Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

Any recommendations for open-source survey form builder, which has option for semantic differential scale questions apart from Likert scale?

Asking this as Google forms doesn't allow multiple items in a semantic differential format.

04 February 2021 5,869 4 View

Energy minimization of a protein structure with acetylated lysine residues?

I performed in-silico acetylation using PyTM plugin but there are certain clashes that I want to resolve. I couldn't perform energy minimization using gromacs, chimera as they do not have default...

26 September 2020 3,907 6 View

Any ideas why i might be experiencing loss in fiber weight after acetylation?

In recent experiments acetylating cellulose fibers extracted from the leaves of a particular plant, weight loss was experienced. Experiments were repeated several times and reaction mixture was...

18 March 2020 4,599 3 View

Why am I unable to detect Histone PTMs at their correct molecular weight in nuclear extracts via Western Blotting?

I am trying to detect changes in Histone PTMs with or without treatment in nuclear extracts from cell lysates. I use a Hypotonic buffer (20 mM Tris pH 7.4, 10mM NaCl, 1mM MgCl2, 0.5mM EDTA)...

10 March 2020 2,626 6 View

How to perform a MD simulation of a protein with post-translational modifications in Gromacs?

Hi all, I am planing to perform a MD simulation of a protein with two acetylated lysines. Which workflow do you recommend me to follow? Has anyone used Vienna-PTM 2.0? any preferred force...

06 July 2019 8,471 2 View

What are the ways of determine a cognitive changes in muscarinic acetyl choline receptors activation?

Am doing a comparative study of some plant extracts. Pls What are the ways of determine a cognitive changes in muscarinic acetyl choline receptors activation?

05 June 2019 7,098 2 View

Western blot of acetylated EP300/CBP proteins?

Hey all, I am new to WB and I am trying to detect the acetylated form of EP300/CBP but I get extremely faint bands and also almost a smear of unspecific bands. I have tried leaving the Ab...

04 May 2019 7,878 4 View

What could be possible undesired reactions if I start Fmoc removal by PIP/DMF if I had an incomplete washing of the acetylated (AC2O) peptide?

I happened to start Fmoc removal of Peptide using 18,6% Piperidine in DMF before next coupling when the peptide was not well washed after acetylation by acetic anhydride. Could there be any...

02 March 2019 8,038 4 View

Is it possible that acetylation of lignin can result in its solubility in ethanol?

I acetylated lignin and lost all of its precipitate while washing with ethanol

29 January 2019 4,296 3 View

What is the difference between ESI-MS/MS and LC-MS/MS?

I will like to detect and quantify acetylation in Histones and RNA. Which one is preferred?

31 December 2018 8,152 7 View

Could anyone suggest condition for PPD to react with aldehyde, since acetylation of PPD is giving poor results?

Since PPD(p-Phenylenediamine) is a diamine, While doing acetylation of PPD, we are getting multiple byproducts.

31 December 2018 5,869 12 View