I am unable to get proper yield of DNA after purification using Qiagen kit is there any suggestions?

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Rahul Tiwari

Not exactly sure what's wrong but please make sure that you are mixing things well and also no amount of sample is touching the pad of the quigen coloum you are using. If all the chemicals are oke and your sample is good then the only problem that can occur is handling. Thank you

Robert Adolf Brinzer

Check the age of the kit. With old kits the zeolite resin in the columns tends to crack.

Emmett M. Smith

What is your starting input? How are you measuring the yield? Is all the ethanol gone after the wash steps?