel membrane protein with the known structural fold of a known transporter superfamily so I know its function is a transporter of cellular...

18 January 2023 7,517 2 View

Are there any cons in using immobilization and running buffers at pH 5.5 in SPR?

Hi, I have always done my in-vitro experiments of protein-antibiotic interaction at a pH of 5.5. Now that I want to do some experiments with the SPR, is it still advisable to use this low pH? Thanks!

17 January 2023 1,301 2 View

PCR product using agarose gel?

I am presently experiencing an issue with my PCR analysis. I ran an agarose gel to see the product size formed from my PCR reaction. Unfortunately, there was no band on the gel, which may imply...

16 January 2023 9,832 5 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

13 January 2023 4,242 2 View

Has anyone detected immunoprecipitated endogenous protein by Coomasie or Silver Stain?

I am trying to detect an endogenous protein of around 135KDa by Coomassie or Silver Stain for a downstream analysis. My immunoprecipitation works well, and the protein seems highly enriched in...

13 January 2023 7,506 4 View

Hello everyone, May I know which immobilization method could be used to immobilize protease?

Should I choose the immobilization method based on the application which I am supposed to do.

11 January 2023 9,106 3 View

Doing alanine scanning mutagenesis faster?

I need to find a mutant allele of my protein that is defective for homo-oligomerization, but can still bind a separate protein (heterodimer formation). I think I will do alanine scanning...

08 January 2023 2,767 5 View

What is the final siRNA concentration to KD a gene in MCF-7 cells? Also share information about transfection reagent for MCF7 cells?

I want to KD BLM and SNAI1 expresson in MCF7 cells. I have tried different final siRNA concentrations (100-500nM) using Lipofectamine-2000 and Jetprime reagent but I am not successful. I have...

06 January 2023 2,536 6 View

How long Incubation time is required when using Rock inhibitor?

Hey everyone, I work with Thiazovivin (TZ, type of Rock inhibitor) on cardiomyocytes cells. Normally, when working with Rock inhibitor (1:1000), incubation of 24h is recommended after passaging....

03 January 2023 394 0 View

How can I prepare a diluent for HRP-conjugated secondary antibody?

I've 1ml anti-mouse IgE conjugated with HRP, I'll use in Elisa testing, how can I make a diluent suitable for use and what is the most perfect dilution ratio to be used?

02 January 2023 3,741 4 View

Analysis of alkyl (ether) phosphates & alkyl (ether) sulfates?

After our published paper about the quantitative purification of alkyl carboxylatic acids via ion exchange, we would like to expand this investigation to alkyl ether phosphates and alkyl ether...

31 December 2022 9,054 0 View

Is there a way to get the distribution, excretion (ADME) compound database?

Is there a way to obtain a compound database containing information of vdss, bbb (blood brain barrier), ppb, Fu (unbound fraction), half life, and clearance corresponding to distribution,...

26 December 2022 4,927 2 View

What will happen if excess of temed is added to SDS PAGE?

Everytime I add the resolving gel, it gets leaked.so is there any remedy to prevent the leakage of the resolving gel?

23 December 2022 7,510 3 View

Can I let macrophages grow directly in transwells or will they already start migrating without a stimulus?

Hi, I am currently working on my master thesis and I want to do a transwell migration assay with bone marrow derived macrophages in January. I have aliquots of the macrophages in liquid nitrogen...

21 December 2022 4,635 1 View

What is the best strategy for biological management of weeds in rice fields?

I would like to know nature-based solutions to biologically control weeds in rice field. It would be great to see a step by step plan of biological control of weeds in rice organic farming.

20 December 2022 5,323 0 View

Any colorimetric phagocytosis assays?

Please suggest any colorimetric based phagocytosis assays for THP1 differentiated macrophages

20 December 2022 6,583 3 View

Why does my DNA oligonucleotide library show multiple bands?

Hello, I am doing whole cell-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process to look for DNA aptamers for the detection of bacteria. I have purchased my DNA...

19 December 2022 2,140 7 View

Sterile filter before drug exposure to cell culture?

Hi! I have to dilute my drug (powder) in 100%DMSO. I need to have atleast 3 serial dilutions in 100% DMSO before the final dilution (0.1% dmso) in the media. the question is should I filter the...

19 December 2022 442 9 View

OT-1 homozygous or heterozygous T cells for T cell killing assay?

I'm new to the cancer immunology field, and trying to develop an antigen dependent T cell : cancer cell killing assay in vitro. I plan to use OT-1/ova. I would isolate T cells from OT-1...

15 December 2022 2,867 2 View

Do photoinitiators/ photosensitizers require lamps with the exact excitation wavelength or can a slightly different wavelength work as well?

Hello all, I am working with tetraphenylporphyrin (TPP) as a photosensitizer for a project. While its excitation wavelength in literature is 405 nm, I only have access to a 365 nm lamp in my lab....

15 December 2022 7,096 2 View

Can SEM-EDS Point Analysis identify elements and its concentration? If so, how?

I want to determine the trace elements (like Na, Zn, Mg, K, Si, Ba, F, etc) of the nanohydroxyapatite that I obtained. Specifically, I want to know if its quantifiable like knowing its concentration.

13 December 2022 9,242 9 View

Immunofluorescence on HT29 cells?

I am working on HT29 cholorectal cancer cells. I have started an immunofluorescence staining protocol on them. I have previously fixed the cells with 2 % formalin diluted from the original 10 %...

12 December 2022 1,751 3 View

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