Is it possible to grow a single dish of adherent mammalian cells (e.g. HEK293) where the cells form a continuous layer but there are different media compartments?
We are looking at cell:cell communication so need the cell monolayer to be continuous and permit cells to grow touching each other. But we want some cells to be exposed to media A and some exposed to media B with no media mixing. It doesn't really matter on the configuration of the different media - the interface between the two media can be half and half down the middle, can be in a grid, can be a line bisecting the culture dish etc. Preferably this would work on glass for microscopy, but plastic culture dishes could work if needed. The cells would be maintained under normal mammalian cell culture conditions with general cell culture lab equipment available.
I'm trying to work out how to feasibly set up the experiment but this is new to our lab so I'm not even sure what search terms I should be looking for.
I have not done this, but I would try laying down strips of hydrogel. This would allow eventual diffusion across the wall of gel, but dye studies would tell you if the time was long enough. you could put medium with phenol red on one side and without on the other. https://www.upmbiomedicals.com/applications/for-life-science/transfer-animal-free/?gad=1&gclid=EAIaIQobChMIieb0mqbMgAMVjgizAB0SGwywEAAYAyAAEgLP0_D_BwE
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