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Questions related to Assays
Actually i used plant extract to determine whether there is antibacterial activity or not.
15 May 2023 5,194 4 View
With the increase of office hours, the human eye will produce visual fatigue, but with the increase of time and gradually deepen the visual fatigue, how is the change process？Working on different...
15 May 2023 9,699 1 View
Hi! I am performing PCR quantification of 5 inflammatory markers on 180 samples. As you can imagine, I therefore work on several 384-well plates. To compare them, I introduced a duplicate...
12 May 2023 1,588 9 View
Even though we are mixing the ethidium bromide properly before agarose gel casting We are facing this problem of uneven staining.Its not that the staining is random, but exactly half.We tried...
12 May 2023 397 3 View
Please Suggest me complete method for these tests.
12 May 2023 708 0 View
What are the factors affecting the growth and development of plants and environmental factors that affect a plant's growth rate?
11 May 2023 5,078 2 View
I am wanting to measure the cytokines IL-1b, IL-1ra, IL-2 and IL-17 using the DuoSet ELISA Kits with the ancillary reagent kits from R&D. The protocol suggests adding 10-50% of animal serum...
08 May 2023 1,008 0 View
I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...
04 May 2023 9,048 3 View
Hello everyone, I'm now working on ELISA. I'm testing TNFa on the serum of pigs. The results are pretty inconsistent. I did the 1st plate and everything came out well. But I'm now running a second...
03 May 2023 4,100 4 View
Hi, I have identified an antiviral compound that showed antiviral activity against feline coronavirus. Now, I am trying to figure out its antiviral mechanisms. First, I try to figure out which...
02 May 2023 8,488 1 View
Can anyone tell me the price of G-walk BTS Bioengineering?
02 May 2023 3,700 0 View
Are all probiotics can make fermentation?
02 May 2023 8,023 3 View
Hello, I am developing a method for some derivatized metabolites using 3-NPH (nitrophenylhydrazine). I did the compound optimization for the derivatized compounds individually, but when I started...
02 May 2023 6,684 0 View
I am preparing myself for western blot of my samples, but I have confusion in preparing the protein samples because there are a lot of protocols given and I am using RIPA buffer specifically for...
26 April 2023 6,027 2 View
What are the techniques used to detect, identify, localize the faults in microgrid/smart grid/ active distribution grid using Phasor measurement unit? Please explain about the techniques about...
26 April 2023 8,975 3 View
I am doing enumeration of bacteriophages with the double agar overlay assay for the phage suspension that was already purified three times. The plaques i get are not all the same size. Does this...
26 April 2023 8,989 3 View
I am currently involved in research for controlling weevils in stored wheat using different essential oils. What could be the best concentration (microlitres per litre of air) of essential oils...
26 April 2023 3,975 4 View
"Restrict from EMBOSS Suit version 6.3.1 with the following parameters: snucleotide1, sitelen = 4, rformat = table, enzymes = enzymes.txt. From the 4379 enzymes present in REBASE, we selected the...
24 April 2023 6,799 4 View
I'm doing qPCR for both DLD1 and RKO cells (cancer cell lines) and amplified two genes of interest for both cell types. 1. Expected results were obtained for DLD1 cells but for RKO cells, qPCR...
20 April 2023 8,815 3 View
Azocasein was used as substrate and hydrolyzed under protease. The location of characteristic peaks was different under acidic and alkaline conditions. It was wondered under which conditions...
20 April 2023 6,424 2 View
I am planning to work with gilvocarcin. I know that it is an antibiotic that has got anti-tumourous effect, as well. As far as I know UV illumination is needed to have the anti-cancerous effect. I...
17 April 2023 3,285 1 View
I have been using the serial dilution method of analyzing the efficiency of Primers designed, via Standard curve. For the assay I take mixture of variety of cDNA's but every time the efficiency...
15 April 2023 4,711 7 View
Hello. I have the Rotor Gene 3000 amplifier, but I cannot find a program for it. QIAGEN has stopped supporting this cycler and cannot send me the appropriate program. Is there anyone who can share...
15 April 2023 7,651 0 View
I want to extract proteins from serum in order to use them in Western Blot analysis but I can't find any protocol for protein extraction from serum. In fact, I would also like to know whether it...
14 April 2023 7,123 3 View