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Questions related to Assays
I will incubate well plate for E.coli biofilm formation. After this I need to perform centrifugal filtration with Biofil 30kDa filters. Are there any tips, how to do it properly? And can I use...
02 March 2024 6,883 1 View
I want to culture Huvecs cells under hypoxic condition (hypoxic chamber), Does anyone know the cell culture and treatment process?
16 February 2024 6,848 0 View
Hi, My research focuses on analyzing and modeling cell migration through the lens of cell shape, specifically by tracking the movement of protrusions like lamellipodia and other cellular...
15 February 2024 7,199 2 View
Hi I'm new to cosmetics analysis and looking for cheats for these products. I need information today about illegal preservatives used in cosmetic shampoos and creams. I know that preservatives...
13 February 2024 9,244 2 View
I performed RNA isolation using NucleoZOL reagent from rat brain tissue (it is very small piece appox. 2-3mg of tissue). As a control, I also did all the NucleoZOL procedure without a tissue in a...
11 February 2024 1,409 5 View
As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!
31 January 2024 3,616 2 View
We've been using the AdvanceBio SEC column 120A 1.9um 2.1x150mm PEEK with an Agilent LC QTOF but recently we have been problems with this column and we were not able to perform any runs.
25 January 2024 9,734 3 View
What are the interactions between microplastics and energy?
17 January 2024 2,156 1 View
Hi all, I have been working on invasion assay but i have encountered a problem recently. I have attached image below (where one of them showed clear morphology while another are dispersed? Your...
11 January 2024 7,107 0 View
Can any one please help me? I have to make a stock solution as well as working solution. For my assay, working solution of 0.01U/ml is needed. I've attached the specifications of enzyme which are...
11 January 2024 512 1 View
what is 1335 in acetyl value and why this value put here as constant? what is 1335 in acetyl value and why this value put here as constant?
11 January 2024 8,461 0 View
I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...
11 January 2024 656 1 View
I want to do a numerical simulation for the polishing and finishing of flat material. please suggest the name of the software
09 January 2024 7,428 2 View
T O N T O A free object-based system for computational chemistry Version: 21.03.16 v. cb74494 Platform: Windows Build-date: 2021-03-16 05:01 For conditions of use, on-line documentation,...
09 January 2024 4,586 0 View
I have downloaded the instruction manual for digital Coffalyser software, installed the software and demo data set. Yet, I am facing stumbling blocks to do the data analysis from the demo set,...
04 January 2024 3,919 0 View
Explore the impact of WebSocket on web app communication, emphasizing real-time updates. Seek insights into its advantages, implementation, and potential challenges from the ResearchGate community.
03 January 2024 2,298 1 View
We are attempting to establish primary cell lines from patient tumor samples of head and neck squamous cell carcinoma (HNSCC). Following transport to LUMS within 30-45 minutes of surgery, the...
02 January 2024 9,200 0 View
I want the procedure for Nitric oxide assay in plant sample.. Please kindly help in this regard at the earliest .. send an email to [email protected].
31 December 2023 9,905 1 View
Does mechanical stress cause microplastic release from plastic bottles?
29 December 2023 7,937 2 View
Can activity enzyme PPO (polyphenol oxidase) carried out using 4-methyl catechol in phosphate buffers pH 7,0? What wavelength can we use to see enzyme activity? Can it read at 410nm or 400nm?...
26 December 2023 1,544 1 View
Hi everyone I synthesized carbon dot with folic acid and citric acid with DMF solvent. After analyzing, it was found that there is no problem with the synthesis. But using a fluorescent...
24 December 2023 4,227 3 View
We know that complete oxidation of one glucose molecule give 38 ATP during aerobic respiration but i found different answers from different sources.
23 December 2023 5,298 3 View
Dear Reader Is there anyone out there who knows or has a cost-effective alternative to the evaporation of excess water from an ammonium sulphate solution? The requirement is to control the...
20 December 2023 2,722 1 View
The catalytic combustion of 100ml methanol VOCs was occurred how we can calculate its calorific value? The vapor pressure is 13kpa at 20Cand heat of combustion is 725kj/mol at 25C.
20 December 2023 7,779 0 View