I performed RNA isolation using NucleoZOL reagent from rat brain tissue (it is very small piece appox. 2-3mg of tissue). As a control, I also did all the NucleoZOL procedure without a tissue in a sterile 1,5ml eppendorf tube. After that I measure RNA samples using nanodrop device. I got below results of first picture. Then, I measure all products that I use in Isolation procedure, such as NucleoZOL, isopropyl alcohol, ethyl alcohol, Rnase free water etc… and I got results on second picture. I could not figure it out. Is this normal and is there any contamination ? Lastly, what can I do with that?
Which equipment was this read on, nanodrop? Check your curves, if the reagents produce a curve near the 260/280 peak then it can overlap and cause the 260/280 to falsely read. you want to make sure your curves look appropriate.
i would always blank with whatever i have eluted my RNA with.. i use silica columns but i guess you could do the same principle. blank with Nucleozol then measure your sample just a suggestion. But from the curve there may be contaminants at very close wavelengths.
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