Contact experts in Assays to get answers
942 views 2,569 posts
Questions related to Assays
Hi! I am performing PCR quantification of 5 inflammatory markers on 180 samples. As you can imagine, I therefore work on several 384-well plates. To compare them, I introduced a duplicate...
12 May 2023 1,569 9 View
Even though we are mixing the ethidium bromide properly before agarose gel casting We are facing this problem of uneven staining.Its not that the staining is random, but exactly half.We tried...
12 May 2023 373 3 View
I am wanting to measure the cytokines IL-1b, IL-1ra, IL-2 and IL-17 using the DuoSet ELISA Kits with the ancillary reagent kits from R&D. The protocol suggests adding 10-50% of animal serum...
08 May 2023 992 0 View
I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...
04 May 2023 9,021 3 View
Hello everyone, I'm now working on ELISA. I'm testing TNFa on the serum of pigs. The results are pretty inconsistent. I did the 1st plate and everything came out well. But I'm now running a second...
03 May 2023 4,076 4 View
Hi, I have identified an antiviral compound that showed antiviral activity against feline coronavirus. Now, I am trying to figure out its antiviral mechanisms. First, I try to figure out which...
02 May 2023 8,473 1 View
I am preparing myself for western blot of my samples, but I have confusion in preparing the protein samples because there are a lot of protocols given and I am using RIPA buffer specifically for...
26 April 2023 6,000 2 View
What are the techniques used to detect, identify, localize the faults in microgrid/smart grid/ active distribution grid using Phasor measurement unit? Please explain about the techniques about...
26 April 2023 8,938 3 View
I am doing enumeration of bacteriophages with the double agar overlay assay for the phage suspension that was already purified three times. The plaques i get are not all the same size. Does this...
26 April 2023 8,968 3 View
I am currently involved in research for controlling weevils in stored wheat using different essential oils. What could be the best concentration (microlitres per litre of air) of essential oils...
26 April 2023 3,944 4 View
"Restrict from EMBOSS Suit version 6.3.1 with the following parameters: snucleotide1, sitelen = 4, rformat = table, enzymes = enzymes.txt. From the 4379 enzymes present in REBASE, we selected the...
24 April 2023 6,772 4 View
I'm doing qPCR for both DLD1 and RKO cells (cancer cell lines) and amplified two genes of interest for both cell types. 1. Expected results were obtained for DLD1 cells but for RKO cells, qPCR...
20 April 2023 8,790 3 View
I am planning to work with gilvocarcin. I know that it is an antibiotic that has got anti-tumourous effect, as well. As far as I know UV illumination is needed to have the anti-cancerous effect. I...
17 April 2023 3,261 1 View
I have been using the serial dilution method of analyzing the efficiency of Primers designed, via Standard curve. For the assay I take mixture of variety of cDNA's but every time the efficiency...
15 April 2023 4,686 7 View
I want to extract proteins from serum in order to use them in Western Blot analysis but I can't find any protocol for protein extraction from serum. In fact, I would also like to know whether it...
14 April 2023 7,106 3 View
What does it mean that it could have precipitated? What does it mean that NADH solution can be unstable? In some instances the absorbance increased from before to after I mixed in the...
13 April 2023 9,966 0 View
Hello, I am currently trying to do cryo-sections of Xenopus laevis oocytes in order to visualize the cell membrane, but I'm having trouble to get good sections. That's what I tried so far (from...
11 April 2023 7,860 0 View
I'm interested in transforming a Staphylococcus aureus strain with a GFP-expressing plasmid. Is it possible to do it with the Calcium Chloride technique?
02 April 2023 4,411 0 View
I am doing cAMP assay, using three ligands to study their effect on GPCR. I need to prepare 0.5 mM IBMX in stimulation buffer, and mixing it with cell suspention. Is there any specific method yo...
02 April 2023 4,245 2 View
How to dissolve plant ethanolic extract in sterile aquades if it is not dissolving completely? Should i dissolve it first using DMSO or ethanol in low conc before dilute it with aquades?
30 March 2023 2,735 1 View
Please see the attached image for the reference. I have been seeing these cells in the Arabidopsis thaliana seed (post germination) whenever I go for microscopy. The cells are attached to seed...
28 March 2023 3,084 4 View
I treat mammalian cells with drugs and look for target proteins on cell surface (known target in this case). I am doing pull down experiments on mammalian cells to capture the target after lysing...
24 March 2023 2,871 0 View
I am conducting an LCA for stone and would like to know if I can use OpenLCA (the free version) with an existing database that comes with the software? I am open to suggestions.
23 March 2023 9,784 1 View
I was told that it's best to develop my GC detection and quantification method with the single quad mass spec and then switch the method to FID to generate the calibration curves and do...
23 March 2023 9,612 4 View