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Questions related to Assays
We would like to establish multiplex PCR followed by Reverse Line Blot Hybridization (RLB) technique for detection of different pathogens in our laboratory. Please suggest which equipment that is...
22 March 2023 1,000 0 View
Say example, we isolate the protease producing microbes using casein as substrate in agar. Now we want to test isolated microbes on different type of protein which is also insoluble in water for...
21 March 2023 5,841 0 View
Dear all I want to set up the technique to assess the neuraminidase antiviral resistance in Influenza viruses, using the MUNANA assay. I already have a TECAN fluorimeter fluorimeter Infinite...
20 March 2023 6,361 4 View
Does the benzoic acid used as antifungal agents on to MRS agar?
19 March 2023 3,140 1 View
Hi everyone, please bear with me, because I am a complete beginner with regard to any form of bioinformatics and I am trying to understand the best approach to my experiment. I am currently...
15 March 2023 6,050 1 View
What are the Scale dependent & non dependent parameters of RMG & FBD ? How we should evaluate during Scale up ?
15 March 2023 4,055 6 View
I have been growing SH-SY5Y cell line in DMEM/F12 media supplemented with 15% heat-inactivated FBS. For differentiation, I plated 10000 cells per well in 96 well plates and changed media every...
14 March 2023 7,977 8 View
Hi, I would like to ask on what to do when an ELISA kit protocol requires higher volumes of serum compared to what you have. In my case I have serum volumes of 10 microliters while the experiment...
13 March 2023 3,921 3 View
Hello, I would like to ask you a question. Recently, I injected 100ug OVA subcutaneously into C57BL/6 mice and conducted enhanced immunization on the seventh day. On the 21st day, cells from the...
12 March 2023 7,787 0 View
I have an SPR setup consist of an LED, a lens, a polariser, a prism, an SPR chip and a paper screen. When I tried to see the SPR dip in the air I find it easily. It is around 43-45 degree. But...
12 March 2023 5,752 8 View
Hello, I'm trying to obtain Pharmacophore modelling ligand based of approximately 70 molecules in 2 files, I'm trying to use PharMapper but it always gives error when uploading file (even though...
10 March 2023 3,617 2 View
I have been attempting to purify a streptagII-tagged protein from mammalian cells using streptavidin-magnetic beads without success. The reason I'm using streptavidin and not streptactin is that...
09 March 2023 360 0 View
I have been working on generating a stable CRISPRi cell line in Fibroblast and Smooth muscle cells. The problem is the cells stop proliferating after several rounds of proliferation and loose...
07 March 2023 1,447 0 View
HI! In the lab we're doing a project where we start from a DNA sequence. When this is re-transscribed it would give us the sequency of a shRNA but I would like to know what step/methodology I...
06 March 2023 816 1 View
Dear colleagues, Can you tell me what is the best chemotherapy method for BALB/c mice with colorectal cancer? Thanks in advance
03 March 2023 7,094 2 View
Dear all, We would like to perform a protein extraction from human serum. It will be used for MALDI-TOF analysis (detection of virus). Do you have a suggestion for protocol? Best regards,
02 March 2023 2,831 2 View
I'm planning to process mouse inguinal and popliteal lymph nodes for Luminex assay. Does anyone have experience with it? What reagents to use? Right volume and dilution of the sample etc?
02 March 2023 4,654 0 View
Oral tumor derived primary cells fail to adhere onto the dish. They grow as suspension cells and die after 2-3 passages. It does not look like contamination is the issue. Reference picture is...
02 March 2023 3,037 4 View
I need to prepare a dye to mix with my RNA sample before running on gel. The protocols obtained online contain xylene cyanol as a constituent. Is there a recipe without xylene cyanol? Please...
27 February 2023 8,771 0 View
To obtain pure microalgae cultures I have inoculated the water sample on BG-11 and BBM media using spread plate method. I am getting yellow colored colonies on both the media plates. Kindly...
27 February 2023 7,024 4 View
In a substrate in horticulture, how will the substrate pH affect the availability of Iron? If pH goes down in the substrate, will Iron be reduced from Iron III to Iron II?
27 February 2023 4,744 0 View
To understand my Bioprocessing module and better, I have been reading a lot about bacterial fermentation. I came across a lot of literature that either stuck to heat induction or biochemical...
26 February 2023 4,366 4 View
While performing sonication i am facing the issue of frothing which i think results in the aggregation of desired protein and leads to the formation of inclusion bodies
26 February 2023 816 2 View
Hello all, Our lab used CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit from Invitrogen couple years back. We are now looking to see if there are any up-to-date kits for caspase 3/7 or...
25 February 2023 4,100 1 View