Assays

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Questions related to Assays

Hello Everyone , Wants to know the latest topic of research in Cyber security??

Wants to know the current topics of research in cyber security

21 September 2025 1,904 4 View

What are some promising open research problems in cybersecurity?

I am planning to conduct a research project in cybersecurity that addresses an impactful and academically valuable problem. My interests include cybersecurity situational awareness, intrusion...

19 September 2025 886 1 View

How should AZD0095 be dissolved?

Hi all, I need to prepare a gavage solution at 10 mg/mL (dose: 100 mg/kg, 200 μL for a 20 g mouse). Could you recommend the best solvent system or dissolution method? Thank you so much in advance!

15 September 2025 1,171 1 View

When designing a high-availability platform, how do you balance scalability with system complexity?

High-Availability Platform must simultaneously meet the following requirements: High Availability: The system maintains continuous operation, with partial component failures not impacting overall...

15 September 2025 1,651 0 View

Can the energy of any electromagnetic photon be calculated with deBroglie's double-particle photon fields equation and the photon wavelength?

https://www.hilarispublisher.com/open-access/on-de-broglies-doubleparticle-photon-hypothesis-2090-0902-1000153.pdf De Broglie’s Double-Particle Photon (Expanded republication PI) Analysis...

12 September 2025 1,617 0 View

How can societies defend against rising autonomous AI-powered cyberattacks?

As AI enables autonomous agents to execute highly targeted and potentially untraceable cyberattacks, cybersecurity firms are scrambling to keep pace. Experts warn of AI-hijacked chatbots launching...

10 September 2025 5,058 0 View

Could simple frequency dephasing be the cause of Zitterbewegung and of the stability of electronic orbitals at some distances from atomic nuclei?

https://www.scirp.org/pdf/JMP_2018042716061246.pdf An Overview of the Hydrogen Atom Fundamental Resonance State... Analysis carried out from the perspective of the Electromagnetic Mechanics...

08 September 2025 5,226 0 View

What volume would you recommend adding to a well of a 12-well plate?

Hi everyone, I am conducting an experiment to induce p-PI3K activation using EGF. However, I am unsure about the appropriate volume of my medium + EGF mixture to add to each well of a 12-well...

20 August 2025 5,403 2 View

Gateway vector for gene overexpression in rice?

Hi everyone, I am new to rice transformation. I was wondering if I could get the names of some Gateway-compatible vectors containing an N-terminal EYFP/GFP tag that are widely used for rice...

11 August 2025 2,961 1 View

How to identify hypoxic areas using pimonidazole in vivo?

Hi all, I plan to inject pimonidazole (MedChemExpress, HY-105129A) intravenously into mice, then collect tissues, fix them in formalin, and prepare paraffin-embedded sections. However, I’m unsure...

11 August 2025 2,528 1 View

MitoSOX Red staining results are not ideal, why?

1. After receiving the dye, store it at -20 ℃. When using it, dissolve 100 μg MitoSOX Red with 26 μL DMSO. Dissolve it at 4 ℃ each time you use it, and prepare it into a working solution at a...

25 July 2025 4,783 1 View

Failed MTT assay on Rat Primary Cortical Neurons following Chemotherapy Exposure? Please help?

Hello! I am just here to ask for some insight about the MTT procedure and why my experiments may have failed. I am completing an honours year and would like any tips or tricks you may have to...

24 July 2025 7,540 5 View

Mystery of using the aging staining kit, why and how?

I used the aging staining kit but the staining was not successful and there were a lot of crystals. What is the reason? My positive control group was not stained successfully either. The x-gal...

17 July 2025 5,144 1 View

Why my citations and h-index differ between Researchgate and google scholar?

https://scholar.google.com/citations?hl=en&view_op=list_works&gmla=AH8HC4yl66YjnWqaUKtBJIwulaQxsImgJxFdH2AMH3c11ZDZgSJWxwSk1t_P3Hn9xlS_FjcyALdvTBAwSnGTX4-hz-Im&user=rlnpHHwAAAAJ

16 July 2025 7,605 1 View

Can Dihydroethidium (Hydroethidine; PD-MY 003) be used for tissue staining?

Could you provide recommendations on whether HY-D0079 Dihydroethidium (Hydroethidine; PD-MY 003) is suitable for tissue staining applications?

27 June 2025 1,872 1 View

WST-8 vs MTT?

Hi everyone, Has anyone experienced ambiguous or conflicting results between WST-8 and MTT assays in cytotoxicity studies? I am currently testing two peptide candidates (SKP85 and SKP179) for...

31 May 2025 8,655 3 View

Please, what could be the reason of HEK293 cells detaching during cell culture???

I would like to understand what can be the reasons why one can be experiencing this.

16 May 2025 5,844 4 View

Citric Buffer Milky/Cloudy from Water Bath?

Hi, I made a citric buffer solution on 3/27 which has been stored in a 5°C fridge. When I transferred this solution to the cuvette today (5/7), and heated it in the water bath (95°C) for about 10...

07 May 2025 7,400 0 View

Why are bubbles forming in my Bis-Tris gel?

This keeps happening to my bis-tris gel during running. Bubbles form in the gel near the cathode wire, and the proteins/dye front stops running at the bubbles. This never happened with the...

23 April 2025 743 3 View

No signal in Western blot: black membrane despite multiple optimizations?

Question: I’m experiencing a persistent issue with my Western blot experiments: I consistently get completely black membranes (no visible bands at all, not even non-specific ones) after developing...

10 April 2025 7,798 4 View

The Arabic version of postpartum bonding Questionnaire?

I am a medical doctor, and I am researching postpartum depression. I need to add the postpartum bonding scale to my research. Could you please provide me with the contact information of the...

05 April 2025 3,251 0 View

Any new research paper in threaded pipe joint?

Objective - Connecting pipes subjected to high internal pressure Material: - fiber reinforced polymers - open to hybrid configurations (FRP and metals)

03 April 2025 8,236 0 View

Can AI systems confirm the validity of a P=NP proof?

Leading AI systems have reportedly confirmed the validity of my P=NP proof and provided a detailed analysis of its theoretical...

12 March 2025 7,359 1 View

What is the best Practical industrial method to reduce Mycotoxin from liquid food and feed ingredients?

I am looking for practical methods to reduce mycotoxins like: DON T-2 & HT-2 Zearalenone Fumonisins Aflatoxin B1 Ochratoxin A

28 February 2025 6,001 1 View

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