Contact experts in Assays to get answers
952 views 2,569 posts
Questions related to Assays
Any one has used ELISA based assay to determine the affinity of an antibody? I have a few questions regarding the assay and the raw data, if any one may be able to assist, please.
20 February 2022 3,482 3 View
Hi all, I'm currently doing co-culture of MSCs and PBMCs in a 96 well plate. MSCs are left to adhere for 24 hours, before adding CD3/CD28 activated PBMCs (1:10 ratio). However, I find my...
18 February 2022 5,173 3 View
The antigen that we are trying to detect in pathogens using monoclonal antibody or polyclonal antibody studies, are these not detected when they the organism to be studied is dead? In addition...
16 February 2022 8,999 1 View
Hello everybody, I wanted to isolate phages against Micrococcus luteus from human nose swabs. I tested nose and mouth swabs in a spot assay on M. luteus for phages and the results were clear...
16 February 2022 9,297 2 View
Hello all, I would like to know the toxins which block cytokinesis without blocking nuclear fragmentation apart from Cytochalasin B.
16 February 2022 8,348 3 View
Hello, I have been trying to use the amplex red glucose assay kit (A22189) to measure the glucose in adipose tissue, but when performing it, the standards and all the samples don't show change in...
15 February 2022 8,591 3 View
I am doing research on WNT Pathway. I have multiple drugs and want to do a assay to find out my drugs are WNT activators or inhibitors. I know about TCF/LEF reporter assay kit. But I do not have...
08 February 2022 1,299 4 View
I am working on emulating a blood brain barrier on a chip and to evaluate paracellular permeability we are using FITC-Dextrans. I am wondering if the permeability of intact blood brain barrier and...
08 February 2022 7,832 1 View
Hello Everyone, Greetings! Hope everyone is fine. CBMN assay involves Cytochalasin B which blocks cytokinesis by inhibiting actin filament formation which leads to micronuclei formation in the...
07 February 2022 4,382 1 View
I have a solvent mixture containing methanol/water and methanol/toluene of unknown volumes (each solvent). I need to estimate the methanol content through a quantitative analysis (Qualitative...
05 February 2022 2,443 4 View
via HTS I found Inhibitors (IC50 between 1 to 5uM) for a protein that is involved in a specific pathway. Now I am doing cell-based assays to see the In-vivo effect. I, Incubate the cpds with the...
01 February 2022 8,826 3 View
Hello, can someone help me or guide me through BCA assay, what formula is used in the end to calculate the protein concentration.
29 January 2022 6,616 4 View
I am working with bovine monocytes to assay innate immune response against to S. aureus
27 January 2022 906 1 View
I am currently working on developing a fast/cheap assay of a certain protein in the blood that happens to be an excellent biomarker for a variety of diseases. I plan to be able to run my assay...
24 January 2022 7,419 4 View
I intend to run a simple proliferation assay with PBMC to evaluate whether my PMA reagent is still viable. But I was hoping to at least have a paper showing some reference values regarding the...
24 January 2022 3,820 2 View
I am a student from a relatively developing country and the commercial enzyme is expensive for us. My graduation thesis is desperately in need of an available standard curve for my AchE assay so...
20 January 2022 2,234 4 View
I am going to use chick embryo for angiogenesis development assay. if i want to do a quantification, is there any free software that can be used? or can someone explain how to use ImageJ for it?
18 January 2022 6,208 20 View
I am trying to find out how much cells are viable after my incubation. I am having issues with working out the standard curve. Seed the cells in 96 well plate 'v' bottom and transfer to a flat...
18 January 2022 8,058 7 View
In many studies and researches, the relationship between the water content (or degree of saturation) and Suction were found by different methods: the question is: Is any relationship between the...
17 January 2022 9,159 8 View
how to make the disc of a particular nanoparticle concentration for antibacterial disc diffusion assay
16 January 2022 6,258 4 View
1) Using a secondary culture for inoculation in a minimal medium. 2) Washing the overnight culture cells and then using them for inoculation in a minimal medium. 3) Using directly the overnight...
13 January 2022 7,398 3 View
What is the difference between Fe2O3 and Fe3O4 nanoparticles in creating magnetic properties in MWNTs and SWNTs, PACs, TiO2, and ZnO? What Nanoparticle (Fe2O3 OR Fe3O4)has the strongest magnetic...
06 January 2022 3,009 2 View
hello everyone, i am testing my adsorbent using atomic absorption, an initial cadimium concentration of 100ppm has been tested, the concentration after the assay with atomic absorption is 2ppm,...
05 January 2022 6,625 3 View
I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you,
02 January 2022 7,855 3 View