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I tried to amplify TOPORS gene of around 3,000 bp from cDNA template with no success. I use Phusion High-Fidelity DNA Polymerase to amplify the TOPORS gene. DMSO was used as well as TOPIRS is...
28 June 2023 1,951 4 View
I am working on HUVEC cells in order to make the Oxidative Stress Injury Model Induced by H2O2.I treated the cells with 0.3% H2O2(30%) for 16 hours, then IC50 was determined by MTT test, but in...
27 June 2023 5,782 7 View
What is also known as marketing memes A marketing technique that can be used in social media marketing and take advantage of the trend advantages
27 June 2023 6,672 6 View
I'm doing some tests with this biomaterial and I came across this challenge when observing cell viability in the fluorencence microscope.
26 June 2023 8,907 0 View
I'm studying a system with one binding site that accommodates two ligands simultaneously using isothermal titration calorimetry (ITC). I'm seeking advice on the appropriate modeling approach for...
21 June 2023 563 3 View
What are the internal and external factors affecting plant growth in plants and animals and environmental factors that influence growth and development?
21 June 2023 4,776 2 View
I am losing beads in almost every well of 96 wells plate and also my beads per second is very low like highest was only 5. What could be the possible cause. I take appropriate time between the...
18 June 2023 1,217 2 View
What is acceleration due to gravity show its value changes with altitude and depth and does acceleration due to gravity on earth depend upon?
18 June 2023 1,822 2 View
I extracted RNA from Uterine fibroid tissue by using TRIzol Method.
16 June 2023 3,326 2 View
She is Chilean
16 June 2023 1,296 1 View
Hello everyone. I need help. I am new to cell culture (2 months +) so I am not really experienced to distinguish these cells if they're healthy or not. Is this HEK293 cells contaminated?...
15 June 2023 6,577 6 View
I am currently performing DNA extraction for a transduced cell with a virus but whenever I perform PCR, there is presence of viral DNA in it. So may I know if adding DNase into the cell before...
14 June 2023 2,056 3 View
If I have a dry ethanolic extract of propolis (after rotary evaporation) How to dissolve it in DMSO ? which concentration of the extract is needed in DMSO in order to be dissolved? And after...
14 June 2023 9,386 0 View
Water samples collected from a lake have been acidified with nitric acid and have turbidity less than 5NTU. Is it necessary to digest again for analysis in ICP-MS?
13 June 2023 5,109 1 View
I've been trying to isolate both mitochondria and nuclei from the rat brains that I work with. But when I run blotting some proteins appears in every fraction of the sample, B-actin and COX4 for...
12 June 2023 4,609 1 View
I want to test a list of peroxidases against a specific metabolite. Ideally, I want to find an enzyme that will catalyze the reaction. But even a feeble peroxidase catalytic activity would be a...
12 June 2023 2,590 1 View
Once TMB is added the color starts to become blue, but what is the best time to add stop solution? Any method to monitor it or determine it? Why? Thank you !
11 June 2023 8,997 4 View
If there is a modified E. coli strain and we want to observe if it produces more fatty acids than the non-modified, what kind of analysis, tools, equipment are needed? Is there a simple...
09 June 2023 2,045 4 View
Hello, I am working on the extraction from Total Protein Soluble and then purification of protein expressed in Nicotiana benthamiana leaves agroinfiltrated with Agrobacterium. Could you suggest me...
09 June 2023 7,589 0 View
I was using para nitrophenol acetate for an esterase test in a microtiter plate. The reaction contained just crude enzyme, p-NPA, and also Tris-HCl buffer (pH8). The final product was p-NP(para...
09 June 2023 7,244 0 View
Hi there, Could anyone recommend a cheap and easy way to assay for cell death (probably apoptosis). When our gene of interest is transfected into cells, there appears to be a lot of cell death...
07 June 2023 3,407 6 View
Standard graph of NO and triplicate readings of different sample concentrations with Griess reagent are available.
07 June 2023 6,062 0 View
I tried different concentration of Sudan black B (3 and 0.3%) in 70% of ethanol. I face the same issue when I stained my bacteria, the slide not clear and there are many of sudan black paticles in...
07 June 2023 7,159 0 View
Hello everyone, I am working with ELISpot technique and have been using ExtrAvidin® –Alkaline Phosphatase from Sigma-Aldrich (product code E2636). However, this product seems to be experiencing...
06 June 2023 2,689 1 View