What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Which analysis method in XRD analysis is the most accurate way to measure crystallite size & lattice starin? Scherrer, Williamson-Hall or Rietveld?

Here are three different ways to calculate crystallite size and lattice strain of a sample, based on XRD peaks: "Scherrer" calculation, "Williamson-Hall" or "Rietveld". Each calculation is proper...

04 January 2021 7,836 4 View

Calculate sample size for diagnostic tests?

What sample size formula should I use to compare the three area under the roc curve (AUC) obtained from three diagnostic methods from similar sample? MedCalc software gives us the sample...

20 December 2020 5,635 3 View

How can I produce cell line from human primary fibroblast cells, extracted from synovial Tissue?

I am working on human fibroblast cells from synovial tissue and now they are in 4-5 passages. These cells have been confirmed with specific markers (99% purity). Recently, I have decided to...

17 November 2020 627 3 View

How can I model mechanical properties of a material using Von Misses-Hill model in Abaqus?

Hello everyone; I am trying to simulate a stent which is made of stainless steel in abaqus and in addition to the elastic properties that are defined by Young's modulus and Poisson's ratio I need...

15 September 2020 1,809 4 View

How can I fix this error in HFSS software?

HFSSDesign1 (DrivenModal) [warning] Com Engine non-responsive since 01:04:19, June 08, 2020. Can be due to CPU intensive processing or network problems. If persisting for long, manually...

07 June 2020 2,376 8 View

How can I find about highly cited and hot paper by my affiliation of Nutrition and Food Sciences research center in Scopus site?

How can I find about highly cited and hot paper by my affiliation of Nutrition and Food Sciences research center in Scopus site? I should search and find the number of citation since 3 years ago...

07 January 2020 3,776 10 View

What can people do to reduce personal health risks from air pollution ?

Tehran, the capital city of Iran, suffers from severe air pollution, and it is located near three major fault lines, while being the most populous region of the country. The officials have created...

23 December 2019 7,879 18 View

Is there any affinity between the gold nanoparticles and cellulose acetate filter?

I tried to filter the gold nanoparticle with cellulose acetate, 0.45 um filter. The hydrodynamic size of my nanoparticle are about 100nm (you can see the DLS analysis of them). The gold...

02 October 2019 7,224 7 View

Would you please suggest a suitable and uptodate reference to check the volume of distribution of each drug?

I want to have the volume of distribution of vancomycin but I found variable amounts between 0.5, 0.7, and 0.9 L/Kg in different references. What is the best value in critically ill patients? And...

15 August 2019 5,955 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View