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Questions related to Assays
Hi, I work with primary culture cells from the periodontal ligament and I'm having difficulty getting them to stick to the bottom of the well so they can propagate. What can I do to make them more...
29 October 2023 8,208 1 View
If both the products are CO2 and H2O, how can we determine whether the process is oxidation or combustion?
25 October 2023 9,802 0 View
Which mobile phase, a wavelenght and a flow rate would be the best? The column is packed with ODP stationary phase. My sample also contains NaOH, H2O2, therefore I need to avoid interference of...
23 October 2023 3,963 2 View
When i make a stock solution of d5- kynurenic aicid (1 mg/ml), the solution is turbid and not clear. Inspite of sonication in water bath with mild heating, the suspension is not clear. Can i...
19 October 2023 9,812 0 View
I'm getting an error on a Freedom Evo. It says Evo_Evo_012_004. Device not implemented. How can I fix this?
18 October 2023 8,451 0 View
I've been trying to prepare a cell culture media (1% BSA) with palmitic acid (PA), but it keeps precipitating. I successfully made a stock by dissolving 1 g PA in DMSO, so that's not a problem...
18 October 2023 8,461 3 View
Recommend the research paper with release pattern studies
16 October 2023 2,664 1 View
My group and I worked on a thesis on spatiotemporal patterns of COVID-19 in the Philippines using ArcGis 10.7. We had no prior knowledge of disease mapping although we were able to come up with...
15 October 2023 641 2 View
hi i'm gonna use crispra system for my study, how can i validate sgRNA not having off-target effect?
14 October 2023 6,756 0 View
I need the information for my upcoming review articles.
13 October 2023 9,252 1 View
Hello everyone, please I have been having difficulties in transfecting DB and Karpas-422 suspension cells with the lentivirus, can anyone suggest or advise me on the possible solution, I have...
13 October 2023 7,681 0 View
Could someone tell me the freezing medium you use to freeze the RT4 and T24 bladder cells? I found on Elabscience (www.elabscience.com) that for T4 it is: 60% basal medium+30% FBS+10% DMSO. For...
11 October 2023 9,504 0 View
Hello, does anyone know the maximum passage number recommended for T24 and RT4 bladder cancer cell lines? Thanks
11 October 2023 7,260 1 View
We have injected GFP and td-tomato viral tracing injection in the mice brains to see axonal projections, and we are using tertiary butyl alcohol instead of ethanol for the dehydration process as...
10 October 2023 8,676 8 View
ASCE or FEMA or Euro Code or IS?
10 October 2023 5,350 10 View
How can the criminal justice system reconcile the need for public safety with that of offender reform and reintegration into the society?
05 October 2023 786 0 View
I seeded RAW cells (passage 12) in a 12-well plate at a density of 5x10^5 cells/well. After 24 hours, the cells appeared shrunken, and there was less color at the edges of the wells. I used DMEM...
03 October 2023 5,752 0 View
APA 7th referencing style in details
03 October 2023 9,633 1 View
I know that thermodynamically less stable premRNA molecules splice more efficiently but the consequences of this remains unclear for me.
29 September 2023 6,154 0 View
How to remove error in dstart and clamddsub
28 September 2023 9,363 0 View
can anyone help me out in the process for removing water from 6% ethanol, 4% lignin and 90% water mixture and retain ethanol and lignin alone.
28 September 2023 6,557 0 View
Hi all, a colleague in our lab has recently characterized 5 different variants of a transmembrane G Protein-Coupled Receptor with a substantially long N-terminus, and we are now at the point where...
28 September 2023 6,229 0 View
Untreated cells are simply cultured cells. Do they indicate cell damage in daily cultured wild-type cells too?
24 September 2023 4,876 1 View
Greetings, I am currently engaged in research involving novel heterostructures composed of various materials. In my investigation, I have observed that the Valence Band Maximum (VBM) and...
21 September 2023 236 2 View