Assays

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Questions related to Assays

Can Artificial Intelligence (AI) replace human creativity in some fields and careers? You can give examples?

In this presence age, AI is being used in many areas of human endeavour. Be that as it may, can human creativity, empathy, and complex problem-solving abilities be replaced with AI in any field?

05 June 2024 7,405 10 View

Zombie staining correct gating?

Hello. I am currently working with Zombie NIR and Zombie Violet staining to detect the viability of my neutrophils, but unfortunately I can't see distinguishable populations in either of them so I...

03 June 2024 7,350 0 View

How can I identify an unknown gene of known activity ?

Hi, I've observed a new biological activity in certain cells, likely induced by an enzyme. How can I determine the gene or genes responsible for expressing this unknown enzyme? Thanks in advance

01 June 2024 9,187 3 View

What are the long-term functional and aesthetic outcomes of autologous vs alloplastic materials in facial reconstructive surgery following trauma?

This question aims to explore the comparative effectiveness of using a patient's own tissue (autologous) versus synthetic materials (alloplastic) in facial reconstructive procedures, focusing on...

26 May 2024 1,883 0 View

It is technically correct to meta-analyse antioxidant capacity results from different techniques?

I am doing a meta-analysis, one of the outcomes is about blood antioxidant capacity, but one study has presented its outcome as biological antioxidant potential (BAP), while the others as Total...

16 May 2024 5,260 1 View

How to calculate dosage for cell culture treatment by using plant extract ?

how to calculate dosage for cell culture treatment by using plant extract ?

13 May 2024 7,400 1 View

Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

08 May 2024 4,798 0 View

What are the effects of Atropine on the cardiovascular system?

What are the effects of Atropine on the cardiovascular system?The Role of Atropine in Modern Medicine: Indications, Admini...

05 May 2024 4,070 1 View

What is the main problem of drug development and how to solve the failure conditions of drug development?

WW

23 April 2024 9,677 0 View

What are the recent advances in aromatic plant research?

looking for areas of research

21 April 2024 8,613 1 View

؟Where can I find the details of the foundation of historical buildings and the methods of strengthening that building?

Greetings and regards, I am Samaneh Derakhshan, a senior in restoration of historical monuments. Where can I find the details of the foundation of historical monuments outside of Iran, along with...

18 April 2024 5,271 2 View

What is the definition of the scattering matrix of a unit cell in CST, HFSS and FEKO?

Dear Sirs, I’ve a question related to the Transmission/Reflection coefficients or scattering parameters evaluated by CST, HFSS and FEKO. In particular in Reflectarray as well as Metasurfaces...

12 April 2024 6,498 0 View

In a viability assay of a drug: How to determine if the toxicity of a product is due to its nature or to changes in osmolarity in the culture medium?

How to separate the cytotoxic effect of a molecule from that caused by the change in molarity at high concentrations of that molecule?

08 April 2024 3,819 0 View

Explore and propose a comprehensive ten-year strategy for the advancement of research, marketing management?

can you give me some points or suggestions.

06 April 2024 9,075 1 View

Someone help me to Analyze behvioural test in ischemic stroke mice model especially Cylinder test, pole test and neurological deficit score?

Analyzing behvioural test in ischemic stroke mice model especially Cylinder test, pole test and neurological deficit score is still a big challange for me. Anyone here who will guide me to analyze...

05 April 2024 2,487 0 View

How can I extract and determination PAAs and PAHs in tatoo?

Hi Have a nice day I want to determination PAAs and PAHs in tatoo. Would you present and introduce methods to this?

03 April 2024 6,845 0 View

How to generate divsur.inp for VRC-TST in VaReCoF? I have trouble assigning the Pivot points ..If possible can anyone provide me a syntax?

PivotPoints 1 Frame 0 0 0 0 x1 = 0 y1 = 0 z1 = 0 PivotPoints 1 Frame 0 0 0 0 x2 = 0 y2 = 0 z2 = 0 r1 = r Conditions 0 Cycles 1 r = (5.0, 6.0, 7.0, 8.0, 9.5, 11., 13.,...

23 March 2024 6,315 0 View

What is the storage condition for Bisphenol AF after dissolving with DMSO?

Am having problem with the storage of Bisphenol AF and i couldnt get the proper detailed information regarding the storage can anyone please help me with this issue

13 March 2024 9,309 0 View

How to increase the inter resonator coupling between the two resonators having a common wall between them in filter design?

A slot is made in the common wall between the two resonators. The parameter analysis was done on the slot‘s length , width and its offset from centre to evaluate its effect on the inter resonator...

11 March 2024 5,157 2 View

What is the acceptable level (percentage) of overlap that is reasonable and not affects the acceptance of articles?

Some journals send a similarity-check-reports before accepting any article for publication, this sometimes reach more than 30%. To what extent I need to decrease the similarity and what is the...

09 March 2024 6,796 2 View

How AI has changed the farming and agriculture sector and role of artificial intelligence in agriculture?

How AI has changed the farming and agriculture sector and role of artificial intelligence in agriculture?

07 March 2024 9,367 2 View

I am getting higher seismic demand in terms of displacement for FRP confined RC column as compared to RC column?

I am using concrete01 and the stress-strain parameter is calculated using Lam and Teng model for FRP confined concrete. How to correct the model so that demand for FRP retrofitted will become less.

06 March 2024 7,380 0 View

(SO4)2- anion simulation on Gaussian?

I'm trying to simulate the sulfate anion structure in Gaussian, but my double bonds disappeared after the calculations. I had the same problem when I was trying to simulate the acetate anion....

05 March 2024 557 3 View

What can I use instead of Trolox for the Quenching buffer?

"Hi, I'm conducting research on proximity proteomics using HRP. I intend to assess the functionality of the HRP within the vector I've created by performing biotinylation with biotin-phenol and...

05 March 2024 5,617 0 View

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