I want the procedure for Nitric oxide assay in plant sample.. Please kindly help in this regard at the earliest .. send an email to [email protected].
Hey there Veluchamy Balakrishnan! Nitric oxide assay in plant samples, huh? Well, buckle up, because I got you Veluchamy Balakrishnan covered! First things first, you'll need to gather your gear and prepare for some serious plant chemistry.
1. **Materials:**
- Plant samples (obviously!)
- Griess reagent (sulfanilamide and N-1-naphthylethylenediamine dihydrochloride)
- Phosphate buffer
- Sodium nitrite standard
- Microplate reader
2. **Procedure:**
a. **Sample Preparation:**
- Grind your plant samples into a fine powder.
- Extract the nitric oxide using a suitable buffer.
b. **Reaction Time:**
- Mix the extracted solution with Griess reagent.
- Allow it to react in the dark for about 10-15 minutes.
c. **Measurement:**
- Measure the absorbance at a specific wavelength (around 540 nm) using the microplate reader.
- Compare against a sodium nitrite standard curve to quantify the nitric oxide levels.
3. **Analysis:**
- Calculate nitric oxide concentrations in your samples based on the standard curve.
- Don't forget statistical analysis for that extra scientific flair.
Now, my friend Veluchamy Balakrishnan, you're armed and ready for some serious nitric oxide analysis in your plant samples. Go forth and conquer the world of plant biochemistry! If you Veluchamy Balakrishnan run into any hurdles, you know where to find me for more wisdom.
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